Gold nanocrystal labeling allows low-density lipoprotein imaging from the subcellular to macroscopic level

Iris Eva Allijn; W. Leong; J. Tang; A. Gianella; A.J. Mieszawska; F. Fay; G. Ma; S. Russell; C.B. Callo; R.E. Gordon; E. Korkmaz; J.A. Post; Yihming Zhao; H. Gerritsen; Gerrit Storm; A. Thran; R. Proksa; H. Daerr; V. Fuster; E.A. Fisher; Z.A. Fayad; W.J.M. Mulder; D.P. Cormode

doi:10.1021/nn403258w

Gold nanocrystal labeling allows low-density lipoprotein imaging from the subcellular to macroscopic level

Iris Eva Allijn, W. Leong, J. Tang, A. Gianella, A.J. Mieszawska, F. Fay, G. Ma, S. Russell, C.B. Callo, R.E. Gordon, E. Korkmaz, J.A. Post, Yihming Zhao, H. Gerritsen, Gerrit Storm, A. Thran, R. Proksa, H. Daerr, V. Fuster, E.A. FisherZ.A. Fayad, W.J.M. Mulder, D.P. Cormode

Faculty of Science and Technology

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Low-density lipoprotein (LDL) plays a critical role in cholesterol transport and is closely linked to the progression of several diseases. This motivates the development of methods to study LDL behavior from the microscopic to whole-body level. We have developed an approach to efficiently load LDL with a range of diagnostically active nanocrystals or hydrophobic agents. We performed focused experiments on LDL labeled with gold nanocrystals (Au-LDL). The labeling procedure had minimal effect on LDL size, morphology, or composition. Biological function was found to be maintained from both in vitro and in vivo experiments. Tumor-bearing mice were injected intravenously with LDL, DiR-LDL, Au-LDL, or a gold-loaded nanoemulsion. LDL accumulation in the tumors was detected with whole-body imaging methods, such as computed tomography (CT), spectral CT, and fluorescence imaging. Cellular localization was studied with transmission electron microscopy and fluorescence techniques. This LDL labeling procedure should permit the study of lipoprotein biointeractions in unprecedented detail

Original language	Undefined
Pages (from-to)	9761-9670
Journal	ACS nano
Volume	7
Issue number	11
DOIs	https://doi.org/10.1021/nn403258w
Publication status	Published - 2013

Keywords

IR-90160
METIS-301797

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1021/nn403258w

Cite this

Allijn, I. E., Leong, W., Tang, J., Gianella, A., Mieszawska, A. J., Fay, F., Ma, G., Russell, S., Callo, C. B., Gordon, R. E., Korkmaz, E., Post, J. A., Zhao, Y., Gerritsen, H., Storm, G., Thran, A., Proksa, R., Daerr, H., Fuster, V., ... Cormode, D. P. (2013). Gold nanocrystal labeling allows low-density lipoprotein imaging from the subcellular to macroscopic level. ACS nano, 7(11), 9761-9670. https://doi.org/10.1021/nn403258w

@article{b66c2efebe9a496aaea5928be2ff76bb,

title = "Gold nanocrystal labeling allows low-density lipoprotein imaging from the subcellular to macroscopic level",

abstract = "Low-density lipoprotein (LDL) plays a critical role in cholesterol transport and is closely linked to the progression of several diseases. This motivates the development of methods to study LDL behavior from the microscopic to whole-body level. We have developed an approach to efficiently load LDL with a range of diagnostically active nanocrystals or hydrophobic agents. We performed focused experiments on LDL labeled with gold nanocrystals (Au-LDL). The labeling procedure had minimal effect on LDL size, morphology, or composition. Biological function was found to be maintained from both in vitro and in vivo experiments. Tumor-bearing mice were injected intravenously with LDL, DiR-LDL, Au-LDL, or a gold-loaded nanoemulsion. LDL accumulation in the tumors was detected with whole-body imaging methods, such as computed tomography (CT), spectral CT, and fluorescence imaging. Cellular localization was studied with transmission electron microscopy and fluorescence techniques. This LDL labeling procedure should permit the study of lipoprotein biointeractions in unprecedented detail",

