Abstract
This study demonstrates the use of degenerate primers in combination with CRISPR-based detection for high selectivity measurements of high-risk HPV types. We found that the E6 genes are more suitable for this purpose than the L1 gene, and the lbCas12a performs better than AsCas12a in differentiating between HPV types. Initial steps toward integrating this method into an easy-to-use device have been taken, with future research focusing on incorporating the entire process, including DNA extraction, target amplification, and target detection, into a single device. This advancement aims to make primary HPV screening feasible in low-resource settings, improving accessibility and early detection capabilities.
| Original language | English |
|---|---|
| Title of host publication | 2024 IEEE Sensors, SENSORS 2024 - Conference Proceedings |
| Place of Publication | Piscataway, NJ |
| Publisher | IEEE |
| Number of pages | 4 |
| ISBN (Electronic) | 979-8-3503-6351-7 |
| ISBN (Print) | 979-8-3503-6352-4 |
| DOIs | |
| Publication status | Published - 17 Dec 2024 |
| Event | IEEE SENSORS 2024 - Kobe Portopia Hotel, Kobe, Japan Duration: 20 Oct 2024 → 23 Oct 2024 https://2024.ieee-sensorsconference.org/ |
Publication series
| Name | Proceedings of IEEE Sensors |
|---|---|
| Publisher | IEEE |
| Volume | 2024 |
| ISSN (Print) | 1930-0395 |
| ISSN (Electronic) | 2168-9229 |
Conference
| Conference | IEEE SENSORS 2024 |
|---|---|
| Country/Territory | Japan |
| City | Kobe |
| Period | 20/10/24 → 23/10/24 |
| Internet address |
Keywords
- 2025 OA procedure
- CRISPRICas
- HPV
- Multiplexing
- Biosensor