How to create state-of-the-art genetic model systems: strategies for optimal CRISPR-mediated genome editing

Yannik  Bollen; Jasmin Post; Bon-Kyoung Koo; Hugo J.G. Snippert

doi:10.1093/nar/gky571

How to create state-of-the-art genetic model systems: strategies for optimal CRISPR-mediated genome editing

Yannik Bollen, Jasmin Post, Bon-Kyoung Koo, Hugo J.G. Snippert^*

^*Corresponding author for this work

Medical Cell Biophysics

Research output: Contribution to journal › Review article › Academic › peer-review

42 Citations (Scopus)

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Abstract

Model systems with defined genetic modifications are powerful tools for basic research and translational disease modelling. Fortunately, generating state-of-the-art genetic model systems is becoming more accessible to non-geneticists due to advances in genome editing technologies. As a consequence, solely relying on (transient) overexpression of (mutant) effector proteins is no longer recommended since scientific standards increasingly demand genetic modification of endogenous loci. In this review, we provide up-to-date guidelines with respect to homology-directed repair (HDR)-mediated editing of mammalian model systems, aimed at assisting researchers in designing an efficient genome editing strategy.

Original language	English
Pages (from-to)	6435-6454
Number of pages	20
Journal	Nucleic acids research
Volume	46
Issue number	13
Early online date	28 Jun 2018
DOIs	https://doi.org/10.1093/nar/gky571
Publication status	Published - 28 Jul 2018

Keywords

Genome editing
CRISPR
Gene tagging

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6061873/pdf/gky571.pdf

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title = "How to create state-of-the-art genetic model systems: strategies for optimal CRISPR-mediated genome editing",

abstract = "Model systems with defined genetic modifications are powerful tools for basic research and translational disease modelling. Fortunately, generating state-of-the-art genetic model systems is becoming more accessible to non-geneticists due to advances in genome editing technologies. As a consequence, solely relying on (transient) overexpression of (mutant) effector proteins is no longer recommended since scientific standards increasingly demand genetic modification of endogenous loci. In this review, we provide up-to-date guidelines with respect to homology-directed repair (HDR)-mediated editing of mammalian model systems, aimed at assisting researchers in designing an efficient genome editing strategy.",

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AU - Bollen, Yannik

AU - Post, Jasmin

AU - Koo, Bon-Kyoung

AU - Snippert, Hugo J.G.

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AB - Model systems with defined genetic modifications are powerful tools for basic research and translational disease modelling. Fortunately, generating state-of-the-art genetic model systems is becoming more accessible to non-geneticists due to advances in genome editing technologies. As a consequence, solely relying on (transient) overexpression of (mutant) effector proteins is no longer recommended since scientific standards increasingly demand genetic modification of endogenous loci. In this review, we provide up-to-date guidelines with respect to homology-directed repair (HDR)-mediated editing of mammalian model systems, aimed at assisting researchers in designing an efficient genome editing strategy.

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KW - Gene tagging

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ER -

How to create state-of-the-art genetic model systems: strategies for optimal CRISPR-mediated genome editing

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Keywords

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