High-throughput surface plasmon resonance imaging-based biomolecular kinetic screening analysis

Ganeshram Krishnamoorthy*, J. Bianca Beusink, Richard B.M. Schasfoort

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    8 Citations (Scopus)

    Abstract

    In this paper we show a high-throughput method to screen the kinetics and affinity constants of many biomolecular interactions simultaneously. During the preparation of the sensor chip, ligands were serially diluted and spotted in a 6 × 4 microarray on the sensor surface. A multi-analyte sample was injected and the real-time surface plasmon resonance (SPR) responses of all the 24 microarray spots were obtained using a commercial SPR imaging instrument. The multi responses of the association and dissociation processes obtained from a single analyte injection are sufficient to calculate the rates and affinity constants of the interactions between three interactant antigen-antibody pairs using a simple monophasic kinetic model. The method drastically reduces the measurement time and cost in the benefit of increased throughput.

    Original languageEnglish
    Pages (from-to)1020-1025
    Number of pages6
    JournalAnalytical methods
    Volume2
    Issue number8
    DOIs
    Publication statusPublished - 1 Aug 2010

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