Force Spectroscopy of Hyaluronan by AFM; From H-bonded Networks Towards Single Chain Behavior

M.I. Giannotti; Marguerite Rinaudo; Gyula J. Vancso

doi:10.1021/bm700592j

Force Spectroscopy of Hyaluronan by AFM; From H-bonded Networks Towards Single Chain Behavior

M.I. Giannotti
, Marguerite Rinaudo
, Gyula J. Vancso

Research output: Contribution to journal › Article › Academic › peer-review

59 Citations (Scopus)

16 Downloads (Pure)

Abstract

The conformational behavior of hyaluronan (HA) polysaccharide chains in aqueous NaCl solution was characterized directly at the single-molecule level. This comunication reports on one of the first single-chain atomic force microscopy (AFM) experiments performed at variable temperatures, investigating the influence of the temperature on the stability of the HA single-chain conformation. Through AFM single-molecule force spectroscopy, the temperature destabilization of a local structure was proven. This structure involved a hydrogen-bonded network along the polymeric chain, with hydrogen bonds between the polar groups of HA and possibly water, and a change from a nonrandom coil to a random coil behavior was observed when increasing the temperature from 29 ± 1 to 46 ± 1 °C. As a result of the applied force, this superstructure was found to break progressively at room temperature. The use of a hydrogen-bonding breaker solvent demonstrated the hydrogen-bonded water-bridged nature of the network structure of HA single chains in aqueous NaCl solution.

Original language	Undefined
Pages (from-to)	2648-2652
Number of pages	5
Journal	Biomacromolecules
Volume	8
Issue number	9
DOIs	https://doi.org/10.1021/bm700592j
Publication status	Published - 2007

Keywords

METIS-243274
IR-74702

Access to Document

10.1021/bm700592j

Cite this

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title = "Force Spectroscopy of Hyaluronan by AFM; From H-bonded Networks Towards Single Chain Behavior",

abstract = "The conformational behavior of hyaluronan (HA) polysaccharide chains in aqueous NaCl solution was characterized directly at the single-molecule level. This comunication reports on one of the first single-chain atomic force microscopy (AFM) experiments performed at variable temperatures, investigating the influence of the temperature on the stability of the HA single-chain conformation. Through AFM single-molecule force spectroscopy, the temperature destabilization of a local structure was proven. This structure involved a hydrogen-bonded network along the polymeric chain, with hydrogen bonds between the polar groups of HA and possibly water, and a change from a nonrandom coil to a random coil behavior was observed when increasing the temperature from 29 ± 1 to 46 ± 1 °C. As a result of the applied force, this superstructure was found to break progressively at room temperature. The use of a hydrogen-bonding breaker solvent demonstrated the hydrogen-bonded water-bridged nature of the network structure of HA single chains in aqueous NaCl solution.",

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year = "2007",

doi = "10.1021/bm700592j",

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TY - JOUR

T1 - Force Spectroscopy of Hyaluronan by AFM; From H-bonded Networks Towards Single Chain Behavior

AU - Giannotti, M.I.

AU - Rinaudo, Marguerite

AU - Vancso, Gyula J.

PY - 2007

Y1 - 2007

N2 - The conformational behavior of hyaluronan (HA) polysaccharide chains in aqueous NaCl solution was characterized directly at the single-molecule level. This comunication reports on one of the first single-chain atomic force microscopy (AFM) experiments performed at variable temperatures, investigating the influence of the temperature on the stability of the HA single-chain conformation. Through AFM single-molecule force spectroscopy, the temperature destabilization of a local structure was proven. This structure involved a hydrogen-bonded network along the polymeric chain, with hydrogen bonds between the polar groups of HA and possibly water, and a change from a nonrandom coil to a random coil behavior was observed when increasing the temperature from 29 ± 1 to 46 ± 1 °C. As a result of the applied force, this superstructure was found to break progressively at room temperature. The use of a hydrogen-bonding breaker solvent demonstrated the hydrogen-bonded water-bridged nature of the network structure of HA single chains in aqueous NaCl solution.

AB - The conformational behavior of hyaluronan (HA) polysaccharide chains in aqueous NaCl solution was characterized directly at the single-molecule level. This comunication reports on one of the first single-chain atomic force microscopy (AFM) experiments performed at variable temperatures, investigating the influence of the temperature on the stability of the HA single-chain conformation. Through AFM single-molecule force spectroscopy, the temperature destabilization of a local structure was proven. This structure involved a hydrogen-bonded network along the polymeric chain, with hydrogen bonds between the polar groups of HA and possibly water, and a change from a nonrandom coil to a random coil behavior was observed when increasing the temperature from 29 ± 1 to 46 ± 1 °C. As a result of the applied force, this superstructure was found to break progressively at room temperature. The use of a hydrogen-bonding breaker solvent demonstrated the hydrogen-bonded water-bridged nature of the network structure of HA single chains in aqueous NaCl solution.

KW - METIS-243274

KW - IR-74702

U2 - 10.1021/bm700592j

DO - 10.1021/bm700592j

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