Highly parallel transport recordings on a membrane-on-nanopore chip at single molecule resolution

M. Urban; A. Kleefen; N. Mukherjee; P. Seelheim; B. Windschiegl; M. Vor der Brüggen; A. Koçer; R. Tampé

doi:10.1021/nl5002873

Highly parallel transport recordings on a membrane-on-nanopore chip at single molecule resolution

M. Urban, A. Kleefen, N. Mukherjee, P. Seelheim, B. Windschiegl, M. Vor der Brüggen, A. Koçer, R. Tampé^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

37 Citations (Scopus)

Abstract

Membrane proteins are prime drug targets as they control the transit of information, ions, and solutes across membranes. Here, we present a membrane-on-nanopore platform to analyze nonelectrogenic channels and transporters that are typically not accessible by electrophysiological methods in a multiplexed manner. The silicon chip contains 250 000 femtoliter cavities, closed by a silicon dioxide top layer with defined nanopores. Lipid vesicles containing membrane proteins of interest are spread onto the nanopore-chip surface. Transport events of ligand-gated channels were recorded at single-molecule resolution by high-parallel fluorescence decoding.

Original language	English
Pages (from-to)	1674-1680
Journal	Nano letters
Volume	14
Issue number	3
DOIs	https://doi.org/10.1021/nl5002873
Publication status	Published - 2014
Externally published	Yes

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10.1021/nl5002873

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abstract = "Membrane proteins are prime drug targets as they control the transit of information, ions, and solutes across membranes. Here, we present a membrane-on-nanopore platform to analyze nonelectrogenic channels and transporters that are typically not accessible by electrophysiological methods in a multiplexed manner. The silicon chip contains 250 000 femtoliter cavities, closed by a silicon dioxide top layer with defined nanopores. Lipid vesicles containing membrane proteins of interest are spread onto the nanopore-chip surface. Transport events of ligand-gated channels were recorded at single-molecule resolution by high-parallel fluorescence decoding.",

author = "M. Urban and A. Kleefen and N. Mukherjee and P. Seelheim and B. Windschiegl and {Vor der Br{\"u}ggen}, M. and A. Ko{\c c}er and R. Tamp{\'e}",

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doi = "10.1021/nl5002873",

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T1 - Highly parallel transport recordings on a membrane-on-nanopore chip at single molecule resolution

AU - Urban, M.

AU - Kleefen, A.

AU - Mukherjee, N.

AU - Seelheim, P.

AU - Windschiegl, B.

AU - Vor der Brüggen, M.

AU - Koçer, A.

AU - Tampé, R.

PY - 2014

Y1 - 2014

N2 - Membrane proteins are prime drug targets as they control the transit of information, ions, and solutes across membranes. Here, we present a membrane-on-nanopore platform to analyze nonelectrogenic channels and transporters that are typically not accessible by electrophysiological methods in a multiplexed manner. The silicon chip contains 250 000 femtoliter cavities, closed by a silicon dioxide top layer with defined nanopores. Lipid vesicles containing membrane proteins of interest are spread onto the nanopore-chip surface. Transport events of ligand-gated channels were recorded at single-molecule resolution by high-parallel fluorescence decoding.

AB - Membrane proteins are prime drug targets as they control the transit of information, ions, and solutes across membranes. Here, we present a membrane-on-nanopore platform to analyze nonelectrogenic channels and transporters that are typically not accessible by electrophysiological methods in a multiplexed manner. The silicon chip contains 250 000 femtoliter cavities, closed by a silicon dioxide top layer with defined nanopores. Lipid vesicles containing membrane proteins of interest are spread onto the nanopore-chip surface. Transport events of ligand-gated channels were recorded at single-molecule resolution by high-parallel fluorescence decoding.

UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-84896335391&partnerID=MN8TOARS

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M3 - Article

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VL - 14

SP - 1674

EP - 1680

JO - Nano letters

JF - Nano letters

IS - 3

ER -

Highly parallel transport recordings on a membrane-on-nanopore chip at single molecule resolution

Abstract

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