Abstract
A microfluidic thrombosis-on-a-chip platform was developed to compare the pro-thrombotic response of healthy and inflamed monolayers of human umbilical vein endothelial cells (HUVECs) and human induced pluripotent stem cell-derived endothelial cells (hiPSC-ECs). Inflammation was induced by exposing the endothelial cells (ECs) to an inflammatory cytokine Tumor Necrosis Factor-α (TNF-α). After human whole blood perfusion at an arterial shear rate, the platelet coverage and average clot size were determined. Healthy endothelium showed a lower platelet coverage than inflamed endothelium. A minor difference was measured for both platelet coverage and average clot sizes on inflamed HUVECs versus hiPSC-ECs.
Original language | English |
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Title of host publication | MicroTas 2018 |
Subtitle of host publication | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences |
Editors | Fan-Gang Tseng, Gwo-Bin Lee |
Publisher | The Chemical and Biological Microsystems Society |
Pages | 2392-2393 |
ISBN (Electronic) | 978-1-510-89757-1 |
ISBN (Print) | 978-0-578-40530-8 |
Publication status | Published - 11 Nov 2018 |
Event | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018 - Kaohsiung Exhibition Center, Kaohsiung, Taiwan Duration: 10 Nov 2018 → 15 Nov 2018 Conference number: 22 https://cbmsociety.org/conferences/microtas2018/ |
Conference
Conference | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018 |
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Abbreviated title | MicroTAS 2018 |
Country/Territory | Taiwan |
City | Kaohsiung |
Period | 10/11/18 → 15/11/18 |
Internet address |
Keywords
- Thrombosis
- Organ-on-a-chip
- Vasculature
- Human induced pluripotent stem cells
- Endothelial cells
- Human umbilical vein endothelial cells
- Whole blood
- Microfluidics