Improved precision using internal standards in disposable point-of-care capillary electrophoresis devices

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    Abstract

    This paper presents a systematic investigation of the viability of using internal standards to correct for chip-to-chip imprecision in disposable microchip capillary electrophoresis (μCE) devices. The effects of two internal standards (CsCl and LiCl) on the reproducibility of Na quantification were explored using in-house fabricated microchips. Five concentrations of NaCl were analyzed consecutively over five days, resulting in a dataset of 900 electropherograms with which to draw reliable conclusions from. We find internal standards can strongly improve the precision of disposable devices.
    Original languageUndefined
    Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
    Place of PublicationSan Diego
    PublisherChemical and Biochemical Society
    Pages2405-2407
    Number of pages3
    ISBN (Print)978-0-9798064-7-6
    Publication statusPublished - 26 Oct 2014
    Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014 - San Antonio, United States
    Duration: 26 Oct 201430 Oct 2014
    Conference number: 18

    Publication series

    NameMicroTAS 2014
    PublisherChemical and Biochemical Society
    ISSN (Print)1556-5904

    Conference

    Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014
    Abbreviated titleMicroTAS
    CountryUnited States
    CitySan Antonio
    Period26/10/1430/10/14

    Keywords

    • EWI-25609
    • METIS-309841
    • IR-93841

    Cite this

    Bidulock, A. C. E., van den Berg, A., & Eijkel, J. C. T. (2014). Improved precision using internal standards in disposable point-of-care capillary electrophoresis devices. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 2405-2407). (MicroTAS 2014). San Diego: Chemical and Biochemical Society.
    Bidulock, A.C.E. ; van den Berg, Albert ; Eijkel, Jan C.T. / Improved precision using internal standards in disposable point-of-care capillary electrophoresis devices. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. San Diego : Chemical and Biochemical Society, 2014. pp. 2405-2407 (MicroTAS 2014).
    @inproceedings{c7af3ae8225d46fd83ddb79a7f158298,
    title = "Improved precision using internal standards in disposable point-of-care capillary electrophoresis devices",
    abstract = "This paper presents a systematic investigation of the viability of using internal standards to correct for chip-to-chip imprecision in disposable microchip capillary electrophoresis (μCE) devices. The effects of two internal standards (CsCl and LiCl) on the reproducibility of Na quantification were explored using in-house fabricated microchips. Five concentrations of NaCl were analyzed consecutively over five days, resulting in a dataset of 900 electropherograms with which to draw reliable conclusions from. We find internal standards can strongly improve the precision of disposable devices.",
    keywords = "EWI-25609, METIS-309841, IR-93841",
    author = "A.C.E. Bidulock and {van den Berg}, Albert and Eijkel, {Jan C.T.}",
    note = "eemcs-eprint-25609",
    year = "2014",
    month = "10",
    day = "26",
    language = "Undefined",
    isbn = "978-0-9798064-7-6",
    series = "MicroTAS 2014",
    publisher = "Chemical and Biochemical Society",
    pages = "2405--2407",
    booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

    }

    Bidulock, ACE, van den Berg, A & Eijkel, JCT 2014, Improved precision using internal standards in disposable point-of-care capillary electrophoresis devices. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. MicroTAS 2014, Chemical and Biochemical Society, San Diego, pp. 2405-2407, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014, San Antonio, United States, 26/10/14.

    Improved precision using internal standards in disposable point-of-care capillary electrophoresis devices. / Bidulock, A.C.E.; van den Berg, Albert; Eijkel, Jan C.T.

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. San Diego : Chemical and Biochemical Society, 2014. p. 2405-2407 (MicroTAS 2014).

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    TY - GEN

    T1 - Improved precision using internal standards in disposable point-of-care capillary electrophoresis devices

    AU - Bidulock, A.C.E.

    AU - van den Berg, Albert

    AU - Eijkel, Jan C.T.

    N1 - eemcs-eprint-25609

    PY - 2014/10/26

    Y1 - 2014/10/26

    N2 - This paper presents a systematic investigation of the viability of using internal standards to correct for chip-to-chip imprecision in disposable microchip capillary electrophoresis (μCE) devices. The effects of two internal standards (CsCl and LiCl) on the reproducibility of Na quantification were explored using in-house fabricated microchips. Five concentrations of NaCl were analyzed consecutively over five days, resulting in a dataset of 900 electropherograms with which to draw reliable conclusions from. We find internal standards can strongly improve the precision of disposable devices.

    AB - This paper presents a systematic investigation of the viability of using internal standards to correct for chip-to-chip imprecision in disposable microchip capillary electrophoresis (μCE) devices. The effects of two internal standards (CsCl and LiCl) on the reproducibility of Na quantification were explored using in-house fabricated microchips. Five concentrations of NaCl were analyzed consecutively over five days, resulting in a dataset of 900 electropherograms with which to draw reliable conclusions from. We find internal standards can strongly improve the precision of disposable devices.

    KW - EWI-25609

    KW - METIS-309841

    KW - IR-93841

    M3 - Conference contribution

    SN - 978-0-9798064-7-6

    T3 - MicroTAS 2014

    SP - 2405

    EP - 2407

    BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

    PB - Chemical and Biochemical Society

    CY - San Diego

    ER -

    Bidulock ACE, van den Berg A, Eijkel JCT. Improved precision using internal standards in disposable point-of-care capillary electrophoresis devices. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. San Diego: Chemical and Biochemical Society. 2014. p. 2405-2407. (MicroTAS 2014).