Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices

Hugo J. Albers; João P. da Silva Simão; Heleen H.T. Middelkamp; Christine L. Mummery; Robert Passier; Albert  van den Berg; Valeria V. Orlova; Andries D. van der Meer

Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices

Hugo J. Albers, João P. da Silva Simão, Heleen H.T. Middelkamp, Christine L. Mummery, Robert Passier, Albert van den Berg, Valeria V. Orlova, Andries D. van der Meer

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

A microfluidic thrombosis-on-a-chip platform was developed to compare the pro-thrombotic response of healthy and inflamed monolayers of human umbilical vein endothelial cells (HUVECs) and human induced pluripotent stem cell-derived endothelial cells (hiPSC-ECs). Inflammation was induced by exposing the endothelial cells (ECs) to an inflammatory cytokine Tumor Necrosis Factor-α (TNF-α). After human whole blood perfusion at an arterial shear rate, the platelet coverage and average clot size were determined. Healthy endothelium showed a lower platelet coverage than inflamed endothelium. A minor difference was measured for both platelet coverage and average clot sizes on inflamed HUVECs versus hiPSC-ECs.

Original language	English
Title of host publication	MicroTas 2018
Subtitle of host publication	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences
Editors	Fan-Gang Tseng, Gwo-Bin Lee
Publisher	The Chemical and Biological Microsystems Society
Pages	2392-2393
ISBN (Electronic)	978-1-510-89757-1
ISBN (Print)	978-0-578-40530-8
Publication status	Published - 11 Nov 2018
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018 - Kaohsiung Exhibition Center, Kaohsiung, Taiwan Duration: 10 Nov 2018 → 15 Nov 2018 Conference number: 22 https://cbmsociety.org/conferences/microtas2018/

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018
Abbreviated title	MicroTAS 2018
Country/Territory	Taiwan
City	Kaohsiung
Period	10/11/18 → 15/11/18
Internet address	https://cbmsociety.org/conferences/microtas2018/

Keywords

Thrombosis
Organ-on-a-chip
Vasculature
Human induced pluripotent stem cells
Endothelial cells
Human umbilical vein endothelial cells
Whole blood
Microfluidics

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Albers, H. J., da Silva Simão, J. P., Middelkamp, H. H. T., Mummery, C. L., Passier, R., van den Berg, A., Orlova, V. V., & van der Meer, A. D. (2018). Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices. In F.-G. Tseng, & G.-B. Lee (Eds.), MicroTas 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 2392-2393). The Chemical and Biological Microsystems Society.

Albers, Hugo J. ; da Silva Simão, João P. ; Middelkamp, Heleen H.T. et al. / Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices. MicroTas 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. editor / Fan-Gang Tseng ; Gwo-Bin Lee. The Chemical and Biological Microsystems Society, 2018. pp. 2392-2393

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title = "Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices",

abstract = "A microfluidic thrombosis-on-a-chip platform was developed to compare the pro-thrombotic response of healthy and inflamed monolayers of human umbilical vein endothelial cells (HUVECs) and human induced pluripotent stem cell-derived endothelial cells (hiPSC-ECs). Inflammation was induced by exposing the endothelial cells (ECs) to an inflammatory cytokine Tumor Necrosis Factor-α (TNF-α). After human whole blood perfusion at an arterial shear rate, the platelet coverage and average clot size were determined. Healthy endothelium showed a lower platelet coverage than inflamed endothelium. A minor difference was measured for both platelet coverage and average clot sizes on inflamed HUVECs versus hiPSC-ECs.",

keywords = "Thrombosis, Organ-on-a-chip, Vasculature, Human induced pluripotent stem cells, Endothelial cells, Human umbilical vein endothelial cells, Whole blood, Microfluidics",

author = "Albers, {Hugo J.} and {da Silva Sim{\~a}o}, {Jo{\~a}o P.} and Middelkamp, {Heleen H.T.} and Mummery, {Christine L.} and Robert Passier and {van den Berg}, Albert and Orlova, {Valeria V.} and {van der Meer}, {Andries D.}",

year = "2018",

month = nov,

day = "11",

language = "English",

isbn = "978-0-578-40530-8",

pages = "2392--2393",

editor = "Fan-Gang Tseng and Gwo-Bin Lee",

booktitle = "MicroTas 2018",

publisher = "The Chemical and Biological Microsystems Society",

note = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018, MicroTAS 2018 ; Conference date: 10-11-2018 Through 15-11-2018",

