Human bone marrow-derived mesenchymal cell reactions to 316L stainless steel: An in vitro study on cell viability and interleukin-6 expression

I.B. Anwar*, A. Santoso, E. Saputra, R. Ismail, J. Jamari, E. van der Heide

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    2 Citations (Scopus)
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    Abstract

    Purpose: Human bone marrow-derived mesenchymal cell (hBMC) reactions to 316L stainless steel (316L-SS) have never been evaluated. The objective of this study was to assess cell viability and interleukin-6 expression of hBMC cultures upon treatment with a 316L-SS implant. Methods: A cytotoxicity analysis was conducted with a 3-(4,5-dimethylthiazol 2-yl)-2,5- diphenyltetrazolium (MTT) assay after a period of 24, 48 and 72 hours of incubation. Expression of interleukin-6 was measured using enzyme-linked immunosorbent assay (ELISA).

    Results: Cell viability measurement was performed via IC50 formula. All treatment group showed a > 50 % cell viability with a range of 56,5 - 96,9 % at 24 hours, 51,8-77,3% at 48 hours and 70,1- 120 % at 72 hours. Interleukin-6 expression was downregulated subsequent to treatment with 316L-SS compared to the control group. Conclusion: We found that 316L-SS did not exhibit toxicity towards hBMC culture.

    Original languageEnglish
    Pages (from-to)335-338
    Number of pages4
    JournalAdvanced Pharmaceutical Bulletin
    Volume7
    Issue number2
    DOIs
    Publication statusPublished - 2017

    Keywords

    • 316L stainless steel
    • Cell viability
    • Human bone marrow-derived mesenchymal cells
    • Interleukin-6

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