Hydrogel/poly-dimethylsiloxane hybrid bioreactor facilitating 3D cell culturing

Bart Schurink, Regina Luttge

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)
18 Downloads (Pure)

Abstract

The authors present a hydrogel/poly-dimethylsiloxane (PDMS) hybrid bioreactor. The bioreactor enables a low shear stress 3D culture by integrating a hydrogel as a barrier into a PDMS casing. The use of PDMS allows the reversible adhesion of the device to a commercially available microelectrode array. A two-step molding process facilitates this relatively simple, cost effective, and leakage-free add-on microculture system. Agarose (2%) is used as hydrogel barrier material and mass transport is evaluated by fluorescein isothiocyanate-albumin fluorescence under static conditions which yields a diffusion coefficient of average value of 2.2 × 10-7 cm 2 s-1 across the barrier. To validate our bioreactor for diffusion limited 3D cell culture, rat cortical cells were successfully cultured in Matrigel for 6 days.

Original languageEnglish
Article number6F903
Number of pages5
JournalJournal of vacuum science and technology. B: Microelectronics and nanometer structures
Volume31
Issue number6
DOIs
Publication statusPublished - Nov 2013

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