TY - JOUR
T1 - Handheld versus mounted laser speckle contrast perfusion imaging demonstrated in psoriasis lesions
AU - Chizari, Ata
AU - Schaap, Mirjam J.
AU - Knop, Tom
AU - Boink, Yoeri E.
AU - Seyger, Marieke M.B.
AU - Steenbergen, Wiendelt
N1 - Funding Information:
This study was supported by the Open Technology program of the Netherlands Organization for Scientific Research (NWO), Domain Applied and Engineering Sciences, under Grant Number 14538. We thank Tjitske Bannink and Freek Bielevelt for their valuable help during implementation of the handheld laser speckle contrast imaging system. We are grateful to Beril Sirmacek for initializing the MATLAB R2019b (Mathworks)25 user-interface for data preview and acquisition.
Publisher Copyright:
© 2021, The Author(s).
Financial transaction number:
342134145
PY - 2021/12
Y1 - 2021/12
N2 - Enabling handheld perfusion imaging would drastically improve the feasibility of perfusion imaging in clinical practice. Therefore, we examine the performance of handheld laser speckle contrast imaging (LSCI) measurements compared to mounted measurements, demonstrated in psoriatic skin. A pipeline is introduced to process, analyze and compare data of 11 measurement pairs (mounted-handheld LSCI modes) operated on 5 patients and various skin locations. The on-surface speeds (i.e. speed of light beam movements on the surface) are quantified employing mean separation (MS) segmentation and enhanced correlation coefficient maximization (ECC). The average on-surface speeds are found to be 8.5 times greater in handheld mode compared to mounted mode. Frame alignment sharpens temporally averaged perfusion maps, especially in the handheld case. The results show that after proper post-processing, the handheld measurements are in agreement with the corresponding mounted measurements on a visual basis. The absolute movement-induced difference between mounted-handheld pairs after the background correction is 16.4±9.3% (mean ± std, n= 11), with an absolute median difference of 23.8 %. Realization of handheld LSCI facilitates measurements on a wide range of skin areas bringing more convenience for both patients and medical staff.
AB - Enabling handheld perfusion imaging would drastically improve the feasibility of perfusion imaging in clinical practice. Therefore, we examine the performance of handheld laser speckle contrast imaging (LSCI) measurements compared to mounted measurements, demonstrated in psoriatic skin. A pipeline is introduced to process, analyze and compare data of 11 measurement pairs (mounted-handheld LSCI modes) operated on 5 patients and various skin locations. The on-surface speeds (i.e. speed of light beam movements on the surface) are quantified employing mean separation (MS) segmentation and enhanced correlation coefficient maximization (ECC). The average on-surface speeds are found to be 8.5 times greater in handheld mode compared to mounted mode. Frame alignment sharpens temporally averaged perfusion maps, especially in the handheld case. The results show that after proper post-processing, the handheld measurements are in agreement with the corresponding mounted measurements on a visual basis. The absolute movement-induced difference between mounted-handheld pairs after the background correction is 16.4±9.3% (mean ± std, n= 11), with an absolute median difference of 23.8 %. Realization of handheld LSCI facilitates measurements on a wide range of skin areas bringing more convenience for both patients and medical staff.
KW - UT-Gold-D
UR - http://www.scopus.com/inward/record.url?scp=85113177869&partnerID=8YFLogxK
U2 - 10.1038/s41598-021-96218-6
DO - 10.1038/s41598-021-96218-6
M3 - Article
C2 - 34404886
AN - SCOPUS:85113177869
SN - 2045-2322
VL - 11
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 16646
ER -