High-resolution electrophoretic separation and integrated-waveguide excitation of fluorescent DNA molecules in a lab on a chip

C. Dongre, Jasper van Weerd, G.A.J. Besselink, Rob van Weeghel, Rebecca Martinez Vazquez, Roberto Osellame, Giulio Cerullo, Marina Cretich, Marcella Chiari, Hugo Hoekstra, Markus Pollnau

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    16 Citations (Scopus)

    Abstract

    By applying integrated-waveguide laser excitation to an optofluidic chip, fluorescently labeled DNA molecules of 12 or 17 different sizes are separated by CE with high operating speed and low sample consumption of ~600 pL. When detecting the fluorescence signals of migrating DNA molecules with a PMT, the LOD is as low as 2.1 pM. In the diagnostically relevant size range (~150–1000 base-pairs) the molecules are separated with reproducibly high sizing accuracy (>99%) and the plug broadening follows Poissonian statistics. Variation of the power dependence of migration time on base-pair size – probably with temperature and condition of the sieving gel matrix – indicates that the capillary migration cannot be described by a simple physical law. Integrated-waveguide excitation of a 12-µm narrow microfluidic segment provides a spatio-temporal resolution that would, in principle, allow for a 20-fold better accuracy than the currently supported by state-of-the-art electrophoretic separation in microchips, thereby demonstrating the potential of this integrated optical approach to fulfill the resolution demands of future electrophoretic microchips.
    Original languageUndefined
    Article number10.1002/elps.201000126
    Pages (from-to)2584-2588
    Number of pages5
    JournalElectrophoresis
    Volume31
    Issue number15
    DOIs
    Publication statusPublished - Aug 2010

    Keywords

    • IOMS-SNS: SENSORS
    • EC Grant Agreement nr.: FP6/034562
    • EWI-18173
    • Fluorescence
    • IR-72446
    • Ultrasensitive
    • Lab on a chip
    • METIS-276063
    • Integrated optofluidics

    Cite this

    Dongre, C., van Weerd, J., Besselink, G. A. J., van Weeghel, R., Martinez Vazquez, R., Osellame, R., ... Pollnau, M. (2010). High-resolution electrophoretic separation and integrated-waveguide excitation of fluorescent DNA molecules in a lab on a chip. Electrophoresis, 31(15), 2584-2588. [10.1002/elps.201000126]. https://doi.org/10.1002/elps.201000126