High-throughput on-chip DNA fragmentation

Lingling Shui, Mingliang Jin, Johan G. Bomer, Edwin Carlen, Albert van den Berg

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademic

19 Downloads (Pure)

Abstract

free microfluidic deoxyribonucleic acid (DNA) fragmentation chip that is based on hydrodynamic shearing. Genomic DNA has been reproducibly fragmented with 2-10 kbp fragment lengths by applying hydraulic pressure ΔP across micromachined constrictions in the microfluidic channels. The utilization of a series of constrictions reduces the variance of the fragmented DNA length distribution; and parallel microfluidic channels design eliminates the device clogging.
Original languageUndefined
Title of host publication21st Micromechanics and Micro systems Europe Workshop, MME 2010
EditorsLeon Abelmann, L. Abelmann, J.P.J. Groenland, J.W. van Honschoten
Place of PublicationEnschede
PublisherTwente University Press (TUP)
Pages124-127
Number of pages4
ISBN (Print)978-90-81673716
Publication statusPublished - 2010
Event21st Micromechanics and Microsystems Europe Workshop, MME 2010 - University of Twente, Enschede, Netherlands
Duration: 26 Sep 201029 Sep 2010
Conference number: 21

Publication series

Name
PublisherTwente University Press

Workshop

Workshop21st Micromechanics and Microsystems Europe Workshop, MME 2010
Abbreviated titleMME
CountryNetherlands
CityEnschede
Period26/09/1029/09/10

Keywords

  • METIS-277553
  • IR-76045
  • Micro-fluidics
  • EWI-19641
  • Hydrodynamic shearing
  • BIOS-Nanosensors
  • DNA fragmentation

Cite this

Shui, L., Jin, M., Bomer, J. G., Carlen, E., & van den Berg, A. (2010). High-throughput on-chip DNA fragmentation. In L. Abelmann, L. Abelmann, J. P. J. Groenland, & J. W. van Honschoten (Eds.), 21st Micromechanics and Micro systems Europe Workshop, MME 2010 (pp. 124-127). Enschede: Twente University Press (TUP).
Shui, Lingling ; Jin, Mingliang ; Bomer, Johan G. ; Carlen, Edwin ; van den Berg, Albert. / High-throughput on-chip DNA fragmentation. 21st Micromechanics and Micro systems Europe Workshop, MME 2010. editor / Leon Abelmann ; L. Abelmann ; J.P.J. Groenland ; J.W. van Honschoten. Enschede : Twente University Press (TUP), 2010. pp. 124-127
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keywords = "METIS-277553, IR-76045, Micro-fluidics, EWI-19641, Hydrodynamic shearing, BIOS-Nanosensors, DNA fragmentation",
author = "Lingling Shui and Mingliang Jin and Bomer, {Johan G.} and Edwin Carlen and {van den Berg}, Albert",
year = "2010",
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publisher = "Twente University Press (TUP)",
pages = "124--127",
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Shui, L, Jin, M, Bomer, JG, Carlen, E & van den Berg, A 2010, High-throughput on-chip DNA fragmentation. in L Abelmann, L Abelmann, JPJ Groenland & JW van Honschoten (eds), 21st Micromechanics and Micro systems Europe Workshop, MME 2010. Twente University Press (TUP), Enschede, pp. 124-127, 21st Micromechanics and Microsystems Europe Workshop, MME 2010, Enschede, Netherlands, 26/09/10.

High-throughput on-chip DNA fragmentation. / Shui, Lingling; Jin, Mingliang; Bomer, Johan G.; Carlen, Edwin; van den Berg, Albert.

21st Micromechanics and Micro systems Europe Workshop, MME 2010. ed. / Leon Abelmann; L. Abelmann; J.P.J. Groenland; J.W. van Honschoten. Enschede : Twente University Press (TUP), 2010. p. 124-127.

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademic

TY - GEN

T1 - High-throughput on-chip DNA fragmentation

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AU - Jin, Mingliang

AU - Bomer, Johan G.

AU - Carlen, Edwin

AU - van den Berg, Albert

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N2 - free microfluidic deoxyribonucleic acid (DNA) fragmentation chip that is based on hydrodynamic shearing. Genomic DNA has been reproducibly fragmented with 2-10 kbp fragment lengths by applying hydraulic pressure ΔP across micromachined constrictions in the microfluidic channels. The utilization of a series of constrictions reduces the variance of the fragmented DNA length distribution; and parallel microfluidic channels design eliminates the device clogging.

AB - free microfluidic deoxyribonucleic acid (DNA) fragmentation chip that is based on hydrodynamic shearing. Genomic DNA has been reproducibly fragmented with 2-10 kbp fragment lengths by applying hydraulic pressure ΔP across micromachined constrictions in the microfluidic channels. The utilization of a series of constrictions reduces the variance of the fragmented DNA length distribution; and parallel microfluidic channels design eliminates the device clogging.

KW - METIS-277553

KW - IR-76045

KW - Micro-fluidics

KW - EWI-19641

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KW - BIOS-Nanosensors

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BT - 21st Micromechanics and Micro systems Europe Workshop, MME 2010

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A2 - Groenland, J.P.J.

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Shui L, Jin M, Bomer JG, Carlen E, van den Berg A. High-throughput on-chip DNA fragmentation. In Abelmann L, Abelmann L, Groenland JPJ, van Honschoten JW, editors, 21st Micromechanics and Micro systems Europe Workshop, MME 2010. Enschede: Twente University Press (TUP). 2010. p. 124-127