Glucose Single-Chain Polymer Nanoparticles for Cellular Targeting

A. Pia P. Kröger, Muhabbat I. Komil, Naomi M. Hamelmann, Alberto Juan, Martina H. Stenzel, Jos M.J. Paulusse (Corresponding Author)

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4 Citations (Scopus)
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Abstract

Naturally occurring glycoconjugates possess carbohydrate moieties that fulfill essential roles in many biological functions. Through conjugation of carbohydrates to therapeutics or imaging agents, naturally occurring glycoconjugates are mimicked and efficient targeting or increased cellular uptake of glycoconjugated macromolecules is achieved. In this work, linear and cyclic glucose moieties were functionalized with methacrylates via enzymatic synthesis and used as building blocks for intramolecular cross-linked single-chain glycopolymer nanoparticles (glyco-SCNPs). A set of water-soluble sub-10 nm-sized glyco-SCNPs was prepared by thiol-Michael addition cross-linking in water. Bioactivity of various glucose-conjugated glycopolymers and glyco-SCNPs was evaluated in binding studies with the glucose-specific lectin Concanavalin A and by comparing their cellular uptake efficiency in HeLa cells. Cytotoxicity studies did not reveal discernible cytotoxic effects, making these SCNPs promising candidates for ligand-based targeted imaging and drug delivery.

Original languageEnglish
Pages (from-to)95-101
Number of pages7
JournalACS macro letters
Volume8
Issue number1
DOIs
Publication statusPublished - 15 Jan 2019

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Glucose
Polymers
Glycoconjugates
Carbohydrates
Nanoparticles
Imaging techniques
Methacrylates
Water
Cytotoxicity
Concanavalin A
Bioactivity
Macromolecules
Drug delivery
Sulfhydryl Compounds
Lectins
Ligands

Cite this

Kröger, A. P. P., Komil, M. I., Hamelmann, N. M., Juan, A., Stenzel, M. H., & Paulusse, J. M. J. (2019). Glucose Single-Chain Polymer Nanoparticles for Cellular Targeting. ACS macro letters, 8(1), 95-101. https://doi.org/10.1021/acsmacrolett.8b00812
Kröger, A. Pia P. ; Komil, Muhabbat I. ; Hamelmann, Naomi M. ; Juan, Alberto ; Stenzel, Martina H. ; Paulusse, Jos M.J. / Glucose Single-Chain Polymer Nanoparticles for Cellular Targeting. In: ACS macro letters. 2019 ; Vol. 8, No. 1. pp. 95-101.
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Kröger, APP, Komil, MI, Hamelmann, NM, Juan, A, Stenzel, MH & Paulusse, JMJ 2019, 'Glucose Single-Chain Polymer Nanoparticles for Cellular Targeting' ACS macro letters, vol. 8, no. 1, pp. 95-101. https://doi.org/10.1021/acsmacrolett.8b00812

Glucose Single-Chain Polymer Nanoparticles for Cellular Targeting. / Kröger, A. Pia P.; Komil, Muhabbat I.; Hamelmann, Naomi M.; Juan, Alberto; Stenzel, Martina H.; Paulusse, Jos M.J. (Corresponding Author).

In: ACS macro letters, Vol. 8, No. 1, 15.01.2019, p. 95-101.

Research output: Contribution to journalArticleAcademicpeer-review

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