High-throughput approaches for screening and analysis of cell behaviors

Jungmok Seo, Jung Youn Shin, Jeroen Leijten, Oju Jeon, Gulden Camci-Unal, Anna D. Dikina, Katelyn Brinegar, Amir M. Ghaemmaghami, Eben Alsberg (Corresponding Author), Ali Khademhosseini*

*Corresponding author for this work

Research output: Contribution to journalReview articleAcademicpeer-review

21 Citations (Scopus)
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Abstract

The rapid development of new biomaterials and techniques to modify them challenge our capability to characterize them using conventional methods. In response, numerous high-throughput (HT) strategies are being developed to analyze biomaterials and their interactions with cells using combinatorial approaches. Moreover, these systematic analyses have the power to uncover effects of delivered soluble bioactive molecules on cell responses. In this review, we describe the recent developments in HT approaches that help identify cellular microenvironments affecting cell behaviors and highlight HT screening of biochemical libraries for gene delivery, drug discovery, and toxicological studies. We also discuss HT techniques for the analyses of cell secreted biomolecules and provide perspectives on the future utility of HT approaches in biomedical engineering.

Original languageEnglish
Pages (from-to)85-101
Number of pages17
JournalBiomaterials
Volume153
Early online date21 Jun 2017
DOIs
Publication statusPublished - 1 Jan 2018

Keywords

  • Biomaterial screening
  • Biomolecule delivery
  • Cell-biomaterial interactions
  • Cellular microenvironments
  • High-throughput biosensor
  • High-throughput system

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    Seo, J., Shin, J. Y., Leijten, J., Jeon, O., Camci-Unal, G., Dikina, A. D., ... Khademhosseini, A. (2018). High-throughput approaches for screening and analysis of cell behaviors. Biomaterials, 153, 85-101. https://doi.org/10.1016/j.biomaterials.2017.06.022