Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices

Hugo J. Albers, João P. da Silva Simão, Heleen H.T. Middelkamp, Christine L. Mummery, Robert Passier, Albert van den Berg, Valeria V. Orlova, Andries D. van der Meer

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Abstract

A microfluidic thrombosis-on-a-chip platform was developed to compare the pro-thrombotic response of healthy and inflamed monolayers of human umbilical vein endothelial cells (HUVECs) and human induced pluripotent stem cell-derived endothelial cells (hiPSC-ECs). Inflammation was induced by exposing the endothelial cells (ECs) to an inflammatory cytokine Tumor Necrosis Factor-α (TNF-α). After human whole blood perfusion at an arterial shear rate, the platelet coverage and average clot size were determined. Healthy endothelium showed a lower platelet coverage than inflamed endothelium. A minor difference was measured for both platelet coverage and average clot sizes on inflamed HUVECs versus hiPSC-ECs.
Original languageEnglish
Title of host publicationMicroTas 2018
Subtitle of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences
EditorsFan-Gang Tseng, Gwo-Bin Lee
PublisherThe Chemical and Biological Microsystems Society
Pages2392-2393
ISBN (Electronic)978-1-510-89757-1
ISBN (Print)978-0-578-40530-8
Publication statusPublished - 11 Nov 2018
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018 - Kaohsiung Exhibition Center, Kaohsiung, Taiwan, Province of China
Duration: 10 Nov 201815 Nov 2018
Conference number: 22
https://cbmsociety.org/conferences/microtas2018/

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018
Abbreviated titleMicroTAS 2018
CountryTaiwan, Province of China
CityKaohsiung
Period10/11/1815/11/18
Internet address

Keywords

  • Thrombosis
  • Organ-on-a-chip
  • Vasculature
  • Human induced pluripotent stem cells
  • Endothelial cells
  • Human umbilical vein endothelial cells
  • Whole blood
  • Microfluidics

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  • Cite this

    Albers, H. J., da Silva Simão, J. P., Middelkamp, H. H. T., Mummery, C. L., Passier, R., van den Berg, A., ... van der Meer, A. D. (2018). Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices. In F-G. Tseng, & G-B. Lee (Eds.), MicroTas 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 2392-2393). The Chemical and Biological Microsystems Society.