From cells to bone: raman microspectroscopy of the mineralization of stromal cells

Vishnu Vardhan Pully

doi:10.3990/1.9789036529938

From cells to bone: raman microspectroscopy of the mineralization of stromal cells

Vishnu Vardhan Pully

Nanobiophysics

Research output: Thesis › PhD Thesis - Research UT, graduation UT

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Abstract

This thesis presents a body of interdisciplinary research aimed at visualizing bone tissue engineering using microbioreactors and non-invasive and label-free vibrational Raman microspectroscopy. Confocal Raman microspectroscopy is an optical microspectroscopic technique enabling spatially resolved chemical imaging and analysis. In this thesis this approach was used to monitor the temporal development of in vitro bone formation, that is, from proliferation of human bone marrow derived stromal cells, their differentiation towards osteogenic precursors, till early and late stages of mineralized tissue formation. The results yield deeper insights into, and understanding of, in vitro formation of mineralized tissue from stromal cells derived from human bone marrow that are consistent with the characteristics of in vivo bone formation.

Original language	English
Awarding Institution	University of Twente
Supervisors/Advisors	Subramaniam, V., Supervisor Otto, Cees, Supervisor
Award date	19 Mar 2010
Place of Publication	Enschede
Publisher	University of Twente
Print ISBNs	978-90-365-2993-8
DOIs	https://doi.org/10.3990/1.9789036529938
Publication status	Published - 19 Mar 2010

Keywords

IR-70336

Access to Document

10.3990/1.9789036529938

thesis_V_PullyFinal published version, 14.9 MB

Cite this

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title = "From cells to bone: raman microspectroscopy of the mineralization of stromal cells",

abstract = "This thesis presents a body of interdisciplinary research aimed at visualizing bone tissue engineering using microbioreactors and non-invasive and label-free vibrational Raman microspectroscopy. Confocal Raman microspectroscopy is an optical microspectroscopic technique enabling spatially resolved chemical imaging and analysis. In this thesis this approach was used to monitor the temporal development of in vitro bone formation, that is, from proliferation of human bone marrow derived stromal cells, their differentiation towards osteogenic precursors, till early and late stages of mineralized tissue formation. The results yield deeper insights into, and understanding of, in vitro formation of mineralized tissue from stromal cells derived from human bone marrow that are consistent with the characteristics of in vivo bone formation.",

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N2 - This thesis presents a body of interdisciplinary research aimed at visualizing bone tissue engineering using microbioreactors and non-invasive and label-free vibrational Raman microspectroscopy. Confocal Raman microspectroscopy is an optical microspectroscopic technique enabling spatially resolved chemical imaging and analysis. In this thesis this approach was used to monitor the temporal development of in vitro bone formation, that is, from proliferation of human bone marrow derived stromal cells, their differentiation towards osteogenic precursors, till early and late stages of mineralized tissue formation. The results yield deeper insights into, and understanding of, in vitro formation of mineralized tissue from stromal cells derived from human bone marrow that are consistent with the characteristics of in vivo bone formation.

AB - This thesis presents a body of interdisciplinary research aimed at visualizing bone tissue engineering using microbioreactors and non-invasive and label-free vibrational Raman microspectroscopy. Confocal Raman microspectroscopy is an optical microspectroscopic technique enabling spatially resolved chemical imaging and analysis. In this thesis this approach was used to monitor the temporal development of in vitro bone formation, that is, from proliferation of human bone marrow derived stromal cells, their differentiation towards osteogenic precursors, till early and late stages of mineralized tissue formation. The results yield deeper insights into, and understanding of, in vitro formation of mineralized tissue from stromal cells derived from human bone marrow that are consistent with the characteristics of in vivo bone formation.

KW - IR-70336

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M3 - PhD Thesis - Research UT, graduation UT

SN - 978-90-365-2993-8

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