Glucose level determination with a multi-enzymatic cascade reaction in a functionalized glass chip

Francesca Costantini; Roald M. Tiggelaar; Simona Sennato; Francesco Mura; Stefan Schlautmann; Frederico Bordi; Johannes G.E. Gardeniers; Cesare Manetti

doi:10.1039/c3an00806a

Glucose level determination with a multi-enzymatic cascade reaction in a functionalized glass chip

Francesca Costantini, Roald M. Tiggelaar, Simona Sennato, Francesco Mura, Stefan Schlautmann, Frederico Bordi, Johannes G.E. Gardeniers, Cesare Manetti^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

In this work we show the functionalization of the interior of microfluidic glass chips with poly(2-hydroxyethyl methacrylate) polymer brushes as anchors for co-immobilization of the enzymes glucose-oxidase and horseradish peroxidase. The formation of the brush layer and subsequent immobilization of these enzymes have been characterized on flat surfaces by atomic force microscopy and Fourier transform infrared spectroscopy, and studied inside glass chips by field emission scanning microscopy. Enzyme-functionalized glass chips have been applied for performing a multi-enzymatic cascade reaction for the fast (20 s) determination of glucose in human blood samples and the result is in excellent agreement with values obtained from the conventional hospital laboratory. The limit of detection of this bi-enzymatic method is 60 μM. With the advantages of high selectivity and reproducibility, this functionalization method can be used for improving the efficiency of glucose sensors.

Original language	English
Pages (from-to)	5019-5024
Number of pages	6
Journal	Analyst
Volume	138
Issue number	17
DOIs	https://doi.org/10.1039/c3an00806a
Publication status	Published - 2013

Keywords

2024 OA procedure

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10.1039/c3an00806a

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title = "Glucose level determination with a multi-enzymatic cascade reaction in a functionalized glass chip",

abstract = "In this work we show the functionalization of the interior of microfluidic glass chips with poly(2-hydroxyethyl methacrylate) polymer brushes as anchors for co-immobilization of the enzymes glucose-oxidase and horseradish peroxidase. The formation of the brush layer and subsequent immobilization of these enzymes have been characterized on flat surfaces by atomic force microscopy and Fourier transform infrared spectroscopy, and studied inside glass chips by field emission scanning microscopy. Enzyme-functionalized glass chips have been applied for performing a multi-enzymatic cascade reaction for the fast (20 s) determination of glucose in human blood samples and the result is in excellent agreement with values obtained from the conventional hospital laboratory. The limit of detection of this bi-enzymatic method is 60 μM. With the advantages of high selectivity and reproducibility, this functionalization method can be used for improving the efficiency of glucose sensors.",

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year = "2013",

doi = "10.1039/c3an00806a",

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T1 - Glucose level determination with a multi-enzymatic cascade reaction in a functionalized glass chip

AU - Costantini, Francesca

AU - Tiggelaar, Roald M.

AU - Sennato, Simona

AU - Mura, Francesco

AU - Schlautmann, Stefan

AU - Bordi, Frederico

AU - Gardeniers, Johannes G.E.

AU - Manetti, Cesare

PY - 2013

Y1 - 2013

N2 - In this work we show the functionalization of the interior of microfluidic glass chips with poly(2-hydroxyethyl methacrylate) polymer brushes as anchors for co-immobilization of the enzymes glucose-oxidase and horseradish peroxidase. The formation of the brush layer and subsequent immobilization of these enzymes have been characterized on flat surfaces by atomic force microscopy and Fourier transform infrared spectroscopy, and studied inside glass chips by field emission scanning microscopy. Enzyme-functionalized glass chips have been applied for performing a multi-enzymatic cascade reaction for the fast (20 s) determination of glucose in human blood samples and the result is in excellent agreement with values obtained from the conventional hospital laboratory. The limit of detection of this bi-enzymatic method is 60 μM. With the advantages of high selectivity and reproducibility, this functionalization method can be used for improving the efficiency of glucose sensors.

AB - In this work we show the functionalization of the interior of microfluidic glass chips with poly(2-hydroxyethyl methacrylate) polymer brushes as anchors for co-immobilization of the enzymes glucose-oxidase and horseradish peroxidase. The formation of the brush layer and subsequent immobilization of these enzymes have been characterized on flat surfaces by atomic force microscopy and Fourier transform infrared spectroscopy, and studied inside glass chips by field emission scanning microscopy. Enzyme-functionalized glass chips have been applied for performing a multi-enzymatic cascade reaction for the fast (20 s) determination of glucose in human blood samples and the result is in excellent agreement with values obtained from the conventional hospital laboratory. The limit of detection of this bi-enzymatic method is 60 μM. With the advantages of high selectivity and reproducibility, this functionalization method can be used for improving the efficiency of glucose sensors.

KW - 2024 OA procedure

U2 - 10.1039/c3an00806a

DO - 10.1039/c3an00806a

M3 - Article

SN - 0003-2654

VL - 138

SP - 5019

EP - 5024

JO - Analyst

JF - Analyst

IS - 17

ER -

Glucose level determination with a multi-enzymatic cascade reaction in a functionalized glass chip

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