Hyperspectral coherent anti-Stokes Raman scattering microscopy for in situ analysis of solid-state crystal polymorphs

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Abstract

Hyperspectral coherent anti-Stokes Raman scattering (CARS) microscopy is quickly becoming a prominent imaging modality because of its many advantages over the traditional paradigm of multispectral CARS. In particular, recording a significant portion of the vibrational spectrum at each spatial pixel allows image-wide spectral analysis at much higher rates than can be achieved with spontaneous Raman. We recently developed a hyperspectral CARS method, the driving principle behind which is the fast acquisition and display of a hyperspectral datacube as a set of intuitive images wherein each material in a sample appears with a unique trio of colors. Here we use this system to image and analyze two types of polymorphic samples: the pseudopolymorphic hydration of theophylline, and the packing polymorphs of the sugar alcohol mannitol. In addition to these solid-state form modifications we have observed spectral variations of crystalline mannitol and diprophylline as functions of their orientations relative to the optical fields. We use that information to visualize the distributions of these compounds in a pharmaceutical solid oral dosage form.
Original languageEnglish
Title of host publicationProceedings of Multiphoton Microscopy in the Biomedical Sciences XIII
EditorsA. Periasami, K. Konig, P.T.C. So
Place of PublicationSan Francisco, CA, USA
PublisherSPIE
Pages-
DOIs
Publication statusPublished - 3 Feb 2013

Publication series

NameProceedings of SPIE
PublisherSPIE
Volume8588
ISSN (Print)0277-786X

Keywords

  • METIS-297582
  • IR-87256

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    Garbacik, E. T., Fussell, A. L., Gures, S., Korterik, J. P., Otto, C., Herek, J. L., & Offerhaus, H. L. (2013). Hyperspectral coherent anti-Stokes Raman scattering microscopy for in situ analysis of solid-state crystal polymorphs. In A. Periasami, K. Konig, & P. T. C. So (Eds.), Proceedings of Multiphoton Microscopy in the Biomedical Sciences XIII (pp. -). (Proceedings of SPIE; Vol. 8588). San Francisco, CA, USA: SPIE. https://doi.org/10.1117/12.2004522