Immunogold labels: cell-surface markers in atomic force microscopy

Constant A.J. Putman, Bart G. de Grooth, Paul K. Hansma, Niek F. van Hulst, Jan Greve

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Abstract

The feasibility of using immunogold labels as cell-surface markers in atomic force microscopy is shown in this paper. The atomic force microscope (AFM) was used to image the surface of immunogold-labeled human lymphocytes. The lymphocytes were isolated from whole blood and labeled by an indirect immunolabeling method using the monoclonal antibody anti-CD3 and a secondary antibody (Goat-anti-Mouse) linked to 30 nm colloidal gold particles. Some of the samples were enhanced by silver deposition o­nto the gold particles. The AFM images reveal the colloidal gold particles o­n the cell surface, with and without silver enhancement. Individual immunogold (-silver) particles are clearly resolved from the cell surface thus determining the location of antigens. The 30 nm gold particles appear in the AFM images having an average size of about 80 nm due to convolution between gold particle and AFM tip.
Original languageEnglish
Pages (from-to)177-182
Number of pages6
JournalUltramicroscopy
Volume48
Issue number1-2
DOIs
Publication statusPublished - 1993

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Atomic force microscopy
Microscopes
Gold
Silver
atomic force microscopy
Gold Colloid
Lymphocytes
gold
cells
microscopes
lymphocytes
silver
antibodies
Convolution
Monoclonal antibodies
goats
Antigens
Blood

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Putman, C. A. J., de Grooth, B. G., Hansma, P. K., van Hulst, N. F., & Greve, J. (1993). Immunogold labels: cell-surface markers in atomic force microscopy. Ultramicroscopy, 48(1-2), 177-182. https://doi.org/10.1016/0304-3991(93)90180-6
Putman, Constant A.J. ; de Grooth, Bart G. ; Hansma, Paul K. ; van Hulst, Niek F. ; Greve, Jan. / Immunogold labels : cell-surface markers in atomic force microscopy. In: Ultramicroscopy. 1993 ; Vol. 48, No. 1-2. pp. 177-182.
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Putman, CAJ, de Grooth, BG, Hansma, PK, van Hulst, NF & Greve, J 1993, 'Immunogold labels: cell-surface markers in atomic force microscopy' Ultramicroscopy, vol. 48, no. 1-2, pp. 177-182. https://doi.org/10.1016/0304-3991(93)90180-6

Immunogold labels : cell-surface markers in atomic force microscopy. / Putman, Constant A.J.; de Grooth, Bart G.; Hansma, Paul K.; van Hulst, Niek F.; Greve, Jan.

In: Ultramicroscopy, Vol. 48, No. 1-2, 1993, p. 177-182.

Research output: Contribution to journalArticleAcademicpeer-review

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AB - The feasibility of using immunogold labels as cell-surface markers in atomic force microscopy is shown in this paper. The atomic force microscope (AFM) was used to image the surface of immunogold-labeled human lymphocytes. The lymphocytes were isolated from whole blood and labeled by an indirect immunolabeling method using the monoclonal antibody anti-CD3 and a secondary antibody (Goat-anti-Mouse) linked to 30 nm colloidal gold particles. Some of the samples were enhanced by silver deposition o­nto the gold particles. The AFM images reveal the colloidal gold particles o­n the cell surface, with and without silver enhancement. Individual immunogold (-silver) particles are clearly resolved from the cell surface thus determining the location of antigens. The 30 nm gold particles appear in the AFM images having an average size of about 80 nm due to convolution between gold particle and AFM tip.

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Putman CAJ, de Grooth BG, Hansma PK, van Hulst NF, Greve J. Immunogold labels: cell-surface markers in atomic force microscopy. Ultramicroscopy. 1993;48(1-2):177-182. https://doi.org/10.1016/0304-3991(93)90180-6