The physical adsorption and chemical immobilization of Cancanavalin A (Con A) on semiconductor surfaces SiO2 and Ta2O5 are investigated with the help of radioactive-labelled Con A. It is shown that Con A easily forms multi-layered systems and that SiO2 has preference for the non-labelled protein. Surface coverage by the two methods is compared with respect to stability to mechanical stress and clearly shows the drawback of physical adsorption. In addition, the complexing abilities of Con A for mannose are investigated showing 50% activity compared to Con A in solution for monolayer systems, increasing to 75% for multi-layered systems.
Kremer, F. J. B., Engbersen, J. F. J., Kruise, J., Bergveld, P., Starmans, D. A. J., Feijen, J., & Reinhoudt, D. N. (1993). Immobilization and activity of concanavalin A on tantalum pentoxide and silicon dioxide surfaces. Sensors and actuators. B: Chemical, 13(1-3), 176-179. https://doi.org/10.1016/0925-4005(93)85355-E