Abstract
In this article, we present a microfluidic device capable of successive high-yield single-cell encapsulation in droplets, with additional droplet pairing, fusion, and shrinkage. Deterministic single-cell encapsulation is realized using Dean-coupled inertial ordering of cells in a Yin-Yang-shaped curved microchannel using a double T-junction, with a frequency over 2000 Hz, followed by controlled droplet pairing with a 100% success rate. Subsequently, droplet fusion is realized using electrical actuation resulting in electro-coalescence of two droplets, each containing a single HL60 cell, with 95% efficiency. Finally, volume reduction of the fused droplet up to 75% is achieved by a triple pitchfork structure. This droplet volume reduction is necessary to obtain close cell-cell membrane contact necessary for final cell electrofusion, leading to hybridoma formation, which is the ultimate aim of this research
Original language | Undefined |
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Pages (from-to) | 385-392 |
Number of pages | 8 |
Journal | Electrophoresis |
Volume | 34 |
Issue number | 23 |
DOIs | |
Publication status | Published - Feb 2014 |
Keywords
- Micro-fluidics
- IR-88248
- METIS-300217
- High throughput
- EWI-24098
- Deterministic single-cell encapsulation
- Droplets
- Pairing