Host–guest extraction of immunoglobulin G using calix[6]arenas

M. Martínez-Aragón, E.L.V. Goetheer, A.B. de Haan

Research output: Contribution to journalArticleAcademic

16 Citations (Scopus)

Abstract

Host–guest interactions have been widely employed in various techniques in biochemistry in the last decades. In this way, the use of calix[6]arenes modified with a carboxylic acid as host in the host–guest extraction of immunoglobulin G (IgG) is studied. It is possible to extract IgG from an aqueous medium. Different operational parameters such as pH, salt concentration and stirring speed were evaluated. At high pH, up to 50% of the IgG can be transferred to the organic phase. The study of pH demonstrates that the interaction mechanism depends on a proton exchange mechanism together with hydrogen-bonding. Salt concentration has a direct effect on the interaction strength of the lysine residues of IgG and the calix[6]arene, leading to a diminished extraction at high salt concentrations. For the prevention of denaturation the contact mode and intensity of mixing are crucial. Based on the data obtained fromkinetic and equilibrium studies a suggestion for the extraction mechanism is made.
Original languageUndefined
Pages (from-to)73-78
JournalSeparation and purification technology
Volume65
Issue number1
DOIs
Publication statusPublished - 2009

Keywords

  • Immunoglobulin
  • Calix[6]arene
  • IR-80095
  • Extraction

Cite this

Martínez-Aragón, M. ; Goetheer, E.L.V. ; de Haan, A.B. / Host–guest extraction of immunoglobulin G using calix[6]arenas. In: Separation and purification technology. 2009 ; Vol. 65, No. 1. pp. 73-78.
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abstract = "Host–guest interactions have been widely employed in various techniques in biochemistry in the last decades. In this way, the use of calix[6]arenes modified with a carboxylic acid as host in the host–guest extraction of immunoglobulin G (IgG) is studied. It is possible to extract IgG from an aqueous medium. Different operational parameters such as pH, salt concentration and stirring speed were evaluated. At high pH, up to 50{\%} of the IgG can be transferred to the organic phase. The study of pH demonstrates that the interaction mechanism depends on a proton exchange mechanism together with hydrogen-bonding. Salt concentration has a direct effect on the interaction strength of the lysine residues of IgG and the calix[6]arene, leading to a diminished extraction at high salt concentrations. For the prevention of denaturation the contact mode and intensity of mixing are crucial. Based on the data obtained fromkinetic and equilibrium studies a suggestion for the extraction mechanism is made.",
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Host–guest extraction of immunoglobulin G using calix[6]arenas. / Martínez-Aragón, M.; Goetheer, E.L.V.; de Haan, A.B.

In: Separation and purification technology, Vol. 65, No. 1, 2009, p. 73-78.

Research output: Contribution to journalArticleAcademic

TY - JOUR

T1 - Host–guest extraction of immunoglobulin G using calix[6]arenas

AU - Martínez-Aragón, M.

AU - Goetheer, E.L.V.

AU - de Haan, A.B.

PY - 2009

Y1 - 2009

N2 - Host–guest interactions have been widely employed in various techniques in biochemistry in the last decades. In this way, the use of calix[6]arenes modified with a carboxylic acid as host in the host–guest extraction of immunoglobulin G (IgG) is studied. It is possible to extract IgG from an aqueous medium. Different operational parameters such as pH, salt concentration and stirring speed were evaluated. At high pH, up to 50% of the IgG can be transferred to the organic phase. The study of pH demonstrates that the interaction mechanism depends on a proton exchange mechanism together with hydrogen-bonding. Salt concentration has a direct effect on the interaction strength of the lysine residues of IgG and the calix[6]arene, leading to a diminished extraction at high salt concentrations. For the prevention of denaturation the contact mode and intensity of mixing are crucial. Based on the data obtained fromkinetic and equilibrium studies a suggestion for the extraction mechanism is made.

AB - Host–guest interactions have been widely employed in various techniques in biochemistry in the last decades. In this way, the use of calix[6]arenes modified with a carboxylic acid as host in the host–guest extraction of immunoglobulin G (IgG) is studied. It is possible to extract IgG from an aqueous medium. Different operational parameters such as pH, salt concentration and stirring speed were evaluated. At high pH, up to 50% of the IgG can be transferred to the organic phase. The study of pH demonstrates that the interaction mechanism depends on a proton exchange mechanism together with hydrogen-bonding. Salt concentration has a direct effect on the interaction strength of the lysine residues of IgG and the calix[6]arene, leading to a diminished extraction at high salt concentrations. For the prevention of denaturation the contact mode and intensity of mixing are crucial. Based on the data obtained fromkinetic and equilibrium studies a suggestion for the extraction mechanism is made.

KW - Immunoglobulin

KW - Calix[6]arene

KW - IR-80095

KW - Extraction

U2 - 10.1016/j.seppur.2008.03.017

DO - 10.1016/j.seppur.2008.03.017

M3 - Article

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SP - 73

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JO - Separation and purification technology

JF - Separation and purification technology

SN - 1383-5866

IS - 1

ER -