Abstract
| Original language | Undefined |
|---|---|
| Pages (from-to) | 1076-1085 |
| Number of pages | 10 |
| Journal | Small |
| Volume | 9 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 8 Apr 2013 |
Keywords
- natural nanopores
- EWI-23776
- Confocal Microscopy
- METIS-300046
- IR-87418
- Membranes
- Micro-fluidics
- BLM
Cite this
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High yield, reproducible and quasi-automated bilayer formation in a microfluidic format. / Schulze Greiving-Stimberg, Verena Carolin; Bomer, Johan G.; van Uitert, I.; van den Berg, Albert; le Gac, Severine.
In: Small, Vol. 9, No. 7, 08.04.2013, p. 1076-1085.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - High yield, reproducible and quasi-automated bilayer formation in a microfluidic format
AU - Schulze Greiving-Stimberg, Verena Carolin
AU - Bomer, Johan G.
AU - van Uitert, I.
AU - van den Berg, Albert
AU - le Gac, Severine
N1 - eemcs-eprint-23776
PY - 2013/4/8
Y1 - 2013/4/8
N2 - A microfluidic platform is reported for various experimentation schemes on cell membrane models and membrane proteins using a combination of electrical and optical measurements, including confocal microscopy. Bilayer lipid membranes (BLMs) are prepared in the device upon spontaneous and instantaneous thinning of the lipid solution in a 100-m dry-etched aperture in a 12.5-m thick Teflon foil. Using this quasi-automated approach, a remarkable 100% membrane formation yield is reached (including reflushing in 4% of the cases), and BLMs are stable for up to 36 h. Furthermore, the potential of this platform is demonstrated for (i) the in-depth characterization of BLMs comprising both synthetic and natural lipids (1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) and L--phosphatidylcholine (L--PC)/cholesterol, respectively) in terms of seal resistance, capacitance, surface area, specific capacitance, and membrane hydrophobic thickness; (ii) confocal microscopy imaging of phase separation in sphingomyelin/L--PC/cholesterol ternary membranes; (iii) electrical measurements of individual nanopores (-hemolysin, gramicidin); and (iv) indirect assessment of the alteration of membrane properties upon exposure to chemical stimuli using the natural nanopore gramicidin as a sensor
AB - A microfluidic platform is reported for various experimentation schemes on cell membrane models and membrane proteins using a combination of electrical and optical measurements, including confocal microscopy. Bilayer lipid membranes (BLMs) are prepared in the device upon spontaneous and instantaneous thinning of the lipid solution in a 100-m dry-etched aperture in a 12.5-m thick Teflon foil. Using this quasi-automated approach, a remarkable 100% membrane formation yield is reached (including reflushing in 4% of the cases), and BLMs are stable for up to 36 h. Furthermore, the potential of this platform is demonstrated for (i) the in-depth characterization of BLMs comprising both synthetic and natural lipids (1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) and L--phosphatidylcholine (L--PC)/cholesterol, respectively) in terms of seal resistance, capacitance, surface area, specific capacitance, and membrane hydrophobic thickness; (ii) confocal microscopy imaging of phase separation in sphingomyelin/L--PC/cholesterol ternary membranes; (iii) electrical measurements of individual nanopores (-hemolysin, gramicidin); and (iv) indirect assessment of the alteration of membrane properties upon exposure to chemical stimuli using the natural nanopore gramicidin as a sensor
KW - natural nanopores
KW - EWI-23776
KW - Confocal Microscopy
KW - METIS-300046
KW - IR-87418
KW - Membranes
KW - Micro-fluidics
KW - BLM
U2 - 10.1002/smll.201201821
DO - 10.1002/smll.201201821
M3 - Article
VL - 9
SP - 1076
EP - 1085
JO - Small
JF - Small
SN - 1613-6810
IS - 7
ER -