Human derived cortical excitatory neurospheroids showed spontaneous activity on micro electrodes array

L. Muzzi, M. Falappa, A. Maccione, D. Di Lisa, M. Frega, S. Martinoia

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Human-induced pluripotent stem cells (hiPSCs) with their differentiation protocols, constitute a potential tool to investigate the various biological mechanisms of different human cells, such as those of the central nervous system. With the advent of such technique, we have increased the knowledge of biological mechanisms of neural diseases and, new therapies are now emerging. In particular, three-dimensional (3D) neural cell culture models including brain organoids and neurospheroids are increasingly used as in vitro platforms for studying human brain cell biology and drug screening, in genome engineering and transplantation as potential treatment for some neurodegenerative diseases. In this work, we exploited a particular differentiation protocol to generate engineered excitatory cortical neurospheroids of human origin. To assess functional network activity, we used standard Micro Electrodes Arrays (60 channels) and for the first time CMOS based devices (4096 channels). Sample cultures showed electrophysiological activity in 4 weeks and these first results suggest future possible applications for drug screening and transplantation.

Original languageEnglish
Title of host publication2021 10th International IEEE/EMBS Conference on Neural Engineering, NER 2021
PublisherIEEE Computer Society Press
Pages123-127
Number of pages5
ISBN (Electronic)9781728143378
DOIs
Publication statusPublished - 4 May 2021
Event10th International IEEE/EMBS Conference on Neural Engineering, NER 2021 - Virtual, Online, Italy
Duration: 4 May 20216 May 2021
Conference number: 10

Publication series

NameInternational IEEE/EMBS Conference on Neural Engineering, NER
Volume2021-May
ISSN (Print)1948-3546
ISSN (Electronic)1948-3554

Conference

Conference10th International IEEE/EMBS Conference on Neural Engineering, NER 2021
Abbreviated titleNER 2021
CountryItaly
CityVirtual, Online
Period4/05/216/05/21

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