Abstract
We differentiate human embryonic stem cells (hESC) to cardiac mesodermal cells in low volume chambers (30 nL) using different time intervals for the discontinuous exchange of cell culture medium. Our chip has 64 independently addressable chambers in which the medium is exchanged automatically every 1 - 8 hours. We show that the hESC proliferation rate decreases at long medium exchange intervals (8 hours). Furthermore, we show that shorter medium exchange intervals (hourly and bihourly) during differentiation lead to a higher fluorescence intensity of the early cardiac mesoderm reporter MESP1mCherry on day 3 of differentiation.
Original language | English |
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Title of host publication | MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
Publisher | The Chemical and Biological Microsystems Society |
Pages | 861-862 |
Number of pages | 2 |
ISBN (Electronic) | 9781733419017 |
Publication status | Published - 2020 |
Event | 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2020 - Virtual Event Duration: 4 Oct 2020 → 9 Oct 2020 Conference number: 24 https://microtas2020.org/ |
Conference
Conference | 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2020 |
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Abbreviated title | MicroTAS 2020 |
City | Virtual Event |
Period | 4/10/20 → 9/10/20 |
Internet address |
Keywords
- Discontinuous medium perfusion
- Parallelization
- Stem cell differentiation