Hyperspectral coherent anti-Stokes Raman scattering microscopy for in situ analysis of solid-state crystal polymorphs

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

1 Citation (Scopus)

Abstract

Hyperspectral coherent anti-Stokes Raman scattering (CARS) microscopy is quickly becoming a prominent imaging modality because of its many advantages over the traditional paradigm of multispectral CARS. In particular, recording a significant portion of the vibrational spectrum at each spatial pixel allows image-wide spectral analysis at much higher rates than can be achieved with spontaneous Raman. We recently developed a hyperspectral CARS method, the driving principle behind which is the fast acquisition and display of a hyperspectral datacube as a set of intuitive images wherein each material in a sample appears with a unique trio of colors. Here we use this system to image and analyze two types of polymorphic samples: the pseudopolymorphic hydration of theophylline, and the packing polymorphs of the sugar alcohol mannitol. In addition to these solid-state form modifications we have observed spectral variations of crystalline mannitol and diprophylline as functions of their orientations relative to the optical fields. We use that information to visualize the distributions of these compounds in a pharmaceutical solid oral dosage form.
Original languageEnglish
Title of host publicationProceedings of Multiphoton Microscopy in the Biomedical Sciences XIII
EditorsA. Periasami, K. Konig, P.T.C. So
Place of PublicationSan Francisco, CA, USA
PublisherSPIE
Pages-
DOIs
Publication statusPublished - 3 Feb 2013

Publication series

NameProceedings of SPIE
PublisherSPIE
Volume8588
ISSN (Print)0277-786X

Fingerprint

mannitol
Raman spectra
microscopy
solid state
crystals
sugars
vibrational spectra
spectrum analysis
hydration
acquisition
alcohols
recording
pixels
color
dosage

Keywords

  • METIS-297582
  • IR-87256

Cite this

Garbacik, E. T., Fussell, A. L., Gures, S., Korterik, J. P., Otto, C., Herek, J. L., & Offerhaus, H. L. (2013). Hyperspectral coherent anti-Stokes Raman scattering microscopy for in situ analysis of solid-state crystal polymorphs. In A. Periasami, K. Konig, & P. T. C. So (Eds.), Proceedings of Multiphoton Microscopy in the Biomedical Sciences XIII (pp. -). (Proceedings of SPIE; Vol. 8588). San Francisco, CA, USA: SPIE. https://doi.org/10.1117/12.2004522
Garbacik, E.T. ; Fussell, A.L. ; Gures, S. ; Korterik, Jeroen P. ; Otto, Cornelis ; Herek, Jennifer Lynn ; Offerhaus, Herman L. / Hyperspectral coherent anti-Stokes Raman scattering microscopy for in situ analysis of solid-state crystal polymorphs. Proceedings of Multiphoton Microscopy in the Biomedical Sciences XIII. editor / A. Periasami ; K. Konig ; P.T.C. So. San Francisco, CA, USA : SPIE, 2013. pp. - (Proceedings of SPIE).
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abstract = "Hyperspectral coherent anti-Stokes Raman scattering (CARS) microscopy is quickly becoming a prominent imaging modality because of its many advantages over the traditional paradigm of multispectral CARS. In particular, recording a significant portion of the vibrational spectrum at each spatial pixel allows image-wide spectral analysis at much higher rates than can be achieved with spontaneous Raman. We recently developed a hyperspectral CARS method, the driving principle behind which is the fast acquisition and display of a hyperspectral datacube as a set of intuitive images wherein each material in a sample appears with a unique trio of colors. Here we use this system to image and analyze two types of polymorphic samples: the pseudopolymorphic hydration of theophylline, and the packing polymorphs of the sugar alcohol mannitol. In addition to these solid-state form modifications we have observed spectral variations of crystalline mannitol and diprophylline as functions of their orientations relative to the optical fields. We use that information to visualize the distributions of these compounds in a pharmaceutical solid oral dosage form.",
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Garbacik, ET, Fussell, AL, Gures, S, Korterik, JP, Otto, C, Herek, JL & Offerhaus, HL 2013, Hyperspectral coherent anti-Stokes Raman scattering microscopy for in situ analysis of solid-state crystal polymorphs. in A Periasami, K Konig & PTC So (eds), Proceedings of Multiphoton Microscopy in the Biomedical Sciences XIII. Proceedings of SPIE, vol. 8588, SPIE, San Francisco, CA, USA, pp. -. https://doi.org/10.1117/12.2004522

