High-Throughput Screening Assay Identifies Small Molecules Capable of Modulating the BMP-2 and TGF-β1 Signaling Pathway

Corina-Adriana Ghebes, Jeré van Lente, Janine Nicole Post, Daniël B.F. Saris*, Hugo Fernandes

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Modulating the bone morphogenetic protein 2 (BMP-2) and transforming growth factor–β1 (TGF-β1) signaling pathways is essential during tendon/ligament (T/L) healing. Unfortunately, growth factor delivery in situ is far from trivial and, in many cases, the necessary growth factors are not approved for clinical use. Here we used a BMP-2 and a TGF-β1 reporter cell line to screen a library of 1280 Food and Drug Administration–approved small molecules and identify modulators of both signaling pathways. We identified four compounds capable of modulating BMP and TGF signaling on primary human tendon–derived cells (huTCs) and describe their effects on proliferation and differentiation of these cells.
Original languageEnglish
Pages (from-to)40-50
Number of pages11
JournalSLAS DISCOVERY: Advancing Life Sciences R&D
Volume22
Issue number1
Early online date14 Sep 2016
DOIs
Publication statusPublished - 1 Jan 2017

Keywords

  • BMP-2 signaling
  • Small-molecule discovery
  • Tendon/ligament
  • TGF-β1 signaling
  • IR-102828
  • METIS-320218

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