This thesis presents a body of interdisciplinary research aimed at visualizing bone tissue engineering using microbioreactors and non-invasive and label-free vibrational Raman microspectroscopy. Confocal Raman microspectroscopy is an optical microspectroscopic technique enabling spatially resolved chemical imaging and analysis. In this thesis this approach was used to monitor the temporal development of in vitro bone formation, that is, from proliferation of human bone marrow derived stromal cells, their differentiation towards osteogenic precursors, till early and late stages of mineralized tissue formation. The results yield deeper insights into, and understanding of, in vitro formation of mineralized tissue from stromal cells derived from human bone marrow that are consistent with the characteristics of in vivo bone formation.
|Award date||19 Mar 2010|
|Place of Publication||Enschede|
|Publication status||Published - 19 Mar 2010|