High-Throughput Screening Assay Identifies Small Molecules Capable of Modulating the BMP-2 and TGF-β1 Signaling Pathway

Corina-Adriana Ghebes; Jeré van Lente; Janine Nicole Post; Daniël B.F. Saris; Hugo Fernandes

doi:10.1177/1087057116669346

High-Throughput Screening Assay Identifies Small Molecules Capable of Modulating the BMP-2 and TGF-β1 Signaling Pathway

Corina-Adriana Ghebes, Jeré van Lente, Janine Nicole Post, Daniël B.F. Saris^*, Hugo Fernandes

^*Corresponding author for this work

Developmental BioEngineering

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Modulating the bone morphogenetic protein 2 (BMP-2) and transforming growth factor–β1 (TGF-β1) signaling pathways is essential during tendon/ligament (T/L) healing. Unfortunately, growth factor delivery in situ is far from trivial and, in many cases, the necessary growth factors are not approved for clinical use. Here we used a BMP-2 and a TGF-β1 reporter cell line to screen a library of 1280 Food and Drug Administration–approved small molecules and identify modulators of both signaling pathways. We identified four compounds capable of modulating BMP and TGF signaling on primary human tendon–derived cells (huTCs) and describe their effects on proliferation and differentiation of these cells.

Original language	English
Pages (from-to)	40-50
Number of pages	11
Journal	SLAS DISCOVERY: Advancing Life Sciences R&D
Volume	22
Issue number	1
Early online date	14 Sept 2016
DOIs	https://doi.org/10.1177/1087057116669346
Publication status	Published - 1 Jan 2017

Keywords

BMP-2 signaling
Small-molecule discovery
Tendon/ligament
TGF-β1 signaling

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1177/1087057116669346

ArticleFinal published version, 3.59 MBLicence: Taverne
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Cite this

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abstract = "Modulating the bone morphogenetic protein 2 (BMP-2) and transforming growth factor–β1 (TGF-β1) signaling pathways is essential during tendon/ligament (T/L) healing. Unfortunately, growth factor delivery in situ is far from trivial and, in many cases, the necessary growth factors are not approved for clinical use. Here we used a BMP-2 and a TGF-β1 reporter cell line to screen a library of 1280 Food and Drug Administration–approved small molecules and identify modulators of both signaling pathways. We identified four compounds capable of modulating BMP and TGF signaling on primary human tendon–derived cells (huTCs) and describe their effects on proliferation and differentiation of these cells.",

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AU - Saris, Daniël B.F.

AU - Fernandes, Hugo

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High-Throughput Screening Assay Identifies Small Molecules Capable of Modulating the BMP-2 and TGF-β1 Signaling Pathway

Abstract

Keywords

UN SDGs

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