Hyperspectral imaging system for imaging O2Hb and HHb concentration changes in tissue for various clinical applications

John H G M Klaessens, Rowland De Roode, Rudolf M. Verdaasdonk, Herke J. Noordmans

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

10 Citations (Scopus)
27 Downloads (Pure)

Abstract

To observe local variations in temperature, oxygenation and blood perfusion over time, four imaging systems were developed and compared: Two systems consisting of white broadband light source and a CCD camera in combination with a Liquid Crystal Tunable Filter, one in the visual domain, 420-730 nm, and one in the infrared domain, 650-1100 nm. Thirdly, a CCD camera in combination with a software controlled hyper-spectral light source consisting of a panel with 600 LEDs divided in 17 spectral groups in the range from 370 to 880 nm so that specific spectral distributions can be generated at high repetition rate (>1000 Hz) and, fourthly a standard IR thermal camera for comparison. From the acquired images at the selected wavelengths chromophores concentration images of oxy and deoxy hemoglobin can be calculated applying different algorithms. These imaging techniques were applied and compared for various clinical applications: Tumor demarcation, early inflammation, effectiveness of peripheral nerve block anesthesia, and localization of epileptic seizure. The relative changes in oxygenation and temperature could be clearly observed in good correlation with the physiological condition. The algorithms and data collection/processing can be optimized to enable a real-time diagnostic technique.

Original languageEnglish
Title of host publicationAdvanced Biomedical and Clinical Diagnostic Systems IX
DOIs
Publication statusPublished - 2 May 2011
Externally publishedYes
EventAdvanced Biomedical and Clinical Diagnostic Systems IX 2011 - San Francisco, United States
Duration: 23 Jan 201125 Jan 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7890
ISSN (Print)1605-7422

Conference

ConferenceAdvanced Biomedical and Clinical Diagnostic Systems IX 2011
Country/TerritoryUnited States
CitySan Francisco
Period23/01/1125/01/11

Keywords

  • 2D
  • Hemoglobin
  • IR Thermography
  • Near Infrared
  • Oxygen
  • Spectroscopy

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