Improved precision using internal standards in disposable point-of-care capillary electrophoresis devices

A.C.E. Bidulock; Albert van den Berg; Jan C.T. Eijkel

Improved precision using internal standards in disposable point-of-care capillary electrophoresis devices

A.C.E. Bidulock, Albert van den Berg, Jan C.T. Eijkel

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

This paper presents a systematic investigation of the viability of using internal standards to correct for chip-to-chip imprecision in disposable microchip capillary electrophoresis (μCE) devices. The effects of two internal standards (CsCl and LiCl) on the reproducibility of Na quantification were explored using in-house fabricated microchips. Five concentrations of NaCl were analyzed consecutively over five days, resulting in a dataset of 900 electropherograms with which to draw reliable conclusions from. We find internal standards can strongly improve the precision of disposable devices.

Original language	Undefined
Title of host publication	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Place of Publication	San Diego
Publisher	Chemical and Biochemical Society
Pages	2405-2407
Number of pages	3
ISBN (Print)	978-0-9798064-7-6
Publication status	Published - 26 Oct 2014
Event	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014 - Henry B. Gonzalez Convention Center, San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014 Conference number: 18

Publication series

Name	MicroTAS 2014
Publisher	Chemical and Biochemical Society
ISSN (Print)	1556-5904

Conference

Conference	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014
Abbreviated title	MicroTAS 2014
Country/Territory	United States
City	San Antonio
Period	26/10/14 → 30/10/14

Keywords

EWI-25609
METIS-309841
IR-93841

Access to Document

http://eprints.eemcs.utwente.nl/secure2/25609/01/798_0076.pdf

Cite this

Bidulock, A. C. E., van den Berg, A., & Eijkel, J. C. T. (2014). Improved precision using internal standards in disposable point-of-care capillary electrophoresis devices. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 2405-2407). (MicroTAS 2014). Chemical and Biochemical Society. http://eprints.eemcs.utwente.nl/secure2/25609/01/798_0076.pdf

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title = "Improved precision using internal standards in disposable point-of-care capillary electrophoresis devices",

abstract = "This paper presents a systematic investigation of the viability of using internal standards to correct for chip-to-chip imprecision in disposable microchip capillary electrophoresis (μCE) devices. The effects of two internal standards (CsCl and LiCl) on the reproducibility of Na quantification were explored using in-house fabricated microchips. Five concentrations of NaCl were analyzed consecutively over five days, resulting in a dataset of 900 electropherograms with which to draw reliable conclusions from. We find internal standards can strongly improve the precision of disposable devices.",

keywords = "EWI-25609, METIS-309841, IR-93841",

author = "A.C.E. Bidulock and {van den Berg}, Albert and Eijkel, {Jan C.T.}",

note = "eemcs-eprint-25609 ; 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",

year = "2014",

month = oct,

day = "26",

language = "Undefined",

isbn = "978-0-9798064-7-6",

series = "MicroTAS 2014",

publisher = "Chemical and Biochemical Society",

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booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

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Bidulock, ACE, van den Berg, A & Eijkel, JCT 2014, Improved precision using internal standards in disposable point-of-care capillary electrophoresis devices. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. MicroTAS 2014, Chemical and Biochemical Society, San Diego, pp. 2405-2407, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2014, San Antonio, Texas, United States, 26/10/14. <http://eprints.eemcs.utwente.nl/secure2/25609/01/798_0076.pdf>

Improved precision using internal standards in disposable point-of-care capillary electrophoresis devices. / Bidulock, A.C.E.; van den Berg, Albert ; Eijkel, Jan C.T.
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. San Diego: Chemical and Biochemical Society, 2014. p. 2405-2407 (MicroTAS 2014).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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N2 - This paper presents a systematic investigation of the viability of using internal standards to correct for chip-to-chip imprecision in disposable microchip capillary electrophoresis (μCE) devices. The effects of two internal standards (CsCl and LiCl) on the reproducibility of Na quantification were explored using in-house fabricated microchips. Five concentrations of NaCl were analyzed consecutively over five days, resulting in a dataset of 900 electropherograms with which to draw reliable conclusions from. We find internal standards can strongly improve the precision of disposable devices.

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KW - METIS-309841

KW - IR-93841

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