Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 177-182 |
| Number of pages | 6 |
| Journal | Ultramicroscopy |
| Volume | 48 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 1993 |
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Immunogold labels : cell-surface markers in atomic force microscopy. / Putman, Constant A.J.; de Grooth, Bart G.; Hansma, Paul K.; van Hulst, Niek F.; Greve, Jan.
In: Ultramicroscopy, Vol. 48, No. 1-2, 1993, p. 177-182.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Immunogold labels
T2 - cell-surface markers in atomic force microscopy
AU - Putman, Constant A.J.
AU - de Grooth, Bart G.
AU - Hansma, Paul K.
AU - van Hulst, Niek F.
AU - Greve, Jan
PY - 1993
Y1 - 1993
N2 - The feasibility of using immunogold labels as cell-surface markers in atomic force microscopy is shown in this paper. The atomic force microscope (AFM) was used to image the surface of immunogold-labeled human lymphocytes. The lymphocytes were isolated from whole blood and labeled by an indirect immunolabeling method using the monoclonal antibody anti-CD3 and a secondary antibody (Goat-anti-Mouse) linked to 30 nm colloidal gold particles. Some of the samples were enhanced by silver deposition onto the gold particles. The AFM images reveal the colloidal gold particles on the cell surface, with and without silver enhancement. Individual immunogold (-silver) particles are clearly resolved from the cell surface thus determining the location of antigens. The 30 nm gold particles appear in the AFM images having an average size of about 80 nm due to convolution between gold particle and AFM tip.
AB - The feasibility of using immunogold labels as cell-surface markers in atomic force microscopy is shown in this paper. The atomic force microscope (AFM) was used to image the surface of immunogold-labeled human lymphocytes. The lymphocytes were isolated from whole blood and labeled by an indirect immunolabeling method using the monoclonal antibody anti-CD3 and a secondary antibody (Goat-anti-Mouse) linked to 30 nm colloidal gold particles. Some of the samples were enhanced by silver deposition onto the gold particles. The AFM images reveal the colloidal gold particles on the cell surface, with and without silver enhancement. Individual immunogold (-silver) particles are clearly resolved from the cell surface thus determining the location of antigens. The 30 nm gold particles appear in the AFM images having an average size of about 80 nm due to convolution between gold particle and AFM tip.
U2 - 10.1016/0304-3991(93)90180-6
DO - 10.1016/0304-3991(93)90180-6
M3 - Article
VL - 48
SP - 177
EP - 182
JO - Ultramicroscopy
JF - Ultramicroscopy
SN - 0304-3991
IS - 1-2
ER -