Impedance sensing for monitoring neuronal coverage and comparison with microscopy

Remy Wiertz; Wim Rutten; Enrico Marani

doi:10.1109/TBME.2010.2055052

Impedance sensing for monitoring neuronal coverage and comparison with microscopy

Remy Wiertz, Wim Rutten, Enrico Marani

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Abstract

We investigated the applicability of electric impedance sensing (IS) to monitor the coverage of adhered dissociated neuronal cells on glass substrates with embedded electrodes. IS is a sensitive method for the quantification of changes in cell morphology and cell mobility, making it suitable to study aggregation kinetics. Various sizes of electrodes were compared for the real-time recording of the impedance of adhering cells, at eight frequencies (range: 5 Hz-20 kHz). The real part of the impedance showed to be most sensitive at frequencies of 10 and 20 kHz for the two largest electrodes (7850 and 125 600 μm2). Compared to simultaneous microscopic evaluation of cell coverage and cell spreading, IS shows more detail.

Original language	Undefined
Pages (from-to)	2379-2385
Number of pages	7
Journal	IEEE transactions on biomedical engineering
Volume	57
Issue number	10
DOIs	https://doi.org/10.1109/TBME.2010.2055052
Publication status	Published - Oct 2010

Keywords

BSS-Neurotechnology and cellular engineering
electric impedance sensing (IS)
neuronal cell coverage
Aggre- gation
EWI-18652
METIS-271092
IR-74054
neuronal cultures

Access to Document

10.1109/TBME.2010.2055052

Cite this

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title = "Impedance sensing for monitoring neuronal coverage and comparison with microscopy",

abstract = "We investigated the applicability of electric impedance sensing (IS) to monitor the coverage of adhered dissociated neuronal cells on glass substrates with embedded electrodes. IS is a sensitive method for the quantification of changes in cell morphology and cell mobility, making it suitable to study aggregation kinetics. Various sizes of electrodes were compared for the real-time recording of the impedance of adhering cells, at eight frequencies (range: 5 Hz-20 kHz). The real part of the impedance showed to be most sensitive at frequencies of 10 and 20 kHz for the two largest electrodes (7850 and 125 600 μm2). Compared to simultaneous microscopic evaluation of cell coverage and cell spreading, IS shows more detail.",

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