Gold Nanoparticle Assays: A New Golden Standard for Optical DNA Detection

Jeanne E. van Dongen; Hai  Le The; Ruben W. Kolkman; Dorothee  Wasserberg; Juriaan Huskens; Jan C.T. Eijkel; Loes Segerink

Gold Nanoparticle Assays: A New Golden Standard for Optical DNA Detection

Jeanne E. van Dongen, Hai Le The, Ruben W. Kolkman, Dorothee Wasserberg, Juriaan Huskens, Jan C.T. Eijkel, Loes Segerink

Research output: Contribution to conference › Poster › Academic

Abstract

Globally, it is estimated that cancer is responsible for 9.6 million deaths in 2018. For almost half of the cancer patients, the disease is diagnosed at an advanced stage, which can greatly reduce the chances of survival. The scope of this project is the detection of hypermethylated (hm) DNA in urine samples, with the potential of early stage and non-invasive detection of cancer. The typical concentration of tumor specific hmDNA in urine is between 10-12 - 10-18 M. Current available biochemical assays often lack the sensitivity and selectivity needed for sensing these specific sequences at such low concentrations. In this project gold nanoparticle assays are tested for detection of DNA fragments with single molecule sensitivity and the capability to detect sub-picomolar concentrations of oligonucleotides without the need of amplification steps.

Original language	English
Publication status	Published - 12 Dec 2019
Event	7th CHemistry As INnovating Science Conference, CHAINS 2019 - Veldhoven, Netherlands Duration: 10 Dec 2019 → 11 Dec 2019 Conference number: 7

Conference

Conference	7th CHemistry As INnovating Science Conference, CHAINS 2019
Abbreviated title	CHAINS 2019
Country/Territory	Netherlands
City	Veldhoven
Period	10/12/19 → 11/12/19

Cite this

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title = "Gold Nanoparticle Assays: A New Golden Standard for Optical DNA Detection",

abstract = "Globally, it is estimated that cancer is responsible for 9.6 million deaths in 2018. For almost half of the cancer patients, the disease is diagnosed at an advanced stage, which can greatly reduce the chances of survival. The scope of this project is the detection of hypermethylated (hm) DNA in urine samples, with the potential of early stage and non-invasive detection of cancer. The typical concentration of tumor specific hmDNA in urine is between 10-12 - 10-18 M. Current available biochemical assays often lack the sensitivity and selectivity needed for sensing these specific sequences at such low concentrations. In this project gold nanoparticle assays are tested for detection of DNA fragments with single molecule sensitivity and the capability to detect sub-picomolar concentrations of oligonucleotides without the need of amplification steps.",

author = "{van Dongen}, {Jeanne E.} and {Le The}, Hai and Kolkman, {Ruben W.} and Dorothee Wasserberg and Juriaan Huskens and Eijkel, {Jan C.T.} and Loes Segerink",

year = "2019",

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note = "7th CHemistry As INnovating Science Conference, CHAINS 2019, CHAINS 2019 ; Conference date: 10-12-2019 Through 11-12-2019",

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TY - CONF

T1 - Gold Nanoparticle Assays

T2 - 7th CHemistry As INnovating Science Conference, CHAINS 2019

AU - van Dongen, Jeanne E.

AU - Le The, Hai

AU - Kolkman, Ruben W.

AU - Wasserberg, Dorothee

AU - Huskens, Juriaan

AU - Eijkel, Jan C.T.

AU - Segerink, Loes

N1 - Conference code: 7

PY - 2019/12/12

Y1 - 2019/12/12

N2 - Globally, it is estimated that cancer is responsible for 9.6 million deaths in 2018. For almost half of the cancer patients, the disease is diagnosed at an advanced stage, which can greatly reduce the chances of survival. The scope of this project is the detection of hypermethylated (hm) DNA in urine samples, with the potential of early stage and non-invasive detection of cancer. The typical concentration of tumor specific hmDNA in urine is between 10-12 - 10-18 M. Current available biochemical assays often lack the sensitivity and selectivity needed for sensing these specific sequences at such low concentrations. In this project gold nanoparticle assays are tested for detection of DNA fragments with single molecule sensitivity and the capability to detect sub-picomolar concentrations of oligonucleotides without the need of amplification steps.

AB - Globally, it is estimated that cancer is responsible for 9.6 million deaths in 2018. For almost half of the cancer patients, the disease is diagnosed at an advanced stage, which can greatly reduce the chances of survival. The scope of this project is the detection of hypermethylated (hm) DNA in urine samples, with the potential of early stage and non-invasive detection of cancer. The typical concentration of tumor specific hmDNA in urine is between 10-12 - 10-18 M. Current available biochemical assays often lack the sensitivity and selectivity needed for sensing these specific sequences at such low concentrations. In this project gold nanoparticle assays are tested for detection of DNA fragments with single molecule sensitivity and the capability to detect sub-picomolar concentrations of oligonucleotides without the need of amplification steps.

M3 - Poster

Y2 - 10 December 2019 through 11 December 2019

ER -

Gold Nanoparticle Assays: A New Golden Standard for Optical DNA Detection

Abstract

Conference

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