Free Flow Ion Concentration Polarization Focusing (FF-ICPF)

Vasileios A. Papadimitriou; Loes I. Segerink; Jan C.T. Eijkel

doi:10.1021/acs.analchem.9b04526

Free Flow Ion Concentration Polarization Focusing (FF-ICPF)

Vasileios A. Papadimitriou, Loes I. Segerink, Jan C.T. Eijkel

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Electrokinetic separation techniques in microfluidics are a powerful analytical chemistry tool, although an inherent limitation of microfluidics is their low sample throughput. In this article we report a free-flow variant of an electrokinetic focusing method, namely ion concentration polarization focusing (ICPF). The analytes flow continuously through the system via pressure driven flow while they separate and concentrate perpendicularly to the flow by ICPF. We demonstrate free flow ion concentration polarization focusing (FF-ICPF) in two operating modes, namely peak and plateau modes. Additionally, we showed the separation resolution could be improved by the use of an electrophoretic spacer. We report a concentration factor of 10 in human blood plasma in continuous flow at a flow rate of 15 μL min–1.

Original language	English
Pages (from-to)	4866–4874
Number of pages	9
Journal	Analytical chemistry
Volume	92
Issue number	7
DOIs	https://doi.org/10.1021/acs.analchem.9b04526
Publication status	Published - 20 Mar 2020

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UT-Hybrid-D

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abstract = "Electrokinetic separation techniques in microfluidics are a powerful analytical chemistry tool, although an inherent limitation of microfluidics is their low sample throughput. In this article we report a free-flow variant of an electrokinetic focusing method, namely ion concentration polarization focusing (ICPF). The analytes flow continuously through the system via pressure driven flow while they separate and concentrate perpendicularly to the flow by ICPF. We demonstrate free flow ion concentration polarization focusing (FF-ICPF) in two operating modes, namely peak and plateau modes. Additionally, we showed the separation resolution could be improved by the use of an electrophoretic spacer. We report a concentration factor of 10 in human blood plasma in continuous flow at a flow rate of 15 μL min–1.",

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T1 - Free Flow Ion Concentration Polarization Focusing (FF-ICPF)

AU - Papadimitriou, Vasileios A.

AU - Segerink, Loes I.

AU - Eijkel, Jan C.T.

N1 - ACS deal

PY - 2020/3/20

Y1 - 2020/3/20

N2 - Electrokinetic separation techniques in microfluidics are a powerful analytical chemistry tool, although an inherent limitation of microfluidics is their low sample throughput. In this article we report a free-flow variant of an electrokinetic focusing method, namely ion concentration polarization focusing (ICPF). The analytes flow continuously through the system via pressure driven flow while they separate and concentrate perpendicularly to the flow by ICPF. We demonstrate free flow ion concentration polarization focusing (FF-ICPF) in two operating modes, namely peak and plateau modes. Additionally, we showed the separation resolution could be improved by the use of an electrophoretic spacer. We report a concentration factor of 10 in human blood plasma in continuous flow at a flow rate of 15 μL min–1.

AB - Electrokinetic separation techniques in microfluidics are a powerful analytical chemistry tool, although an inherent limitation of microfluidics is their low sample throughput. In this article we report a free-flow variant of an electrokinetic focusing method, namely ion concentration polarization focusing (ICPF). The analytes flow continuously through the system via pressure driven flow while they separate and concentrate perpendicularly to the flow by ICPF. We demonstrate free flow ion concentration polarization focusing (FF-ICPF) in two operating modes, namely peak and plateau modes. Additionally, we showed the separation resolution could be improved by the use of an electrophoretic spacer. We report a concentration factor of 10 in human blood plasma in continuous flow at a flow rate of 15 μL min–1.

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JO - Analytical chemistry

JF - Analytical chemistry

IS - 7

ER -

Free Flow Ion Concentration Polarization Focusing (FF-ICPF)

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