High-throughput on-chip DNA fragmentation

Lingling Shui, Mingliang Jin, Johan G. Bomer, Edwin Carlen, Albert van den Berg

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademic

    60 Downloads (Pure)


    free microfluidic deoxyribonucleic acid (DNA) fragmentation chip that is based on hydrodynamic shearing. Genomic DNA has been reproducibly fragmented with 2-10 kbp fragment lengths by applying hydraulic pressure ΔP across micromachined constrictions in the microfluidic channels. The utilization of a series of constrictions reduces the variance of the fragmented DNA length distribution; and parallel microfluidic channels design eliminates the device clogging.
    Original languageUndefined
    Title of host publication21st Micromechanics and Micro systems Europe Workshop, MME 2010
    EditorsLeon Abelmann, L. Abelmann, J.P.J. Groenland, J.W. van Honschoten
    Place of PublicationEnschede
    PublisherTwente University Press (TUP)
    Number of pages4
    ISBN (Print)978-90-81673716
    Publication statusPublished - 2010
    Event21st Micromechanics and Microsystems Europe Workshop, MME 2010 - University of Twente, Enschede, Netherlands
    Duration: 26 Sept 201029 Sept 2010
    Conference number: 21

    Publication series

    PublisherTwente University Press


    Workshop21st Micromechanics and Microsystems Europe Workshop, MME 2010
    Abbreviated titleMME


    • METIS-277553
    • IR-76045
    • Micro-fluidics
    • EWI-19641
    • Hydrodynamic shearing
    • BIOS-Nanosensors
    • DNA fragmentation

    Cite this