Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices

Hugo J. Albers; João P. da Silva Simão; Heleen H.T. Middelkamp; Christine L. Mummery; Robert Passier; Albert  van den Berg; Valeria V. Orlova; Andries D. van der Meer

Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices

Hugo J. Albers
, João P. da Silva Simão
, Heleen H.T. Middelkamp
, Christine L. Mummery
, Robert Passier
, Albert van den Berg
, Valeria V. Orlova
, Andries D. van der Meer

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

A microfluidic thrombosis-on-a-chip platform was developed to compare the pro-thrombotic response of healthy and inflamed monolayers of human umbilical vein endothelial cells (HUVECs) and human induced pluripotent stem cell-derived endothelial cells (hiPSC-ECs). Inflammation was induced by exposing the endothelial cells (ECs) to an inflammatory cytokine Tumor Necrosis Factor-α (TNF-α). After human whole blood perfusion at an arterial shear rate, the platelet coverage and average clot size were determined. Healthy endothelium showed a lower platelet coverage than inflamed endothelium. A minor difference was measured for both platelet coverage and average clot sizes on inflamed HUVECs versus hiPSC-ECs.

Original language	English
Title of host publication	MicroTas 2018
Subtitle of host publication	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences
Editors	Fan-Gang Tseng, Gwo-Bin Lee
Publisher	The Chemical and Biological Microsystems Society
Pages	2392-2393
ISBN (Electronic)	978-1-510-89757-1
ISBN (Print)	978-0-578-40530-8
Publication status	Published - 11 Nov 2018
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018 - Kaohsiung Exhibition Center, Kaohsiung, Taiwan Duration: 10 Nov 2018 → 15 Nov 2018 Conference number: 22 https://cbmsociety.org/conferences/microtas2018/

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018
Abbreviated title	MicroTAS 2018
Country/Territory	Taiwan
City	Kaohsiung
Period	10/11/18 → 15/11/18
Internet address	https://cbmsociety.org/conferences/microtas2018/

Keywords

Thrombosis
Organ-on-a-chip
Vasculature
Human induced pluripotent stem cells
Endothelial cells
Human umbilical vein endothelial cells
Whole blood
Microfluidics

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Albers, H. J., da Silva Simão, J. P., Middelkamp, H. H. T., Mummery, C. L., Passier, R., van den Berg, A., Orlova, V. V., & van der Meer, A. D. (2018). Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices. In F.-G. Tseng, & G.-B. Lee (Eds.), MicroTas 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 2392-2393). The Chemical and Biological Microsystems Society.

Albers, Hugo J. ; da Silva Simão, João P. ; Middelkamp, Heleen H.T. et al. / Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices. MicroTas 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. editor / Fan-Gang Tseng ; Gwo-Bin Lee. The Chemical and Biological Microsystems Society, 2018. pp. 2392-2393

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title = "Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices",

abstract = "A microfluidic thrombosis-on-a-chip platform was developed to compare the pro-thrombotic response of healthy and inflamed monolayers of human umbilical vein endothelial cells (HUVECs) and human induced pluripotent stem cell-derived endothelial cells (hiPSC-ECs). Inflammation was induced by exposing the endothelial cells (ECs) to an inflammatory cytokine Tumor Necrosis Factor-α (TNF-α). After human whole blood perfusion at an arterial shear rate, the platelet coverage and average clot size were determined. Healthy endothelium showed a lower platelet coverage than inflamed endothelium. A minor difference was measured for both platelet coverage and average clot sizes on inflamed HUVECs versus hiPSC-ECs.",

keywords = "Thrombosis, Organ-on-a-chip, Vasculature, Human induced pluripotent stem cells, Endothelial cells, Human umbilical vein endothelial cells, Whole blood, Microfluidics",

author = "Albers, \{Hugo J.\} and \{da Silva Sim{\~a}o\}, \{Jo{\~a}o P.\} and Middelkamp, \{Heleen H.T.\} and Mummery, \{Christine L.\} and Robert Passier and \{van den Berg\}, Albert and Orlova, \{Valeria V.\} and \{van der Meer\}, \{Andries D.\}",

year = "2018",

month = nov,

day = "11",

language = "English",

isbn = "978-0-578-40530-8",

pages = "2392--2393",

editor = "Fan-Gang Tseng and Gwo-Bin Lee",

booktitle = "MicroTas 2018",

publisher = "The Chemical and Biological Microsystems Society",

note = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018, MicroTAS 2018 ; Conference date: 10-11-2018 Through 15-11-2018",