keywords = "IR-90160, METIS-301797",

author = "Allijn, {Iris Eva} and W. Leong and J. Tang and A. Gianella and A.J. Mieszawska and F. Fay and G. Ma and S. Russell and C.B. Callo and R.E. Gordon and E. Korkmaz and J.A. Post and Yihming Zhao and H. Gerritsen and Gerrit Storm and A. Thran and R. Proksa and H. Daerr and V. Fuster and E.A. Fisher and Z.A. Fayad and W.J.M. Mulder and D.P. Cormode",

year = "2013",

doi = "10.1021/nn403258w",

language = "Undefined",

volume = "7",

pages = "9761--9670",

journal = "ACS nano",

issn = "1936-0851",

publisher = "American Chemical Society",

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Allijn, IE, Leong, W, Tang, J, Gianella, A, Mieszawska, AJ, Fay, F, Ma, G, Russell, S, Callo, CB, Gordon, RE, Korkmaz, E, Post, JA, Zhao, Y, Gerritsen, H, Storm, G, Thran, A, Proksa, R, Daerr, H, Fuster, V, Fisher, EA, Fayad, ZA, Mulder, WJM & Cormode, DP 2013, 'Gold nanocrystal labeling allows low-density lipoprotein imaging from the subcellular to macroscopic level', ACS nano, vol. 7, no. 11, pp. 9761-9670. https://doi.org/10.1021/nn403258w

TY - JOUR

T1 - Gold nanocrystal labeling allows low-density lipoprotein imaging from the subcellular to macroscopic level

AU - Allijn, Iris Eva

AU - Leong, W.

AU - Tang, J.

AU - Gianella, A.

AU - Mieszawska, A.J.

AU - Fay, F.

AU - Ma, G.

AU - Russell, S.

AU - Callo, C.B.

AU - Gordon, R.E.

AU - Korkmaz, E.

AU - Post, J.A.

AU - Zhao, Yihming

AU - Gerritsen, H.

AU - Storm, Gerrit

AU - Thran, A.

AU - Proksa, R.

AU - Daerr, H.

AU - Fuster, V.

AU - Fisher, E.A.

AU - Fayad, Z.A.

AU - Mulder, W.J.M.

AU - Cormode, D.P.

PY - 2013

Y1 - 2013

N2 - Low-density lipoprotein (LDL) plays a critical role in cholesterol transport and is closely linked to the progression of several diseases. This motivates the development of methods to study LDL behavior from the microscopic to whole-body level. We have developed an approach to efficiently load LDL with a range of diagnostically active nanocrystals or hydrophobic agents. We performed focused experiments on LDL labeled with gold nanocrystals (Au-LDL). The labeling procedure had minimal effect on LDL size, morphology, or composition. Biological function was found to be maintained from both in vitro and in vivo experiments. Tumor-bearing mice were injected intravenously with LDL, DiR-LDL, Au-LDL, or a gold-loaded nanoemulsion. LDL accumulation in the tumors was detected with whole-body imaging methods, such as computed tomography (CT), spectral CT, and fluorescence imaging. Cellular localization was studied with transmission electron microscopy and fluorescence techniques. This LDL labeling procedure should permit the study of lipoprotein biointeractions in unprecedented detail

AB - Low-density lipoprotein (LDL) plays a critical role in cholesterol transport and is closely linked to the progression of several diseases. This motivates the development of methods to study LDL behavior from the microscopic to whole-body level. We have developed an approach to efficiently load LDL with a range of diagnostically active nanocrystals or hydrophobic agents. We performed focused experiments on LDL labeled with gold nanocrystals (Au-LDL). The labeling procedure had minimal effect on LDL size, morphology, or composition. Biological function was found to be maintained from both in vitro and in vivo experiments. Tumor-bearing mice were injected intravenously with LDL, DiR-LDL, Au-LDL, or a gold-loaded nanoemulsion. LDL accumulation in the tumors was detected with whole-body imaging methods, such as computed tomography (CT), spectral CT, and fluorescence imaging. Cellular localization was studied with transmission electron microscopy and fluorescence techniques. This LDL labeling procedure should permit the study of lipoprotein biointeractions in unprecedented detail

KW - IR-90160

KW - METIS-301797

U2 - 10.1021/nn403258w

DO - 10.1021/nn403258w

M3 - Article

SN - 1936-0851

VL - 7

SP - 9761

EP - 9670

JO - ACS nano

JF - ACS nano

IS - 11

ER -