Highly Sensitive Protein Detection by Asymmetric Mach-Zehnder Interferometry for Biosensing Applications

Melissa J. Goodwin, Geert A.J. Besselink, Floris Falke, Arnoud S. Everhardt, Jeroen J.L.M. Cornelissen, Jurriaan Huskens*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)
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The sensitivity and performance of an asymmetric Mach-Zehnder interferometer (aMZI) were compared to those of quartz crystal microbalance with dissipation (QCM-D). The binding of streptavidin to sensor chips coated with poly-l-lysine (PLL), modified with biotin and oligoethyleneglycol (OEG) (PLL-biotin), was used to compare the binding signals obtained from both technologies. PLL-biotin proved to be an efficient method to add bioreceptors to both the QCM-D and aMZI chips. The final, saturated value of streptavidin binding was compared with those from aMZI (253 ng cm-2) and QCM-D (460 ng cm-2). These values were then used to evaluate that 45% of the measured streptavidin mass in the QCM-D came from hydrodynamically coupled water. Importantly, the signal-to-noise ratio of the aMZI was found to be 200 times higher than that of the QCM-D. These results indicate the potential of the aMZI platform for highly sensitive and accurate biosensing applications.

Original languageEnglish
Pages (from-to)4566-4572
Number of pages7
JournalACS Applied Bio Materials
Issue number7
Publication statusPublished - 20 Jul 2020


  • UT-Hybrid-D
  • Biosensing
  • Poly-l-lysine
  • Quartz crystal microbalance (QCM)
  • Surface plasmon resonance
  • Asymmetric Mach-Zehnder interferometry


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