High-throughput on-chip DNA fragmentation

Lingling Shui, Mingliang Jin, Johan G. Bomer, Edwin Carlen, Albert van den Berg

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademic

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    Abstract

    free microfluidic deoxyribonucleic acid (DNA) fragmentation chip that is based on hydrodynamic shearing. Genomic DNA has been reproducibly fragmented with 2-10 kbp fragment lengths by applying hydraulic pressure ΔP across micromachined constrictions in the microfluidic channels. The utilization of a series of constrictions reduces the variance of the fragmented DNA length distribution; and parallel microfluidic channels design eliminates the device clogging.
    Original languageUndefined
    Title of host publication21st Micromechanics and Micro systems Europe Workshop, MME 2010
    EditorsLeon Abelmann, L. Abelmann, J.P.J. Groenland, J.W. van Honschoten
    Place of PublicationEnschede
    PublisherTwente University Press (TUP)
    Pages124-127
    Number of pages4
    ISBN (Print)978-90-81673716
    Publication statusPublished - 2010
    Event21st Micromechanics and Microsystems Europe Workshop, MME 2010 - University of Twente, Enschede, Netherlands
    Duration: 26 Sep 201029 Sep 2010
    Conference number: 21

    Publication series

    Name
    PublisherTwente University Press

    Workshop

    Workshop21st Micromechanics and Microsystems Europe Workshop, MME 2010
    Abbreviated titleMME
    CountryNetherlands
    CityEnschede
    Period26/09/1029/09/10

    Keywords

    • METIS-277553
    • IR-76045
    • Micro-fluidics
    • EWI-19641
    • Hydrodynamic shearing
    • BIOS-Nanosensors
    • DNA fragmentation

    Cite this

    Shui, L., Jin, M., Bomer, J. G., Carlen, E., & van den Berg, A. (2010). High-throughput on-chip DNA fragmentation. In L. Abelmann, L. Abelmann, J. P. J. Groenland, & J. W. van Honschoten (Eds.), 21st Micromechanics and Micro systems Europe Workshop, MME 2010 (pp. 124-127). Enschede: Twente University Press (TUP).