High-throughput and multiplexed regeneration buffer scouting for affinity-based interactions

Karin P.M. Geuijen, Richardus B.M. Schasfoort, Rene H. Wijffels, Michel H.M. Eppink

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Affinity-based analyses on biosensors depend partly on regeneration between measurements. Regeneration is performed with a buffer that efficiently breaks all interactions between ligand and analyte while maintaining the active binding site of the ligand. We demonstrated a regeneration buffer scouting using the combination of a continuous flow microspotter with a surface plasmon resonance imaging platform to simultaneously test 48 different regeneration buffers on a single biosensor. Optimal regeneration conditions are found within hours and consume little amounts of buffers, analyte, and ligand. This workflow can be applied to any ligand that is coupled through amine, thiol, or streptavidin immobilization.
Original languageEnglish
Pages (from-to)38-40
JournalAnalytical biochemistry
Volume454
DOIs
Publication statusPublished - 2014

Fingerprint

Regeneration
Buffers
Throughput
Ligands
Biosensors
Biosensing Techniques
Streptavidin
Surface plasmon resonance
Sulfhydryl Compounds
Surface Plasmon Resonance
Workflow
Amines
Immobilization
Binding Sites
Catalytic Domain
Imaging techniques

Keywords

  • IR-97041
  • METIS-311459

Cite this

Geuijen, Karin P.M. ; Schasfoort, Richardus B.M. ; Wijffels, Rene H. ; Eppink, Michel H.M. / High-throughput and multiplexed regeneration buffer scouting for affinity-based interactions. In: Analytical biochemistry. 2014 ; Vol. 454. pp. 38-40.
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High-throughput and multiplexed regeneration buffer scouting for affinity-based interactions. / Geuijen, Karin P.M.; Schasfoort, Richardus B.M.; Wijffels, Rene H.; Eppink, Michel H.M.

In: Analytical biochemistry, Vol. 454, 2014, p. 38-40.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - High-throughput and multiplexed regeneration buffer scouting for affinity-based interactions

AU - Geuijen, Karin P.M.

AU - Schasfoort, Richardus B.M.

AU - Wijffels, Rene H.

AU - Eppink, Michel H.M.

PY - 2014

Y1 - 2014

N2 - Affinity-based analyses on biosensors depend partly on regeneration between measurements. Regeneration is performed with a buffer that efficiently breaks all interactions between ligand and analyte while maintaining the active binding site of the ligand. We demonstrated a regeneration buffer scouting using the combination of a continuous flow microspotter with a surface plasmon resonance imaging platform to simultaneously test 48 different regeneration buffers on a single biosensor. Optimal regeneration conditions are found within hours and consume little amounts of buffers, analyte, and ligand. This workflow can be applied to any ligand that is coupled through amine, thiol, or streptavidin immobilization.

AB - Affinity-based analyses on biosensors depend partly on regeneration between measurements. Regeneration is performed with a buffer that efficiently breaks all interactions between ligand and analyte while maintaining the active binding site of the ligand. We demonstrated a regeneration buffer scouting using the combination of a continuous flow microspotter with a surface plasmon resonance imaging platform to simultaneously test 48 different regeneration buffers on a single biosensor. Optimal regeneration conditions are found within hours and consume little amounts of buffers, analyte, and ligand. This workflow can be applied to any ligand that is coupled through amine, thiol, or streptavidin immobilization.

KW - IR-97041

KW - METIS-311459

U2 - 10.1016/j.ab.2014.03.011

DO - 10.1016/j.ab.2014.03.011

M3 - Article

VL - 454

SP - 38

EP - 40

JO - Analytical biochemistry

JF - Analytical biochemistry

SN - 0003-2697

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