Impedance sensing for monitoring neuronal coverage and comparison with microscopy

Remy Wiertz, Wim Rutten, Enrico Marani

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    We investigated the applicability of electric impedance sensing (IS) to monitor the coverage of adhered dissociated neuronal cells on glass substrates with embedded electrodes. IS is a sensitive method for the quantification of changes in cell morphology and cell mobility, making it suitable to study aggregation kinetics. Various sizes of electrodes were compared for the real-time recording of the impedance of adhering cells, at eight frequencies (range: 5 Hz-20 kHz). The real part of the impedance showed to be most sensitive at frequencies of 10 and 20 kHz for the two largest electrodes (7850 and 125 600 μm2). Compared to simultaneous microscopic evaluation of cell coverage and cell spreading, IS shows more detail.
    Original languageUndefined
    Pages (from-to)2379-2385
    Number of pages7
    JournalIEEE transactions on biomedical engineering
    Issue number10
    Publication statusPublished - Oct 2010


    • BSS-Neurotechnology and cellular engineering
    • electric impedance sensing (IS)
    • neuronal cell coverage
    • Aggre- gation
    • EWI-18652
    • METIS-271092
    • IR-74054
    • neuronal cultures

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