Glucose level determination with a multi-enzymatic cascade reaction in a functionalized glass chip

F. Costantini, Roald M. Tiggelaar, S. Sennato, F. Mura, Stefan Schlautmann, F. Bordi, Johannes G.E. Gardeniers, C. Manetti

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)

Abstract

In this work we show the functionalization of the interior of microfluidic glass chips with poly(2-hydroxyethyl methacrylate) polymer brushes as anchors for co-immobilization of the enzymes glucose-oxidase and horseradish peroxidase. The formation of the brush layer and subsequent immobilization of these enzymes have been characterized on flat surfaces by atomic force microscopy and Fourier transform infrared spectroscopy, and studied inside glass chips by field emission scanning microscopy. Enzyme-functionalized glass chips have been applied for performing a multi-enzymatic cascade reaction for the fast (20 s) determination of glucose in human blood samples and the result is in excellent agreement with values obtained from the conventional hospital laboratory. The limit of detection of this bi-enzymatic method is 60 μM. With the advantages of high selectivity and reproducibility, this functionalization method can be used for improving the efficiency of glucose sensors.
Original languageEnglish
Pages (from-to)5019-5024
Number of pages6
JournalAnalyst
Volume138
Issue number17
DOIs
Publication statusPublished - 2013

Keywords

  • METIS-296998
  • IR-86903

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    Costantini, F., Tiggelaar, R. M., Sennato, S., Mura, F., Schlautmann, S., Bordi, F., ... Manetti, C. (2013). Glucose level determination with a multi-enzymatic cascade reaction in a functionalized glass chip. Analyst, 138(17), 5019-5024. https://doi.org/10.1039/c3an00806a