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Albers, HJ, da Silva Simão, JP, Middelkamp, HHT, Mummery, CL, Passier, R , van den Berg, A, Orlova, VV & van der Meer, AD 2018, Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices. in F-G Tseng & G-B Lee (eds), MicroTas 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. The Chemical and Biological Microsystems Society, pp. 2392-2393, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018, Kaohsiung, Taiwan, 10/11/18.

Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices. / Albers, Hugo J.; da Silva Simão, João P.; Middelkamp, Heleen H.T. et al.
MicroTas 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. ed. / Fan-Gang Tseng; Gwo-Bin Lee. The Chemical and Biological Microsystems Society, 2018. p. 2392-2393.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices

AU - Albers, Hugo J.

AU - da Silva Simão, João P.

AU - Middelkamp, Heleen H.T.

AU - Mummery, Christine L.

AU - Passier, Robert

AU - van den Berg, Albert

AU - Orlova, Valeria V.

AU - van der Meer, Andries D.

N1 - Conference code: 22

PY - 2018/11/11

Y1 - 2018/11/11

N2 - A microfluidic thrombosis-on-a-chip platform was developed to compare the pro-thrombotic response of healthy and inflamed monolayers of human umbilical vein endothelial cells (HUVECs) and human induced pluripotent stem cell-derived endothelial cells (hiPSC-ECs). Inflammation was induced by exposing the endothelial cells (ECs) to an inflammatory cytokine Tumor Necrosis Factor-α (TNF-α). After human whole blood perfusion at an arterial shear rate, the platelet coverage and average clot size were determined. Healthy endothelium showed a lower platelet coverage than inflamed endothelium. A minor difference was measured for both platelet coverage and average clot sizes on inflamed HUVECs versus hiPSC-ECs.

AB - A microfluidic thrombosis-on-a-chip platform was developed to compare the pro-thrombotic response of healthy and inflamed monolayers of human umbilical vein endothelial cells (HUVECs) and human induced pluripotent stem cell-derived endothelial cells (hiPSC-ECs). Inflammation was induced by exposing the endothelial cells (ECs) to an inflammatory cytokine Tumor Necrosis Factor-α (TNF-α). After human whole blood perfusion at an arterial shear rate, the platelet coverage and average clot size were determined. Healthy endothelium showed a lower platelet coverage than inflamed endothelium. A minor difference was measured for both platelet coverage and average clot sizes on inflamed HUVECs versus hiPSC-ECs.

KW - Thrombosis

KW - Organ-on-a-chip

KW - Vasculature

KW - Human induced pluripotent stem cells

KW - Endothelial cells

KW - Human umbilical vein endothelial cells

KW - Whole blood

KW - Microfluidics

M3 - Conference contribution

SN - 978-0-578-40530-8

SP - 2392

EP - 2393

BT - MicroTas 2018

A2 - Tseng, Fan-Gang

A2 - Lee, Gwo-Bin

PB - The Chemical and Biological Microsystems Society

T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018

Y2 - 10 November 2018 through 15 November 2018

ER -

Albers HJ, da Silva Simão JP, Middelkamp HHT, Mummery CL, Passier R , van den Berg A et al. Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices. In Tseng FG, Lee GB, editors, MicroTas 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. The Chemical and Biological Microsystems Society. 2018. p. 2392-2393

Human induced pluripotent stem cell-derived endothelial cells in thrombosis-on-a-chip devices

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