How to create state-of-the-art genetic model systems: strategies for optimal CRISPR-mediated genome editing

Yannik Bollen, Jasmin Post, Bon-Kyoung Koo, Hugo J.G. Snippert*

*Corresponding author for this work

Research output: Contribution to journalReview articleAcademicpeer-review

10 Citations (Scopus)
30 Downloads (Pure)

Abstract

Model systems with defined genetic modifications are powerful tools for basic research and translational disease modelling. Fortunately, generating state-of-the-art genetic model systems is becoming more accessible to non-geneticists due to advances in genome editing technologies. As a consequence, solely relying on (transient) overexpression of (mutant) effector proteins is no longer recommended since scientific standards increasingly demand genetic modification of endogenous loci. In this review, we provide up-to-date guidelines with respect to homology-directed repair (HDR)-mediated editing of mammalian model systems, aimed at assisting researchers in designing an efficient genome editing strategy.
Original languageEnglish
Pages (from-to)6435-6454
Number of pages20
JournalNucleic acids research
Volume46
Issue number13
Early online date28 Jun 2018
DOIs
Publication statusPublished - 28 Jul 2018

Keywords

  • Genome editing
  • CRISPR
  • Gene tagging

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