Hyperspectral coherent anti-Stokes Raman scattering microscopy for in situ analysis of solid-state crystal polymorphs. / Garbacik, E.T.; Fussell, A.L.; Gures, S.; Korterik, Jeroen P.; Otto, Cornelis; Herek, Jennifer Lynn; Offerhaus, Herman L.

Proceedings of Multiphoton Microscopy in the Biomedical Sciences XIII. ed. / A. Periasami; K. Konig; P.T.C. So. San Francisco, CA, USA : SPIE, 2013. p. - (Proceedings of SPIE; Vol. 8588).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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T1 - Hyperspectral coherent anti-Stokes Raman scattering microscopy for in situ analysis of solid-state crystal polymorphs

AU - Garbacik, E.T.

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AU - Gures, S.

AU - Korterik, Jeroen P.

AU - Otto, Cornelis

AU - Herek, Jennifer Lynn

AU - Offerhaus, Herman L.

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N2 - Hyperspectral coherent anti-Stokes Raman scattering (CARS) microscopy is quickly becoming a prominent imaging modality because of its many advantages over the traditional paradigm of multispectral CARS. In particular, recording a significant portion of the vibrational spectrum at each spatial pixel allows image-wide spectral analysis at much higher rates than can be achieved with spontaneous Raman. We recently developed a hyperspectral CARS method, the driving principle behind which is the fast acquisition and display of a hyperspectral datacube as a set of intuitive images wherein each material in a sample appears with a unique trio of colors. Here we use this system to image and analyze two types of polymorphic samples: the pseudopolymorphic hydration of theophylline, and the packing polymorphs of the sugar alcohol mannitol. In addition to these solid-state form modifications we have observed spectral variations of crystalline mannitol and diprophylline as functions of their orientations relative to the optical fields. We use that information to visualize the distributions of these compounds in a pharmaceutical solid oral dosage form.

AB - Hyperspectral coherent anti-Stokes Raman scattering (CARS) microscopy is quickly becoming a prominent imaging modality because of its many advantages over the traditional paradigm of multispectral CARS. In particular, recording a significant portion of the vibrational spectrum at each spatial pixel allows image-wide spectral analysis at much higher rates than can be achieved with spontaneous Raman. We recently developed a hyperspectral CARS method, the driving principle behind which is the fast acquisition and display of a hyperspectral datacube as a set of intuitive images wherein each material in a sample appears with a unique trio of colors. Here we use this system to image and analyze two types of polymorphic samples: the pseudopolymorphic hydration of theophylline, and the packing polymorphs of the sugar alcohol mannitol. In addition to these solid-state form modifications we have observed spectral variations of crystalline mannitol and diprophylline as functions of their orientations relative to the optical fields. We use that information to visualize the distributions of these compounds in a pharmaceutical solid oral dosage form.

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Garbacik ET, Fussell AL, Gures S, Korterik JP, Otto C, Herek JL et al. Hyperspectral coherent anti-Stokes Raman scattering microscopy for in situ analysis of solid-state crystal polymorphs. In Periasami A, Konig K, So PTC, editors, Proceedings of Multiphoton Microscopy in the Biomedical Sciences XIII. San Francisco, CA, USA: SPIE. 2013. p. -. (Proceedings of SPIE). https://doi.org/10.1117/12.2004522