High fidelity neuronal networks formed by plasma masking with a bilayer membrane: analysis of neurodegenerative and neuroprotective processes

Heike Hardelauf, Julia Sisnaiske, Amir Ali Taghipour-Anvari, Peter Jacob, Evelyn Drabiniok, Ulrich Marggraf, Jean-Philippe Frimat, Jan G. Hengstler, Andreas Neyer, Christoph van Thriel, Jonathan West

    Research output: Contribution to journalArticleAcademicpeer-review

    36 Citations (Scopus)

    Abstract

    Spatially defined neuronal networks have great potential to be used in a wide spectrum of neurobiology assays. We present an original technique for the precise and reproducible formation of neuronal networks. A PDMS membrane comprising through-holes aligned with interconnecting microchannels was used during oxygen plasma etching to dry mask a protein rejecting poly(ethylene glycol) (PEG) adlayer. Patterns were faithfully replicated to produce an oxidized interconnected array pattern which supported protein adsorption. Differentiated human SH-SY5Y neuron-like cells adhered to the array nodes with the micron-scale interconnecting tracks guiding neurite outgrowth to produce neuronal connections and establish a network. A 2.0 μm track width was optimal for high-level network formation and node compliance. These spatially standardized neuronal networks were used to analyse the dynamics of acrylamide-induced neurite degeneration and the protective effects of co-treatment with calpeptin or brain derived neurotrophic factor (BDNF).
    Original languageUndefined
    Pages (from-to)2763-2771
    Number of pages9
    JournalLab on a chip
    Volume11
    Issue number16
    DOIs
    Publication statusPublished - Jun 2011

    Keywords

    • METIS-281653
    • IR-78993
    • EWI-21019

    Cite this

    Hardelauf, H., Sisnaiske, J., Taghipour-Anvari, A. A., Jacob, P., Drabiniok, E., Marggraf, U., ... West, J. (2011). High fidelity neuronal networks formed by plasma masking with a bilayer membrane: analysis of neurodegenerative and neuroprotective processes. Lab on a chip, 11(16), 2763-2771. https://doi.org/10.1039/c1lc20257j