TY - JOUR
T1 - Highly Sensitive Protein Detection by Asymmetric Mach-Zehnder Interferometry for Biosensing Applications
AU - Goodwin, Melissa J.
AU - Besselink, Geert A.J.
AU - Falke, Floris
AU - Everhardt, Arnoud S.
AU - Cornelissen, Jeroen J.L.M.
AU - Huskens, Jurriaan
N1 - ACS deal
PY - 2020/7/20
Y1 - 2020/7/20
N2 - The sensitivity and performance of an asymmetric Mach-Zehnder interferometer (aMZI) were compared to those of quartz crystal microbalance with dissipation (QCM-D). The binding of streptavidin to sensor chips coated with poly-l-lysine (PLL), modified with biotin and oligoethyleneglycol (OEG) (PLL-biotin), was used to compare the binding signals obtained from both technologies. PLL-biotin proved to be an efficient method to add bioreceptors to both the QCM-D and aMZI chips. The final, saturated value of streptavidin binding was compared with those from aMZI (253 ng cm-2) and QCM-D (460 ng cm-2). These values were then used to evaluate that 45% of the measured streptavidin mass in the QCM-D came from hydrodynamically coupled water. Importantly, the signal-to-noise ratio of the aMZI was found to be 200 times higher than that of the QCM-D. These results indicate the potential of the aMZI platform for highly sensitive and accurate biosensing applications.
AB - The sensitivity and performance of an asymmetric Mach-Zehnder interferometer (aMZI) were compared to those of quartz crystal microbalance with dissipation (QCM-D). The binding of streptavidin to sensor chips coated with poly-l-lysine (PLL), modified with biotin and oligoethyleneglycol (OEG) (PLL-biotin), was used to compare the binding signals obtained from both technologies. PLL-biotin proved to be an efficient method to add bioreceptors to both the QCM-D and aMZI chips. The final, saturated value of streptavidin binding was compared with those from aMZI (253 ng cm-2) and QCM-D (460 ng cm-2). These values were then used to evaluate that 45% of the measured streptavidin mass in the QCM-D came from hydrodynamically coupled water. Importantly, the signal-to-noise ratio of the aMZI was found to be 200 times higher than that of the QCM-D. These results indicate the potential of the aMZI platform for highly sensitive and accurate biosensing applications.
KW - UT-Hybrid-D
KW - Biosensing
KW - Poly-l-lysine
KW - Quartz crystal microbalance (QCM)
KW - Surface plasmon resonance
KW - Asymmetric Mach-Zehnder interferometry
UR - http://www.scopus.com/inward/record.url?scp=85090223322&partnerID=8YFLogxK
U2 - 10.1021/acsabm.0c00491
DO - 10.1021/acsabm.0c00491
M3 - Article
AN - SCOPUS:85090223322
SN - 2576-6422
VL - 3
SP - 4566
EP - 4572
JO - ACS Applied Bio Materials
JF - ACS Applied Bio Materials
IS - 7
ER -