High-resolution microcontact printing and transfer of massive arrays of microorganisms on planar and compartmentalized nanoporous aluminium oxide

Colin Ingham, Johan G. Bomer, A.J. Sprenkels, Albert van den Berg, Willem Vos, Johan van Hylckama Vlieg

Research output: Contribution to journalArticleAcademicpeer-review

23 Citations (Scopus)

Abstract

Handling microorganisms in high throughput and their deployment into miniaturized platforms presents significant challenges. Contact printing can be used to create dense arrays of viable microorganisms. Such "living arrays'', potentially with multiple identical replicates, are useful in the selection of improved industrial microorganisms, screening antimicrobials, clinical diagnostics, strain storage, and for research into microbial genetics. A high throughput method to print microorganisms at high density was devised, employing a microscope and a stamp with a massive array of PDMS pins. Viable bacteria (Lactobacillus plantarum, Esherichia coli), yeast (Candida albicans) and fungal spores (Aspergillus fumigatus) were deposited onto porous aluminium oxide (PAO) using arrays of pins with areas from 5 x 5 to 20 x 20 mu m. Printing onto PAO with up to 8100 pins of 20 x 20 mu m area with 3 replicates was achieved. Printing with up to 200 pins onto PAO culture chips (divided into 40 x 40 mu m culture areas) allowed inoculation followed by effective segregation of microcolonies during outgrowth. Additionally, it was possible to print mixtures of C. albicans and spores of A. fumigatus with a degree of selectivity by capture onto a chemically modified PAO surface. High resolution printing of microorganisms within segregated compartments and on functionalized PAO surfaces has significant advantages over what is possible on semi-solid surfaces such as agar.
Original languageUndefined
Pages (from-to)1410-1416
Number of pages7
JournalLab on a chip
Volume10
Issue number11
DOIs
Publication statusPublished - 2010

Keywords

  • EWI-18131
  • IR-75108
  • METIS-276050

Cite this

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title = "High-resolution microcontact printing and transfer of massive arrays of microorganisms on planar and compartmentalized nanoporous aluminium oxide",
abstract = "Handling microorganisms in high throughput and their deployment into miniaturized platforms presents significant challenges. Contact printing can be used to create dense arrays of viable microorganisms. Such {"}living arrays'', potentially with multiple identical replicates, are useful in the selection of improved industrial microorganisms, screening antimicrobials, clinical diagnostics, strain storage, and for research into microbial genetics. A high throughput method to print microorganisms at high density was devised, employing a microscope and a stamp with a massive array of PDMS pins. Viable bacteria (Lactobacillus plantarum, Esherichia coli), yeast (Candida albicans) and fungal spores (Aspergillus fumigatus) were deposited onto porous aluminium oxide (PAO) using arrays of pins with areas from 5 x 5 to 20 x 20 mu m. Printing onto PAO with up to 8100 pins of 20 x 20 mu m area with 3 replicates was achieved. Printing with up to 200 pins onto PAO culture chips (divided into 40 x 40 mu m culture areas) allowed inoculation followed by effective segregation of microcolonies during outgrowth. Additionally, it was possible to print mixtures of C. albicans and spores of A. fumigatus with a degree of selectivity by capture onto a chemically modified PAO surface. High resolution printing of microorganisms within segregated compartments and on functionalized PAO surfaces has significant advantages over what is possible on semi-solid surfaces such as agar.",
keywords = "EWI-18131, IR-75108, METIS-276050",
author = "Colin Ingham and Bomer, {Johan G.} and A.J. Sprenkels and {van den Berg}, Albert and Willem Vos and {van Hylckama Vlieg}, Johan",
note = "10.1039/b925796a",
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volume = "10",
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publisher = "Royal Society of Chemistry",
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High-resolution microcontact printing and transfer of massive arrays of microorganisms on planar and compartmentalized nanoporous aluminium oxide. / Ingham, Colin; Bomer, Johan G.; Sprenkels, A.J.; van den Berg, Albert; Vos, Willem; van Hylckama Vlieg, Johan.

In: Lab on a chip, Vol. 10, No. 11, 2010, p. 1410-1416.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - High-resolution microcontact printing and transfer of massive arrays of microorganisms on planar and compartmentalized nanoporous aluminium oxide

AU - Ingham, Colin

AU - Bomer, Johan G.

AU - Sprenkels, A.J.

AU - van den Berg, Albert

AU - Vos, Willem

AU - van Hylckama Vlieg, Johan

N1 - 10.1039/b925796a

PY - 2010

Y1 - 2010

N2 - Handling microorganisms in high throughput and their deployment into miniaturized platforms presents significant challenges. Contact printing can be used to create dense arrays of viable microorganisms. Such "living arrays'', potentially with multiple identical replicates, are useful in the selection of improved industrial microorganisms, screening antimicrobials, clinical diagnostics, strain storage, and for research into microbial genetics. A high throughput method to print microorganisms at high density was devised, employing a microscope and a stamp with a massive array of PDMS pins. Viable bacteria (Lactobacillus plantarum, Esherichia coli), yeast (Candida albicans) and fungal spores (Aspergillus fumigatus) were deposited onto porous aluminium oxide (PAO) using arrays of pins with areas from 5 x 5 to 20 x 20 mu m. Printing onto PAO with up to 8100 pins of 20 x 20 mu m area with 3 replicates was achieved. Printing with up to 200 pins onto PAO culture chips (divided into 40 x 40 mu m culture areas) allowed inoculation followed by effective segregation of microcolonies during outgrowth. Additionally, it was possible to print mixtures of C. albicans and spores of A. fumigatus with a degree of selectivity by capture onto a chemically modified PAO surface. High resolution printing of microorganisms within segregated compartments and on functionalized PAO surfaces has significant advantages over what is possible on semi-solid surfaces such as agar.

AB - Handling microorganisms in high throughput and their deployment into miniaturized platforms presents significant challenges. Contact printing can be used to create dense arrays of viable microorganisms. Such "living arrays'', potentially with multiple identical replicates, are useful in the selection of improved industrial microorganisms, screening antimicrobials, clinical diagnostics, strain storage, and for research into microbial genetics. A high throughput method to print microorganisms at high density was devised, employing a microscope and a stamp with a massive array of PDMS pins. Viable bacteria (Lactobacillus plantarum, Esherichia coli), yeast (Candida albicans) and fungal spores (Aspergillus fumigatus) were deposited onto porous aluminium oxide (PAO) using arrays of pins with areas from 5 x 5 to 20 x 20 mu m. Printing onto PAO with up to 8100 pins of 20 x 20 mu m area with 3 replicates was achieved. Printing with up to 200 pins onto PAO culture chips (divided into 40 x 40 mu m culture areas) allowed inoculation followed by effective segregation of microcolonies during outgrowth. Additionally, it was possible to print mixtures of C. albicans and spores of A. fumigatus with a degree of selectivity by capture onto a chemically modified PAO surface. High resolution printing of microorganisms within segregated compartments and on functionalized PAO surfaces has significant advantages over what is possible on semi-solid surfaces such as agar.

KW - EWI-18131

KW - IR-75108

KW - METIS-276050

U2 - 10.1039/b925796a

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M3 - Article

VL - 10

SP - 1410

EP - 1416

JO - Lab on a chip

JF - Lab on a chip

SN - 1473-0197

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