Immunocapturing of extracellular vesicles on stainless steel for multi-modal individual characterization with correlative light, electron and probe microscopy

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Abstract

Here, we report a robust platform for multi-modal analysis of immuno-captured individual extracellular vesicles (EVs). Stainless steel substrates were surface-modified to covalently immobilize specific antibodies targeting proteins found on EVs. Using PDMS microchannels, EVs were selectively captured on the substrates. Next, individual EVs were retraced and correlatively characterized here using SEM, AFM and Raman Spectroscopy.
Original languageEnglish
Title of host publicationMicroTas 2018
Subtitle of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences
EditorsFan-Gang Tseng, Gwo-Bin Lee
PublisherThe Chemical and Biological Microsystems Society
Pages2402-2403
Number of pages2
ISBN (Electronic)978-1-510-89757-1
ISBN (Print)978-0-578-40530-8
Publication statusPublished - 11 Nov 2018
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018 - Kaohsiung Exhibition Center, Kaohsiung, Taiwan, Province of China
Duration: 10 Nov 201815 Nov 2018
Conference number: 22
https://cbmsociety.org/conferences/microtas2018/

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018
Abbreviated titleMicroTAS 2018
CountryTaiwan, Province of China
CityKaohsiung
Period10/11/1815/11/18
Internet address

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Keywords

  • Extracellular vesicles
  • AFM
  • SEM
  • Raman
  • Correlative light
  • Electron and probe microscopy

Cite this

Beekman, P., Enciso Martinez, A., Terstappen, L., Otto, C., & le Gac, S. (2018). Immunocapturing of extracellular vesicles on stainless steel for multi-modal individual characterization with correlative light, electron and probe microscopy. In F-G. Tseng, & G-B. Lee (Eds.), MicroTas 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 2402-2403). The Chemical and Biological Microsystems Society.