TY - JOUR
T1 - Human bone marrow-derived mesenchymal cell reactions to 316L stainless steel
T2 - An in vitro study on cell viability and interleukin-6 expression
AU - Anwar, I.B.
AU - Santoso, A.
AU - Saputra, E.
AU - Ismail, R.
AU - Jamari, J.
AU - van der Heide, E.
PY - 2017
Y1 - 2017
N2 - Purpose: Human bone marrow-derived mesenchymal cell (hBMC) reactions to 316L stainless steel (316L-SS) have never been evaluated. The objective of this study was to assess cell viability and interleukin-6 expression of hBMC cultures upon treatment with a 316L-SS implant. Methods: A cytotoxicity analysis was conducted with a 3-(4,5-dimethylthiazol 2-yl)-2,5- diphenyltetrazolium (MTT) assay after a period of 24, 48 and 72 hours of incubation. Expression of interleukin-6 was measured using enzyme-linked immunosorbent assay (ELISA).Results: Cell viability measurement was performed via IC50 formula. All treatment group showed a > 50 % cell viability with a range of 56,5 - 96,9 % at 24 hours, 51,8-77,3% at 48 hours and 70,1- 120 % at 72 hours. Interleukin-6 expression was downregulated subsequent to treatment with 316L-SS compared to the control group. Conclusion: We found that 316L-SS did not exhibit toxicity towards hBMC culture.
AB - Purpose: Human bone marrow-derived mesenchymal cell (hBMC) reactions to 316L stainless steel (316L-SS) have never been evaluated. The objective of this study was to assess cell viability and interleukin-6 expression of hBMC cultures upon treatment with a 316L-SS implant. Methods: A cytotoxicity analysis was conducted with a 3-(4,5-dimethylthiazol 2-yl)-2,5- diphenyltetrazolium (MTT) assay after a period of 24, 48 and 72 hours of incubation. Expression of interleukin-6 was measured using enzyme-linked immunosorbent assay (ELISA).Results: Cell viability measurement was performed via IC50 formula. All treatment group showed a > 50 % cell viability with a range of 56,5 - 96,9 % at 24 hours, 51,8-77,3% at 48 hours and 70,1- 120 % at 72 hours. Interleukin-6 expression was downregulated subsequent to treatment with 316L-SS compared to the control group. Conclusion: We found that 316L-SS did not exhibit toxicity towards hBMC culture.
KW - 316L stainless steel
KW - Cell viability
KW - Human bone marrow-derived mesenchymal cells
KW - Interleukin-6
UR - http://www.scopus.com/inward/record.url?scp=85021405698&partnerID=8YFLogxK
U2 - 10.15171/apb.2017.040
DO - 10.15171/apb.2017.040
M3 - Article
AN - SCOPUS:85021405698
SN - 2228-5881
VL - 7
SP - 335
EP - 338
JO - Advanced Pharmaceutical Bulletin
JF - Advanced Pharmaceutical Bulletin
IS - 2
ER -