Host–guest extraction of immunoglobulin G using calix[6]arenas

M. Martínez-Aragón, E.L.V. Goetheer, A.B. de Haan

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Host–guest interactions have been widely employed in various techniques in biochemistry in the last decades. In this way, the use of calix[6]arenes modified with a carboxylic acid as host in the host–guest extraction of immunoglobulin G (IgG) is studied. It is possible to extract IgG from an aqueous medium. Different operational parameters such as pH, salt concentration and stirring speed were evaluated. At high pH, up to 50% of the IgG can be transferred to the organic phase. The study of pH demonstrates that the interaction mechanism depends on a proton exchange mechanism together with hydrogen-bonding. Salt concentration has a direct effect on the interaction strength of the lysine residues of IgG and the calix[6]arene, leading to a diminished extraction at high salt concentrations. For the prevention of denaturation the contact mode and intensity of mixing are crucial. Based on the data obtained fromkinetic and equilibrium studies a suggestion for the extraction mechanism is made.
Original languageUndefined
Pages (from-to)73-78
JournalSeparation and purification technology
Issue number1
Publication statusPublished - 2009


  • Immunoglobulin
  • Calix[6]arene
  • IR-80095
  • Extraction

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