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Albers, HJ, da Silva Simão, JP, Middelkamp, HHT, Mummery, CL, Passier, R , van den Berg, A, Orlova, VV & van der Meer, AD 2018, Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices. in F-G Tseng & G-B Lee (eds), MicroTas 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. The Chemical and Biological Microsystems Society, pp. 2392-2393, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018, Kaohsiung, Taiwan, 10/11/18.

Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices. / Albers, Hugo J.; da Silva Simão, João P.; Middelkamp, Heleen H.T. et al.
MicroTas 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. ed. / Fan-Gang Tseng; Gwo-Bin Lee. The Chemical and Biological Microsystems Society, 2018. p. 2392-2393.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices

AU - Albers, Hugo J.

AU - da Silva Simão, João P.

AU - Middelkamp, Heleen H.T.

AU - Mummery, Christine L.

AU - Passier, Robert

AU - van den Berg, Albert

AU - Orlova, Valeria V.

AU - van der Meer, Andries D.

N1 - Conference code: 22

PY - 2018/11/11

Y1 - 2018/11/11

N2 - A microfluidic thrombosis-on-a-chip platform was developed to compare the pro-thrombotic response of healthy and inflamed monolayers of human umbilical vein endothelial cells (HUVECs) and human induced pluripotent stem cell-derived endothelial cells (hiPSC-ECs). Inflammation was induced by exposing the endothelial cells (ECs) to an inflammatory cytokine Tumor Necrosis Factor-α (TNF-α). After human whole blood perfusion at an arterial shear rate, the platelet coverage and average clot size were determined. Healthy endothelium showed a lower platelet coverage than inflamed endothelium. A minor difference was measured for both platelet coverage and average clot sizes on inflamed HUVECs versus hiPSC-ECs.

AB - A microfluidic thrombosis-on-a-chip platform was developed to compare the pro-thrombotic response of healthy and inflamed monolayers of human umbilical vein endothelial cells (HUVECs) and human induced pluripotent stem cell-derived endothelial cells (hiPSC-ECs). Inflammation was induced by exposing the endothelial cells (ECs) to an inflammatory cytokine Tumor Necrosis Factor-α (TNF-α). After human whole blood perfusion at an arterial shear rate, the platelet coverage and average clot size were determined. Healthy endothelium showed a lower platelet coverage than inflamed endothelium. A minor difference was measured for both platelet coverage and average clot sizes on inflamed HUVECs versus hiPSC-ECs.

KW - Thrombosis

KW - Organ-on-a-chip

KW - Vasculature

KW - Human induced pluripotent stem cells

KW - Endothelial cells

KW - Human umbilical vein endothelial cells

KW - Whole blood

KW - Microfluidics

M3 - Conference contribution

SN - 978-0-578-40530-8

SP - 2392

EP - 2393

BT - MicroTas 2018

A2 - Tseng, Fan-Gang

A2 - Lee, Gwo-Bin

PB - The Chemical and Biological Microsystems Society

T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018

Y2 - 10 November 2018 through 15 November 2018

ER -

Albers HJ, da Silva Simão JP, Middelkamp HHT, Mummery CL, Passier R , van den Berg A et al. Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices. In Tseng FG, Lee GB, editors, MicroTas 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. The Chemical and Biological Microsystems Society. 2018. p. 2392-2393

Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices

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