High-throughput on-chip DNA fragmentation

Lingling Shui, Mingliang Jin, Johan G. Bomer, Edwin Carlen, Albert van den Berg

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademic

    22 Downloads (Pure)

    Abstract

    free microfluidic deoxyribonucleic acid (DNA) fragmentation chip that is based on hydrodynamic shearing. Genomic DNA has been reproducibly fragmented with 2-10 kbp fragment lengths by applying hydraulic pressure ΔP across micromachined constrictions in the microfluidic channels. The utilization of a series of constrictions reduces the variance of the fragmented DNA length distribution; and parallel microfluidic channels design eliminates the device clogging.
    Original languageUndefined
    Title of host publication21st Micromechanics and Micro systems Europe Workshop, MME 2010
    EditorsLeon Abelmann, L. Abelmann, J.P.J. Groenland, J.W. van Honschoten
    Place of PublicationEnschede
    PublisherTwente University Press (TUP)
    Pages124-127
    Number of pages4
    ISBN (Print)978-90-81673716
    Publication statusPublished - 2010
    Event21st Micromechanics and Microsystems Europe Workshop, MME 2010 - University of Twente, Enschede, Netherlands
    Duration: 26 Sep 201029 Sep 2010
    Conference number: 21

    Publication series

    Name
    PublisherTwente University Press

    Workshop

    Workshop21st Micromechanics and Microsystems Europe Workshop, MME 2010
    Abbreviated titleMME
    CountryNetherlands
    CityEnschede
    Period26/09/1029/09/10

    Keywords

    • METIS-277553
    • IR-76045
    • Micro-fluidics
    • EWI-19641
    • Hydrodynamic shearing
    • BIOS-Nanosensors
    • DNA fragmentation

    Cite this

    Shui, L., Jin, M., Bomer, J. G., Carlen, E., & van den Berg, A. (2010). High-throughput on-chip DNA fragmentation. In L. Abelmann, L. Abelmann, J. P. J. Groenland, & J. W. van Honschoten (Eds.), 21st Micromechanics and Micro systems Europe Workshop, MME 2010 (pp. 124-127). Enschede: Twente University Press (TUP).
    Shui, Lingling ; Jin, Mingliang ; Bomer, Johan G. ; Carlen, Edwin ; van den Berg, Albert. / High-throughput on-chip DNA fragmentation. 21st Micromechanics and Micro systems Europe Workshop, MME 2010. editor / Leon Abelmann ; L. Abelmann ; J.P.J. Groenland ; J.W. van Honschoten. Enschede : Twente University Press (TUP), 2010. pp. 124-127
    @inproceedings{4c202d2caaf34a71916676890fec1bc6,
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    abstract = "free microfluidic deoxyribonucleic acid (DNA) fragmentation chip that is based on hydrodynamic shearing. Genomic DNA has been reproducibly fragmented with 2-10 kbp fragment lengths by applying hydraulic pressure ΔP across micromachined constrictions in the microfluidic channels. The utilization of a series of constrictions reduces the variance of the fragmented DNA length distribution; and parallel microfluidic channels design eliminates the device clogging.",
    keywords = "METIS-277553, IR-76045, Micro-fluidics, EWI-19641, Hydrodynamic shearing, BIOS-Nanosensors, DNA fragmentation",
    author = "Lingling Shui and Mingliang Jin and Bomer, {Johan G.} and Edwin Carlen and {van den Berg}, Albert",
    year = "2010",
    language = "Undefined",
    isbn = "978-90-81673716",
    publisher = "Twente University Press (TUP)",
    pages = "124--127",
    editor = "Leon Abelmann and L. Abelmann and J.P.J. Groenland and {van Honschoten}, J.W.",
    booktitle = "21st Micromechanics and Micro systems Europe Workshop, MME 2010",
    address = "Netherlands",

    }

    Shui, L, Jin, M, Bomer, JG, Carlen, E & van den Berg, A 2010, High-throughput on-chip DNA fragmentation. in L Abelmann, L Abelmann, JPJ Groenland & JW van Honschoten (eds), 21st Micromechanics and Micro systems Europe Workshop, MME 2010. Twente University Press (TUP), Enschede, pp. 124-127, 21st Micromechanics and Microsystems Europe Workshop, MME 2010, Enschede, Netherlands, 26/09/10.

    High-throughput on-chip DNA fragmentation. / Shui, Lingling; Jin, Mingliang; Bomer, Johan G.; Carlen, Edwin; van den Berg, Albert.

    21st Micromechanics and Micro systems Europe Workshop, MME 2010. ed. / Leon Abelmann; L. Abelmann; J.P.J. Groenland; J.W. van Honschoten. Enschede : Twente University Press (TUP), 2010. p. 124-127.

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademic

    TY - GEN

    T1 - High-throughput on-chip DNA fragmentation

    AU - Shui, Lingling

    AU - Jin, Mingliang

    AU - Bomer, Johan G.

    AU - Carlen, Edwin

    AU - van den Berg, Albert

    PY - 2010

    Y1 - 2010

    N2 - free microfluidic deoxyribonucleic acid (DNA) fragmentation chip that is based on hydrodynamic shearing. Genomic DNA has been reproducibly fragmented with 2-10 kbp fragment lengths by applying hydraulic pressure ΔP across micromachined constrictions in the microfluidic channels. The utilization of a series of constrictions reduces the variance of the fragmented DNA length distribution; and parallel microfluidic channels design eliminates the device clogging.

    AB - free microfluidic deoxyribonucleic acid (DNA) fragmentation chip that is based on hydrodynamic shearing. Genomic DNA has been reproducibly fragmented with 2-10 kbp fragment lengths by applying hydraulic pressure ΔP across micromachined constrictions in the microfluidic channels. The utilization of a series of constrictions reduces the variance of the fragmented DNA length distribution; and parallel microfluidic channels design eliminates the device clogging.

    KW - METIS-277553

    KW - IR-76045

    KW - Micro-fluidics

    KW - EWI-19641

    KW - Hydrodynamic shearing

    KW - BIOS-Nanosensors

    KW - DNA fragmentation

    M3 - Conference contribution

    SN - 978-90-81673716

    SP - 124

    EP - 127

    BT - 21st Micromechanics and Micro systems Europe Workshop, MME 2010

    A2 - Abelmann, Leon

    A2 - Abelmann, L.

    A2 - Groenland, J.P.J.

    A2 - van Honschoten, J.W.

    PB - Twente University Press (TUP)

    CY - Enschede

    ER -

    Shui L, Jin M, Bomer JG, Carlen E, van den Berg A. High-throughput on-chip DNA fragmentation. In Abelmann L, Abelmann L, Groenland JPJ, van Honschoten JW, editors, 21st Micromechanics and Micro systems Europe Workshop, MME 2010. Enschede: Twente University Press (TUP). 2010. p. 124-127