Functionalization and bioimmobilization of silicon surfaces with Si-N bonded monolayer

M.N. Masood, Edwin Carlen, Albert van den Berg

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

A new method for selective surface functionalization of silicon with a silicon-nitrogen bonded (Si-N) monolayer and subsequent bioimmobilization is presented. The Si-N monolayer was studied using Xray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), fluorescence spectroscopy, and capacitance-voltage (C-V) measurements using an electrolyte insulator semiconductor (EIS) sensor. A symmetric ethylene diamine (EDA, C2H4 (NH2)(2)) molecule was used as a precursor compound for monolayer formation in an inert environment in the liquid phase. XPS results show the attachment of EDA molecules proceeds via dissociative and dative bond formation whereas free amine groups on the surface were used for further immobilization of biomolecules. SEM shows selective functionalization and bioimmobilization on a patterned surface such as a silicon nanowire on silicon oxide substrate. Biosensing experiments with bioimmobilized surfaces were carried out in aqueous buffer and show high sensitivity for biosensing. (C) 2015 Elsevier B.V. All rights reserved.
Original languageUndefined
Pages (from-to)105-110
Number of pages6
JournalApplied surface science
Volume337
DOIs
Publication statusPublished - 15 May 2015

Keywords

  • EWI-26010
  • METIS-312602
  • IR-95881

Cite this

@article{fd6e54f47b3c47348609ae0a2312f17a,
title = "Functionalization and bioimmobilization of silicon surfaces with Si-N bonded monolayer",
abstract = "A new method for selective surface functionalization of silicon with a silicon-nitrogen bonded (Si-N) monolayer and subsequent bioimmobilization is presented. The Si-N monolayer was studied using Xray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), fluorescence spectroscopy, and capacitance-voltage (C-V) measurements using an electrolyte insulator semiconductor (EIS) sensor. A symmetric ethylene diamine (EDA, C2H4 (NH2)(2)) molecule was used as a precursor compound for monolayer formation in an inert environment in the liquid phase. XPS results show the attachment of EDA molecules proceeds via dissociative and dative bond formation whereas free amine groups on the surface were used for further immobilization of biomolecules. SEM shows selective functionalization and bioimmobilization on a patterned surface such as a silicon nanowire on silicon oxide substrate. Biosensing experiments with bioimmobilized surfaces were carried out in aqueous buffer and show high sensitivity for biosensing. (C) 2015 Elsevier B.V. All rights reserved.",
keywords = "EWI-26010, METIS-312602, IR-95881",
author = "M.N. Masood and Edwin Carlen and {van den Berg}, Albert",
note = "eemcs-eprint-26010",
year = "2015",
month = "5",
day = "15",
doi = "10.1016/j.apsusc.2015.02.069",
language = "Undefined",
volume = "337",
pages = "105--110",
journal = "Applied surface science",
issn = "0169-4332",
publisher = "Elsevier",

}

Functionalization and bioimmobilization of silicon surfaces with Si-N bonded monolayer. / Masood, M.N.; Carlen, Edwin; van den Berg, Albert.

In: Applied surface science, Vol. 337, 15.05.2015, p. 105-110.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Functionalization and bioimmobilization of silicon surfaces with Si-N bonded monolayer

AU - Masood, M.N.

AU - Carlen, Edwin

AU - van den Berg, Albert

N1 - eemcs-eprint-26010

PY - 2015/5/15

Y1 - 2015/5/15

N2 - A new method for selective surface functionalization of silicon with a silicon-nitrogen bonded (Si-N) monolayer and subsequent bioimmobilization is presented. The Si-N monolayer was studied using Xray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), fluorescence spectroscopy, and capacitance-voltage (C-V) measurements using an electrolyte insulator semiconductor (EIS) sensor. A symmetric ethylene diamine (EDA, C2H4 (NH2)(2)) molecule was used as a precursor compound for monolayer formation in an inert environment in the liquid phase. XPS results show the attachment of EDA molecules proceeds via dissociative and dative bond formation whereas free amine groups on the surface were used for further immobilization of biomolecules. SEM shows selective functionalization and bioimmobilization on a patterned surface such as a silicon nanowire on silicon oxide substrate. Biosensing experiments with bioimmobilized surfaces were carried out in aqueous buffer and show high sensitivity for biosensing. (C) 2015 Elsevier B.V. All rights reserved.

AB - A new method for selective surface functionalization of silicon with a silicon-nitrogen bonded (Si-N) monolayer and subsequent bioimmobilization is presented. The Si-N monolayer was studied using Xray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), fluorescence spectroscopy, and capacitance-voltage (C-V) measurements using an electrolyte insulator semiconductor (EIS) sensor. A symmetric ethylene diamine (EDA, C2H4 (NH2)(2)) molecule was used as a precursor compound for monolayer formation in an inert environment in the liquid phase. XPS results show the attachment of EDA molecules proceeds via dissociative and dative bond formation whereas free amine groups on the surface were used for further immobilization of biomolecules. SEM shows selective functionalization and bioimmobilization on a patterned surface such as a silicon nanowire on silicon oxide substrate. Biosensing experiments with bioimmobilized surfaces were carried out in aqueous buffer and show high sensitivity for biosensing. (C) 2015 Elsevier B.V. All rights reserved.

KW - EWI-26010

KW - METIS-312602

KW - IR-95881

U2 - 10.1016/j.apsusc.2015.02.069

DO - 10.1016/j.apsusc.2015.02.069

M3 - Article

VL - 337

SP - 105

EP - 110

JO - Applied surface science

JF - Applied surface science

SN - 0169-4332

ER -