High-resolution microcontact printing and transfer of massive arrays of microorganisms on planar and compartmentalized nanoporous aluminium oxide

Colin Ingham, Johan G. Bomer, A.J. Sprenkels, Albert van den Berg, Willem Vos, Johan van Hylckama Vlieg

    Research output: Contribution to journalArticleAcademicpeer-review

    24 Citations (Scopus)

    Abstract

    Handling microorganisms in high throughput and their deployment into miniaturized platforms presents significant challenges. Contact printing can be used to create dense arrays of viable microorganisms. Such "living arrays'', potentially with multiple identical replicates, are useful in the selection of improved industrial microorganisms, screening antimicrobials, clinical diagnostics, strain storage, and for research into microbial genetics. A high throughput method to print microorganisms at high density was devised, employing a microscope and a stamp with a massive array of PDMS pins. Viable bacteria (Lactobacillus plantarum, Esherichia coli), yeast (Candida albicans) and fungal spores (Aspergillus fumigatus) were deposited onto porous aluminium oxide (PAO) using arrays of pins with areas from 5 x 5 to 20 x 20 mu m. Printing onto PAO with up to 8100 pins of 20 x 20 mu m area with 3 replicates was achieved. Printing with up to 200 pins onto PAO culture chips (divided into 40 x 40 mu m culture areas) allowed inoculation followed by effective segregation of microcolonies during outgrowth. Additionally, it was possible to print mixtures of C. albicans and spores of A. fumigatus with a degree of selectivity by capture onto a chemically modified PAO surface. High resolution printing of microorganisms within segregated compartments and on functionalized PAO surfaces has significant advantages over what is possible on semi-solid surfaces such as agar.
    Original languageUndefined
    Pages (from-to)1410-1416
    Number of pages7
    JournalLab on a chip
    Volume10
    Issue number11
    DOIs
    Publication statusPublished - 2010

    Keywords

    • EWI-18131
    • IR-75108
    • METIS-276050

    Cite this

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    title = "High-resolution microcontact printing and transfer of massive arrays of microorganisms on planar and compartmentalized nanoporous aluminium oxide",
    abstract = "Handling microorganisms in high throughput and their deployment into miniaturized platforms presents significant challenges. Contact printing can be used to create dense arrays of viable microorganisms. Such {"}living arrays'', potentially with multiple identical replicates, are useful in the selection of improved industrial microorganisms, screening antimicrobials, clinical diagnostics, strain storage, and for research into microbial genetics. A high throughput method to print microorganisms at high density was devised, employing a microscope and a stamp with a massive array of PDMS pins. Viable bacteria (Lactobacillus plantarum, Esherichia coli), yeast (Candida albicans) and fungal spores (Aspergillus fumigatus) were deposited onto porous aluminium oxide (PAO) using arrays of pins with areas from 5 x 5 to 20 x 20 mu m. Printing onto PAO with up to 8100 pins of 20 x 20 mu m area with 3 replicates was achieved. Printing with up to 200 pins onto PAO culture chips (divided into 40 x 40 mu m culture areas) allowed inoculation followed by effective segregation of microcolonies during outgrowth. Additionally, it was possible to print mixtures of C. albicans and spores of A. fumigatus with a degree of selectivity by capture onto a chemically modified PAO surface. High resolution printing of microorganisms within segregated compartments and on functionalized PAO surfaces has significant advantages over what is possible on semi-solid surfaces such as agar.",
    keywords = "EWI-18131, IR-75108, METIS-276050",
    author = "Colin Ingham and Bomer, {Johan G.} and A.J. Sprenkels and {van den Berg}, Albert and Willem Vos and {van Hylckama Vlieg}, Johan",
    note = "10.1039/b925796a",
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    High-resolution microcontact printing and transfer of massive arrays of microorganisms on planar and compartmentalized nanoporous aluminium oxide. / Ingham, Colin; Bomer, Johan G.; Sprenkels, A.J.; van den Berg, Albert; Vos, Willem; van Hylckama Vlieg, Johan.

    In: Lab on a chip, Vol. 10, No. 11, 2010, p. 1410-1416.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - High-resolution microcontact printing and transfer of massive arrays of microorganisms on planar and compartmentalized nanoporous aluminium oxide

    AU - Ingham, Colin

    AU - Bomer, Johan G.

    AU - Sprenkels, A.J.

    AU - van den Berg, Albert

    AU - Vos, Willem

    AU - van Hylckama Vlieg, Johan

    N1 - 10.1039/b925796a

    PY - 2010

    Y1 - 2010

    N2 - Handling microorganisms in high throughput and their deployment into miniaturized platforms presents significant challenges. Contact printing can be used to create dense arrays of viable microorganisms. Such "living arrays'', potentially with multiple identical replicates, are useful in the selection of improved industrial microorganisms, screening antimicrobials, clinical diagnostics, strain storage, and for research into microbial genetics. A high throughput method to print microorganisms at high density was devised, employing a microscope and a stamp with a massive array of PDMS pins. Viable bacteria (Lactobacillus plantarum, Esherichia coli), yeast (Candida albicans) and fungal spores (Aspergillus fumigatus) were deposited onto porous aluminium oxide (PAO) using arrays of pins with areas from 5 x 5 to 20 x 20 mu m. Printing onto PAO with up to 8100 pins of 20 x 20 mu m area with 3 replicates was achieved. Printing with up to 200 pins onto PAO culture chips (divided into 40 x 40 mu m culture areas) allowed inoculation followed by effective segregation of microcolonies during outgrowth. Additionally, it was possible to print mixtures of C. albicans and spores of A. fumigatus with a degree of selectivity by capture onto a chemically modified PAO surface. High resolution printing of microorganisms within segregated compartments and on functionalized PAO surfaces has significant advantages over what is possible on semi-solid surfaces such as agar.

    AB - Handling microorganisms in high throughput and their deployment into miniaturized platforms presents significant challenges. Contact printing can be used to create dense arrays of viable microorganisms. Such "living arrays'', potentially with multiple identical replicates, are useful in the selection of improved industrial microorganisms, screening antimicrobials, clinical diagnostics, strain storage, and for research into microbial genetics. A high throughput method to print microorganisms at high density was devised, employing a microscope and a stamp with a massive array of PDMS pins. Viable bacteria (Lactobacillus plantarum, Esherichia coli), yeast (Candida albicans) and fungal spores (Aspergillus fumigatus) were deposited onto porous aluminium oxide (PAO) using arrays of pins with areas from 5 x 5 to 20 x 20 mu m. Printing onto PAO with up to 8100 pins of 20 x 20 mu m area with 3 replicates was achieved. Printing with up to 200 pins onto PAO culture chips (divided into 40 x 40 mu m culture areas) allowed inoculation followed by effective segregation of microcolonies during outgrowth. Additionally, it was possible to print mixtures of C. albicans and spores of A. fumigatus with a degree of selectivity by capture onto a chemically modified PAO surface. High resolution printing of microorganisms within segregated compartments and on functionalized PAO surfaces has significant advantages over what is possible on semi-solid surfaces such as agar.

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    KW - IR-75108

    KW - METIS-276050

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    DO - 10.1039/b925796a

    M3 - Article

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    ER -