Globally, it is estimated that cancer is responsible for 9.6 million deaths in 2018. For almost half of the cancer patients, the disease is diagnosed at an advanced stage, which can greatly reduce the chances of survival. The scope of this project is the detection of hypermethylated (hm) DNA in urine samples, with the potential of early stage and non-invasive detection of cancer. The typical concentration of tumor specific hmDNA in urine is between 10-12 - 10-18 M. Current available biochemical assays often lack the sensitivity and selectivity needed for sensing these specific sequences at such low concentrations. In this project gold nanoparticle assays are tested for detection of DNA fragments with single molecule sensitivity and the capability to detect sub-picomolar concentrations of oligonucleotides without the need of amplification steps.
|Publication status||Published - 12 Dec 2019|
|Event||7th CHemistry As INnovating Science Conference, CHAINS 2019 - Veldhoven, Netherlands|
Duration: 10 Dec 2019 → 11 Dec 2019
Conference number: 7
|Conference||7th CHemistry As INnovating Science Conference, CHAINS 2019|
|Abbreviated title||CHAINS 2019|
|Period||10/12/19 → 11/12/19|
van Dongen, J. E., Le The, H., Kolkman, R. W., Wasserberg, D., Huskens, J., Eijkel, J. C. T., & Segerink, L. (2019). Gold Nanoparticle Assays: A New Golden Standard for Optical DNA Detection. Poster session presented at 7th CHemistry As INnovating Science Conference, CHAINS 2019, Veldhoven, Netherlands.