Highly parallelized human embryonic stem cell differentiation in nanoliter chambers

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Abstract

We differentiate human embryonic stem cells (hESC) to cardiac mesodermal cells in low volume chambers (30 nL) using different time intervals for the discontinuous exchange of cell culture medium. Our chip has 64 independently addressable chambers in which the medium is exchanged automatically every 1 - 8 hours. We show that the hESC proliferation rate decreases at long medium exchange intervals (8 hours). Furthermore, we show that shorter medium exchange intervals (hourly and bihourly) during differentiation lead to a higher fluorescence intensity of the early cardiac mesoderm reporter MESP1mCherry on day 3 of differentiation.

Original languageEnglish
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherThe Chemical and Biological Microsystems Society
Pages861-862
Number of pages2
ISBN (Electronic)9781733419017
Publication statusPublished - 2020
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2020 - Virtual Event
Duration: 4 Oct 20209 Oct 2020
Conference number: 24
https://microtas2020.org/

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2020
Abbreviated titleMicroTAS 2020
CityVirtual Event
Period4/10/209/10/20
Internet address

Keywords

  • Discontinuous medium perfusion
  • Parallelization
  • Stem cell differentiation

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