Hyaluronan-based antiadhesive membrane has no major effect on intraperitoneal growth of colonic tumour cells

C.J.J.M. Sikkink; V.A. Postma; M.M.P.J. Reijnen; B. de Man; R.P. Bleichrodt; H. van Goor

doi:10.1159/000076653

Hyaluronan-based antiadhesive membrane has no major effect on intraperitoneal growth of colonic tumour cells

C.J.J.M. Sikkink, V.A. Postma, M.M.P.J. Reijnen, B. de Man, R.P. Bleichrodt, H. van Goor^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

7 Citations (Scopus)

Abstract

Background: A relationship between post-surgical adhesion formation and peritoneal tumour implantation has been proposed. Hyaluronan (HA)-based agents reduce adhesion formation, but the effect on peritoneal tumour is not established. This study investigated the influence of a HA-containing agent on intraperitoneal tumour in an experimental model.

Methods: 66 Balb/c mice underwent laparotomy and damage was inflicted to the parietal peritoneum. The animals were randomized into five groups. Groups 1 and 2 received HA-carboxymethylcellulose bioresorbable membrane and no treatment, respectively. Mice in groups 3-5 were injected intraperitoneally with 10⁵ colon 26-B cells after the laparotomy. Treatment consisted of HA membrane, no HA agent and placement of HA membrane on the non-traumatized peritoneal wall, respectively. Animals were killed after 14 days; adhesions were scored in groups 1 and 2, and the tumour mass in groups 3-5. 45 Wag/Rij rats underwent the same procedures and treatment as mice in groups 3-5. In rats, 10 ⁶ CC-531 cells were injected. Rats were killed after 3 weeks and the tumour mass was scored.

Results: HA membrane resulted in a significant reduction of adhesions, but had no major effect on the intraperitoneal tumour mass in mice and rats.

Conclusion: HA-carboxymethylcellulose bioresorbable membrane has no major effect on intraperitoneal tumour implantation and growth in an experimental model.

Original language	English
Pages (from-to)	123-128
Number of pages	6
Journal	European Surgical Research
Volume	36
Issue number	2
DOIs	https://doi.org/10.1159/000076653
Publication status	Published - 23 Mar 2004
Externally published	Yes

Keywords

Adhesions
Hyaluronan
Peritoneal tumour implantation
Peritoneum
Tumour, Balb/c mouse model
Tumour, WAG/Rij rat model

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title = "Hyaluronan-based antiadhesive membrane has no major effect on intraperitoneal growth of colonic tumour cells",

abstract = "Background: A relationship between post-surgical adhesion formation and peritoneal tumour implantation has been proposed. Hyaluronan (HA)-based agents reduce adhesion formation, but the effect on peritoneal tumour is not established. This study investigated the influence of a HA-containing agent on intraperitoneal tumour in an experimental model.Methods: 66 Balb/c mice underwent laparotomy and damage was inflicted to the parietal peritoneum. The animals were randomized into five groups. Groups 1 and 2 received HA-carboxymethylcellulose bioresorbable membrane and no treatment, respectively. Mice in groups 3-5 were injected intraperitoneally with 105 colon 26-B cells after the laparotomy. Treatment consisted of HA membrane, no HA agent and placement of HA membrane on the non-traumatized peritoneal wall, respectively. Animals were killed after 14 days; adhesions were scored in groups 1 and 2, and the tumour mass in groups 3-5. 45 Wag/Rij rats underwent the same procedures and treatment as mice in groups 3-5. In rats, 10 6 CC-531 cells were injected. Rats were killed after 3 weeks and the tumour mass was scored.Results: HA membrane resulted in a significant reduction of adhesions, but had no major effect on the intraperitoneal tumour mass in mice and rats.Conclusion: HA-carboxymethylcellulose bioresorbable membrane has no major effect on intraperitoneal tumour implantation and growth in an experimental model.",

keywords = "Adhesions, Hyaluronan, Peritoneal tumour implantation, Peritoneum, Tumour, Balb/c mouse model, Tumour, WAG/Rij rat model",

author = "C.J.J.M. Sikkink and V.A. Postma and M.M.P.J. Reijnen and {de Man}, B. and R.P. Bleichrodt and {van Goor}, H.",

year = "2004",

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doi = "10.1159/000076653",

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T1 - Hyaluronan-based antiadhesive membrane has no major effect on intraperitoneal growth of colonic tumour cells

AU - Sikkink, C.J.J.M.

