Holographic characterization of contaminants in water: Differentiation of suspended particles in heterogeneous dispersions

Laura A. Philips*, David B. Ruffner, Fook Chiong Cheong, Jaroslaw M. Blusewicz, Priya Kasimbeg, Basma Waisi, Jeffrey R. McCutcheon, David G. Grier

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)
80 Downloads (Pure)

Abstract

Determining the size distribution and composition of particles suspended in water can be challenging in heterogeneous multicomponent samples. Light scattering techniques can measure the distribution of particle sizes, but provide no basis for distinguishing different types of particles. Direct imaging techniques can categorize particles by shape, but offer few insights into their composition. Holographic characterization meets this need by directly measuring the size, refractive index, and three-dimensional position of individual particles in a suspension. The ability to measure an individual colloidal particle's refractive index is a unique capability of holographic characterization. Holographic characterization is fast enough, moreover, to build up population distribution data in real time, and to track time variations in the concentrations of different dispersed populations of particles. We demonstrate these capabilities using a model system consisting of polystyrene microbeads co-dispersed with bacteria in an oil-in-water emulsion. We also demonstrate how the holographic fingerprint of different contaminants can contribute to identifying their source.

Original languageEnglish
Pages (from-to)431-439
Number of pages9
JournalWater research
Volume122
DOIs
Publication statusPublished - Oct 2017

Keywords

  • Bacteria
  • Colloid
  • Concentration measurement
  • Emulsion
  • Holographic microscopy
  • Particle characterization

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