Improved quantification for point-of-care capillary electrophoresis by adding an internal standard to the background electrolyte

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    Abstract

    This paper investigates the viability of incorporating an internal standard in the background electrolyte (BGE), versus including it in the sample, to correct for chip-to-chip imprecision in disposable microchip capillary electrophoresis (μCE) devices. Two internal standards investigated previously[1] as sample-added standards (CsCl and LiCl) were now added to the BGE, and their effects on the reproducibility of Na quantification were explored. The in-house fabricated microchips, electrophoretic protocols, and solution compositions were the same in both experiment sets, allowing us to reliably compare the two methods.
    Original languageUndefined
    Title of host publicationThe 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2015
    PublisherChemical and Biological Micro Systems Society
    Pages1984-1986
    Number of pages3
    ISBN (Print)978-0-9798064-8-3
    Publication statusPublished - 25 Oct 2015
    Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015 - Hwabaek International Convention Center (HICO), Gyeongju, Korea, Republic of
    Duration: 25 Oct 201529 Oct 2015
    Conference number: 19
    http://www.rsc.org/images/LOC/2015/PDFs/Copyrite.pdf

    Publication series

    NameProceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS
    PublisherChemical and Biological Microsystems Society
    ISSN (Print)1556-5904

    Conference

    Conference19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015
    Abbreviated titleMicroTas 2015
    CountryKorea, Republic of
    CityGyeongju
    Period25/10/1529/10/15
    Internet address

    Keywords

    • EWI-26558
    • Microchip Capillary Electrophoresis
    • IR-98707
    • Precision
    • METIS-315092
    • Reproducibility

    Cite this

    Bidulock, A. C. E., van den Berg, A., & Eijkel, J. C. T. (2015). Improved quantification for point-of-care capillary electrophoresis by adding an internal standard to the background electrolyte. In The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2015 (pp. 1984-1986). (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS). Chemical and Biological Micro Systems Society.