AU - Postma, V.A.

AU - Reijnen, M.M.P.J.

AU - de Man, B.

AU - Bleichrodt, R.P.

AU - van Goor, H.

PY - 2004/3/23

Y1 - 2004/3/23

N2 - Background: A relationship between post-surgical adhesion formation and peritoneal tumour implantation has been proposed. Hyaluronan (HA)-based agents reduce adhesion formation, but the effect on peritoneal tumour is not established. This study investigated the influence of a HA-containing agent on intraperitoneal tumour in an experimental model.Methods: 66 Balb/c mice underwent laparotomy and damage was inflicted to the parietal peritoneum. The animals were randomized into five groups. Groups 1 and 2 received HA-carboxymethylcellulose bioresorbable membrane and no treatment, respectively. Mice in groups 3-5 were injected intraperitoneally with 105 colon 26-B cells after the laparotomy. Treatment consisted of HA membrane, no HA agent and placement of HA membrane on the non-traumatized peritoneal wall, respectively. Animals were killed after 14 days; adhesions were scored in groups 1 and 2, and the tumour mass in groups 3-5. 45 Wag/Rij rats underwent the same procedures and treatment as mice in groups 3-5. In rats, 10 6 CC-531 cells were injected. Rats were killed after 3 weeks and the tumour mass was scored.Results: HA membrane resulted in a significant reduction of adhesions, but had no major effect on the intraperitoneal tumour mass in mice and rats.Conclusion: HA-carboxymethylcellulose bioresorbable membrane has no major effect on intraperitoneal tumour implantation and growth in an experimental model.

AB - Background: A relationship between post-surgical adhesion formation and peritoneal tumour implantation has been proposed. Hyaluronan (HA)-based agents reduce adhesion formation, but the effect on peritoneal tumour is not established. This study investigated the influence of a HA-containing agent on intraperitoneal tumour in an experimental model.Methods: 66 Balb/c mice underwent laparotomy and damage was inflicted to the parietal peritoneum. The animals were randomized into five groups. Groups 1 and 2 received HA-carboxymethylcellulose bioresorbable membrane and no treatment, respectively. Mice in groups 3-5 were injected intraperitoneally with 105 colon 26-B cells after the laparotomy. Treatment consisted of HA membrane, no HA agent and placement of HA membrane on the non-traumatized peritoneal wall, respectively. Animals were killed after 14 days; adhesions were scored in groups 1 and 2, and the tumour mass in groups 3-5. 45 Wag/Rij rats underwent the same procedures and treatment as mice in groups 3-5. In rats, 10 6 CC-531 cells were injected. Rats were killed after 3 weeks and the tumour mass was scored.Results: HA membrane resulted in a significant reduction of adhesions, but had no major effect on the intraperitoneal tumour mass in mice and rats.Conclusion: HA-carboxymethylcellulose bioresorbable membrane has no major effect on intraperitoneal tumour implantation and growth in an experimental model.

KW - Adhesions

KW - Hyaluronan

KW - Peritoneal tumour implantation

KW - Peritoneum

KW - Tumour, Balb/c mouse model

KW - Tumour, WAG/Rij rat model

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SN - 0014-312X

VL - 36

SP - 123

EP - 128

JO - European Surgical Research

JF - European Surgical Research

IS - 2

ER -

Hyaluronan-based antiadhesive membrane has no major effect on intraperitoneal growth of colonic tumour cells

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Keywords

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