TY - JOUR
T1 - Functional expression of ZNF467 and PCBP2 supports adipogenic lineage commitment in adipose-derived mesenchymal stem cells
AU - Gluscevic, Martina
AU - Paradise, Christopher R.
AU - Dudakovic, Amel
AU - Karperien, Marcel
AU - Dietz, Allan B.
AU - van Wijnen, Andre J.
AU - Deyle, David R.
PY - 2020/5/5
Y1 - 2020/5/5
N2 - Bone marrow-derived mesenchymal stromal/stem cells (BMSCs) have the potential to be employed in many different skeletal therapies. A major limitation to utilizing BMSCs as a therapeutic strategy in human disease and tissue regeneration is the low cell numbers obtained from initial isolation necessitating multiple cell passages that can lead to decreased cell quality. Adipose-derived mesenchymal stromal/stem cells (AMSCs) have been proposed as an alternative cell source for regenerative therapies; however the differentiation capacity of these cells differs from BMSCs. To understand the differences between BMSCs and AMSCs, we compared the global gene expression profiles of BMSCs and AMSCs and identified two genes, PCBP2 and ZNF467 that were differentially expressed between AMSCs and BMSCs. We demonstrate that PCBP2 and ZNF467 impact adipogenic but not osteogenic differentiation, further supporting evidence that AMSCs and BMSCs appear to be adapted to their microenvironment.
AB - Bone marrow-derived mesenchymal stromal/stem cells (BMSCs) have the potential to be employed in many different skeletal therapies. A major limitation to utilizing BMSCs as a therapeutic strategy in human disease and tissue regeneration is the low cell numbers obtained from initial isolation necessitating multiple cell passages that can lead to decreased cell quality. Adipose-derived mesenchymal stromal/stem cells (AMSCs) have been proposed as an alternative cell source for regenerative therapies; however the differentiation capacity of these cells differs from BMSCs. To understand the differences between BMSCs and AMSCs, we compared the global gene expression profiles of BMSCs and AMSCs and identified two genes, PCBP2 and ZNF467 that were differentially expressed between AMSCs and BMSCs. We demonstrate that PCBP2 and ZNF467 impact adipogenic but not osteogenic differentiation, further supporting evidence that AMSCs and BMSCs appear to be adapted to their microenvironment.
KW - Adipogenic differentiation
KW - Adipose-derived mesenchymal stromal/stem cells
KW - 22/2 OA procedure
UR - http://www.scopus.com/inward/record.url?scp=85079387674&partnerID=8YFLogxK
U2 - 10.1016/j.gene.2020.144437
DO - 10.1016/j.gene.2020.144437
M3 - Article
C2 - 32032745
AN - SCOPUS:85079387674
SN - 0378-1119
VL - 737
JO - Gene
JF - Gene
M1 - 144437
ER -
