Ghrelin expression in dissociated cultures, of the rat neocortex

Irina Stoyanova; Remy Wiertz; Wim Rutten

doi:10.1109/NER.2009.5109259

Ghrelin expression in dissociated cultures, of the rat neocortex

Irina Stoyanova, Remy Wiertz, Wim Rutten

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

3 Citations (Scopus)

20 Downloads (Pure)

Abstract

Ghrelin is a hormone, initially described as a gastric peptide stimulating appetite and growth hormone secretrion, which also has an important role in the regulation of many other processes including higher brain functions. Ghrelin has been described in situ in different part of the brain, but so far there are no data about it in cell cultures. Therefore, we aimed this study in order to investigate the developmental pattern of ghrelin in dissociated cortical neurons. We applied the ABC immunocytochemical mehtod for detection of ghrelin in dissociated cortical neurons from newborn rats, incubated for one day, one week, or two weeks. Our results clearly show that at the early stages of development of the network all the neurons are ghrelin-positive and their number decreases along the incubation period. As ghrelin is so omnipresent in the early stages, it may also influence the early development of synaptic formation and cell-to-cell interactions, both very important for the network functions like learning and memory.

Original language	Undefined
Title of host publication	Proceeding of the 4th International IEEE EMBS Conference on Neural Engineering
Place of Publication	Piscataway
Publisher	IEEE Engineering in Medicine and Biology Society
Pages	159-162
Number of pages	4
ISBN (Print)	978-1-4244-2073-5
DOIs	https://doi.org/10.1109/NER.2009.5109259
Publication status	Published - 29 Apr 2009
Event	4th International IEEE/EMBS Conference on Neural Engineering, NER 2009 - Antalya, Turkey Duration: 29 Apr 2009 → 2 May 2009 Conference number: 4

Publication series

Name
Publisher	IEEE-EMBS

Conference

Conference	4th International IEEE/EMBS Conference on Neural Engineering, NER 2009
Abbreviated title	NER
Country	Turkey
City	Antalya
Period	29/04/09 → 2/05/09

Keywords

EWI-15365
ghrelin expression dissociated cultures rat neocortex
dissociated cultures
ghrelin development
rat neocortex
METIS-265208
Dissociated cortical neurons
Ghrelin expression
Learning
Memory
IR-62811

Access to Document

10.1109/NER.2009.5109259

040_2708stoyanova
open

Cite this

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title = "Ghrelin expression in dissociated cultures, of the rat neocortex",

abstract = "Ghrelin is a hormone, initially described as a gastric peptide stimulating appetite and growth hormone secretrion, which also has an important role in the regulation of many other processes including higher brain functions. Ghrelin has been described in situ in different part of the brain, but so far there are no data about it in cell cultures. Therefore, we aimed this study in order to investigate the developmental pattern of ghrelin in dissociated cortical neurons. We applied the ABC immunocytochemical mehtod for detection of ghrelin in dissociated cortical neurons from newborn rats, incubated for one day, one week, or two weeks. Our results clearly show that at the early stages of development of the network all the neurons are ghrelin-positive and their number decreases along the incubation period. As ghrelin is so omnipresent in the early stages, it may also influence the early development of synaptic formation and cell-to-cell interactions, both very important for the network functions like learning and memory.",

keywords = "EWI-15365, ghrelin expression dissociated cultures rat neocortex, dissociated cultures, ghrelin development, rat neocortex, METIS-265208, Dissociated cortical neurons, Ghrelin expression, Learning, Memory, IR-62811",

author = "Irina Stoyanova and Remy Wiertz and Wim Rutten",

year = "2009",

month = apr,

day = "29",

doi = "10.1109/NER.2009.5109259",

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publisher = "IEEE Engineering in Medicine and Biology Society",

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booktitle = "Proceeding of the 4th International IEEE EMBS Conference on Neural Engineering",

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Stoyanova, I, Wiertz, R & Rutten, W 2009, Ghrelin expression in dissociated cultures, of the rat neocortex. in Proceeding of the 4th International IEEE EMBS Conference on Neural Engineering. IEEE Engineering in Medicine and Biology Society, Piscataway, pp. 159-162, 4th International IEEE/EMBS Conference on Neural Engineering, NER 2009, Antalya, Turkey, 29/04/09. https://doi.org/10.1109/NER.2009.5109259

Ghrelin expression in dissociated cultures, of the rat neocortex. / Stoyanova, Irina; Wiertz, Remy; Rutten, Wim.

Proceeding of the 4th International IEEE EMBS Conference on Neural Engineering. Piscataway : IEEE Engineering in Medicine and Biology Society, 2009. p. 159-162.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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AU - Wiertz, Remy

AU - Rutten, Wim

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Y1 - 2009/4/29

N2 - Ghrelin is a hormone, initially described as a gastric peptide stimulating appetite and growth hormone secretrion, which also has an important role in the regulation of many other processes including higher brain functions. Ghrelin has been described in situ in different part of the brain, but so far there are no data about it in cell cultures. Therefore, we aimed this study in order to investigate the developmental pattern of ghrelin in dissociated cortical neurons. We applied the ABC immunocytochemical mehtod for detection of ghrelin in dissociated cortical neurons from newborn rats, incubated for one day, one week, or two weeks. Our results clearly show that at the early stages of development of the network all the neurons are ghrelin-positive and their number decreases along the incubation period. As ghrelin is so omnipresent in the early stages, it may also influence the early development of synaptic formation and cell-to-cell interactions, both very important for the network functions like learning and memory.

AB - Ghrelin is a hormone, initially described as a gastric peptide stimulating appetite and growth hormone secretrion, which also has an important role in the regulation of many other processes including higher brain functions. Ghrelin has been described in situ in different part of the brain, but so far there are no data about it in cell cultures. Therefore, we aimed this study in order to investigate the developmental pattern of ghrelin in dissociated cortical neurons. We applied the ABC immunocytochemical mehtod for detection of ghrelin in dissociated cortical neurons from newborn rats, incubated for one day, one week, or two weeks. Our results clearly show that at the early stages of development of the network all the neurons are ghrelin-positive and their number decreases along the incubation period. As ghrelin is so omnipresent in the early stages, it may also influence the early development of synaptic formation and cell-to-cell interactions, both very important for the network functions like learning and memory.

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