From cells to bone: raman microspectroscopy of the mineralization of stromal cells

Vishnu Vardhan Pully

Research output: ThesisPhD Thesis - Research UT, graduation UT

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This thesis presents a body of interdisciplinary research aimed at visualizing bone tissue engineering using microbioreactors and non-invasive and label-free vibrational Raman microspectroscopy. Confocal Raman microspectroscopy is an optical microspectroscopic technique enabling spatially resolved chemical imaging and analysis. In this thesis this approach was used to monitor the temporal development of in vitro bone formation, that is, from proliferation of human bone marrow derived stromal cells, their differentiation towards osteogenic precursors, till early and late stages of mineralized tissue formation. The results yield deeper insights into, and understanding of, in vitro formation of mineralized tissue from stromal cells derived from human bone marrow that are consistent with the characteristics of in vivo bone formation.
Original languageEnglish
Awarding Institution
  • University of Twente
  • Subramaniam, V., Supervisor
  • Otto, Cees, Supervisor
Award date19 Mar 2010
Place of PublicationEnschede
Print ISBNs978-90-365-2993-8
Publication statusPublished - 19 Mar 2010


  • IR-70336

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