Abstract
Model systems with defined genetic modifications are powerful tools for basic research and translational disease modelling. Fortunately, generating state-of-the-art genetic model systems is becoming more accessible to non-geneticists due to advances in genome editing technologies. As a consequence, solely relying on (transient) overexpression of (mutant) effector proteins is no longer recommended since scientific standards increasingly demand genetic modification of endogenous loci. In this review, we provide up-to-date guidelines with respect to homology-directed repair (HDR)-mediated editing of mammalian model systems, aimed at assisting researchers in designing an efficient genome editing strategy.
Original language | English |
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Pages (from-to) | 6435-6454 |
Number of pages | 20 |
Journal | Nucleic acids research |
Volume | 46 |
Issue number | 13 |
Early online date | 28 Jun 2018 |
DOIs | |
Publication status | Published - 28 Jul 2018 |
Keywords
- Genome editing
- CRISPR
- Gene tagging