High-throughput on-chip DNA fragmentation

Lingling Shui; Mingliang Jin; Johan G. Bomer; Edwin Carlen; Albert van den Berg

High-throughput on-chip DNA fragmentation

Lingling Shui
, Mingliang Jin
, Johan G. Bomer
, Edwin Carlen
, Albert van den Berg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic

117 Downloads (Pure)

Abstract

free microfluidic deoxyribonucleic acid (DNA) fragmentation chip that is based on hydrodynamic shearing. Genomic DNA has been reproducibly fragmented with 2-10 kbp fragment lengths by applying hydraulic pressure ΔP across micromachined constrictions in the microfluidic channels. The utilization of a series of constrictions reduces the variance of the fragmented DNA length distribution; and parallel microfluidic channels design eliminates the device clogging.

Original language	Undefined
Title of host publication	21st Micromechanics and Micro systems Europe Workshop, MME 2010
Editors	Leon Abelmann, L. Abelmann, J.P.J. Groenland, J.W. van Honschoten
Place of Publication	Enschede
Publisher	Twente University Press (TUP)
Pages	124-127
Number of pages	4
ISBN (Print)	978-90-81673716
Publication status	Published - 2010
Event	21st Micromechanics and Microsystems Europe Workshop, MME 2010 - University of Twente, Enschede, Netherlands Duration: 26 Sept 2010 → 29 Sept 2010 Conference number: 21

Publication series

Name
Publisher	Twente University Press

Workshop

Workshop	21st Micromechanics and Microsystems Europe Workshop, MME 2010
Abbreviated title	MME
Country/Territory	Netherlands
City	Enschede
Period	26/09/10 → 29/09/10

Keywords

METIS-277553
IR-76045
Micro-fluidics
EWI-19641
Hydrodynamic shearing
BIOS-Nanosensors
DNA fragmentation

Access to Document

Cite this

@inproceedings{4c202d2caaf34a71916676890fec1bc6,

title = "High-throughput on-chip DNA fragmentation",

abstract = "free microfluidic deoxyribonucleic acid (DNA) fragmentation chip that is based on hydrodynamic shearing. Genomic DNA has been reproducibly fragmented with 2-10 kbp fragment lengths by applying hydraulic pressure ΔP across micromachined constrictions in the microfluidic channels. The utilization of a series of constrictions reduces the variance of the fragmented DNA length distribution; and parallel microfluidic channels design eliminates the device clogging.",

keywords = "METIS-277553, IR-76045, Micro-fluidics, EWI-19641, Hydrodynamic shearing, BIOS-Nanosensors, DNA fragmentation",

author = "Lingling Shui and Mingliang Jin and Bomer, \{Johan G.\} and Edwin Carlen and \{van den Berg\}, Albert",

year = "2010",

language = "Undefined",

isbn = "978-90-81673716",

publisher = "Twente University Press (TUP)",

pages = "124--127",

editor = "Leon Abelmann and L. Abelmann and J.P.J. Groenland and \{van Honschoten\}, J.W.",

booktitle = "21st Micromechanics and Micro systems Europe Workshop, MME 2010",

address = "Netherlands",

note = "21st Micromechanics and Microsystems Europe Workshop, MME 2010 ; Conference date: 26-09-2010 Through 29-09-2010",

}

Shui, L, Jin, M, Bomer, JG, Carlen, E & van den Berg, A 2010, High-throughput on-chip DNA fragmentation. in L Abelmann, L Abelmann, JPJ Groenland & JW van Honschoten (eds), 21st Micromechanics and Micro systems Europe Workshop, MME 2010. Twente University Press (TUP), Enschede, pp. 124-127, 21st Micromechanics and Microsystems Europe Workshop, MME 2010, Enschede, Netherlands, 26/09/10.

High-throughput on-chip DNA fragmentation. / Shui, Lingling; Jin, Mingliang; Bomer, Johan G. et al.
21st Micromechanics and Micro systems Europe Workshop, MME 2010. ed. / Leon Abelmann; L. Abelmann; J.P.J. Groenland; J.W. van Honschoten. Enschede: Twente University Press (TUP), 2010. p. 124-127.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic

TY - GEN

T1 - High-throughput on-chip DNA fragmentation

AU - Shui, Lingling

AU - Jin, Mingliang

AU - Bomer, Johan G.

AU - Carlen, Edwin

AU - van den Berg, Albert

N1 - Conference code: 21

PY - 2010

Y1 - 2010

N2 - free microfluidic deoxyribonucleic acid (DNA) fragmentation chip that is based on hydrodynamic shearing. Genomic DNA has been reproducibly fragmented with 2-10 kbp fragment lengths by applying hydraulic pressure ΔP across micromachined constrictions in the microfluidic channels. The utilization of a series of constrictions reduces the variance of the fragmented DNA length distribution; and parallel microfluidic channels design eliminates the device clogging.

AB - free microfluidic deoxyribonucleic acid (DNA) fragmentation chip that is based on hydrodynamic shearing. Genomic DNA has been reproducibly fragmented with 2-10 kbp fragment lengths by applying hydraulic pressure ΔP across micromachined constrictions in the microfluidic channels. The utilization of a series of constrictions reduces the variance of the fragmented DNA length distribution; and parallel microfluidic channels design eliminates the device clogging.

KW - METIS-277553

KW - IR-76045

KW - Micro-fluidics

KW - EWI-19641

KW - Hydrodynamic shearing

KW - BIOS-Nanosensors

KW - DNA fragmentation

M3 - Conference contribution

SN - 978-90-81673716

SP - 124

EP - 127

BT - 21st Micromechanics and Micro systems Europe Workshop, MME 2010

A2 - Abelmann, Leon

A2 - Abelmann, L.

A2 - Groenland, J.P.J.

A2 - van Honschoten, J.W.

PB - Twente University Press (TUP)

CY - Enschede

T2 - 21st Micromechanics and Microsystems Europe Workshop, MME 2010

Y2 - 26 September 2010 through 29 September 2010

ER -