Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads

Albert Blanch-Asensio; Babet van der Vaart; Mariana Vinagre; Eline Groen; Christiaan Arendzen; Christian Freund; Niels Geijsen; Christine L. Mummery; Richard P. Davis

doi:10.1016/j.scr.2022.102991

Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads

Albert Blanch-Asensio
, Babet van der Vaart
, Mariana Vinagre
, Eline Groen
, Christiaan Arendzen
, Christian Freund
, Niels Geijsen
, Christine L. Mummery
, Richard P. Davis^*

^*Corresponding author for this work

Applied Stem Cell Technologies

Research output: Contribution to journal › Article › Academic › peer-review

12 Citations (Scopus)

135 Downloads (Pure)

Abstract

STRAIGHT-IN is a platform to precisely integrate DNA payloads into the genome of cells, including hiPSCs. Here, we generated two hiPSC acceptor lines each with one copy of an upgraded landing pad (LP). This improved design allows more efficient (∼100 %) and rapid (∼2–3 weeks) generation of genetically modified hiPSC lines containing the desired payloads. This new LP version was inserted into either the AAVS1 (LUMCi004-A-1) or CLYBL (LUMCi004-A-2) safe harbour loci in the hiPSC line, LUMC0099iCTRL04. The resulting lines can be used for the targeted integration of a wide range of transgenes, thereby making them suitable for numerous research applications.

Original language	English
Article number	102991
Journal	Stem cell research
Volume	66
DOIs	https://doi.org/10.1016/j.scr.2022.102991
Publication status	Published - Feb 2023

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10.1016/j.scr.2022.102991Licence: CC BY

1-s2.0-S1873506122003403-mainFinal published version, 4.31 MBLicence: CC BY

Cite this

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title = "Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads",

abstract = "STRAIGHT-IN is a platform to precisely integrate DNA payloads into the genome of cells, including hiPSCs. Here, we generated two hiPSC acceptor lines each with one copy of an upgraded landing pad (LP). This improved design allows more efficient (∼100 \%) and rapid (∼2–3 weeks) generation of genetically modified hiPSC lines containing the desired payloads. This new LP version was inserted into either the AAVS1 (LUMCi004-A-1) or CLYBL (LUMCi004-A-2) safe harbour loci in the hiPSC line, LUMC0099iCTRL04. The resulting lines can be used for the targeted integration of a wide range of transgenes, thereby making them suitable for numerous research applications.",

author = "Albert Blanch-Asensio and \{van der Vaart\}, Babet and Mariana Vinagre and Eline Groen and Christiaan Arendzen and Christian Freund and Niels Geijsen and Mummery, \{Christine L.\} and Davis, \{Richard P.\}",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2023",

month = feb,

doi = "10.1016/j.scr.2022.102991",

language = "English",

volume = "66",

journal = "Stem cell research",

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TY - JOUR

T1 - Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads

AU - Blanch-Asensio, Albert

AU - van der Vaart, Babet

AU - Vinagre, Mariana

AU - Groen, Eline

AU - Arendzen, Christiaan

AU - Freund, Christian

AU - Geijsen, Niels

AU - Mummery, Christine L.

AU - Davis, Richard P.

PY - 2023/2

Y1 - 2023/2

N2 - STRAIGHT-IN is a platform to precisely integrate DNA payloads into the genome of cells, including hiPSCs. Here, we generated two hiPSC acceptor lines each with one copy of an upgraded landing pad (LP). This improved design allows more efficient (∼100 %) and rapid (∼2–3 weeks) generation of genetically modified hiPSC lines containing the desired payloads. This new LP version was inserted into either the AAVS1 (LUMCi004-A-1) or CLYBL (LUMCi004-A-2) safe harbour loci in the hiPSC line, LUMC0099iCTRL04. The resulting lines can be used for the targeted integration of a wide range of transgenes, thereby making them suitable for numerous research applications.

AB - STRAIGHT-IN is a platform to precisely integrate DNA payloads into the genome of cells, including hiPSCs. Here, we generated two hiPSC acceptor lines each with one copy of an upgraded landing pad (LP). This improved design allows more efficient (∼100 %) and rapid (∼2–3 weeks) generation of genetically modified hiPSC lines containing the desired payloads. This new LP version was inserted into either the AAVS1 (LUMCi004-A-1) or CLYBL (LUMCi004-A-2) safe harbour loci in the hiPSC line, LUMC0099iCTRL04. The resulting lines can be used for the targeted integration of a wide range of transgenes, thereby making them suitable for numerous research applications.

UR - https://www.scopus.com/pages/publications/85143983866

U2 - 10.1016/j.scr.2022.102991

DO - 10.1016/j.scr.2022.102991

M3 - Article

C2 - 36495703

AN - SCOPUS:85143983866

SN - 1873-5061

VL - 66

JO - Stem cell research

JF - Stem cell research

M1 - 102991

ER -

Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads

Abstract

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