Abstract
This thesis presents a body of interdisciplinary research aimed at visualizing bone
tissue engineering using microbioreactors and non-invasive and label-free vibrational Raman
microspectroscopy. Confocal Raman microspectroscopy is an optical microspectroscopic
technique enabling spatially resolved chemical imaging and analysis. In this thesis this
approach was used to monitor the temporal development of in vitro bone formation, that is,
from proliferation of human bone marrow derived stromal cells, their differentiation towards
osteogenic precursors, till early and late stages of mineralized tissue formation. The results
yield deeper insights into, and understanding of, in vitro formation of mineralized tissue from
stromal cells derived from human bone marrow that are consistent with the characteristics of
in vivo bone formation.
Original language | English |
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Awarding Institution |
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Supervisors/Advisors |
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Award date | 19 Mar 2010 |
Place of Publication | Enschede |
Publisher | |
Print ISBNs | 978-90-365-2993-8 |
DOIs | |
Publication status | Published - 19 Mar 2010 |
Keywords
- IR-70336