Improved quantification for point-of-care capillary electrophoresis by adding an internal standard to the background electrolyte

A.C.E. Bidulock; Albert van den Berg; Jan C.T. Eijkel

Improved quantification for point-of-care capillary electrophoresis by adding an internal standard to the background electrolyte

A.C.E. Bidulock, Albert van den Berg, Jan C.T. Eijkel

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

This paper investigates the viability of incorporating an internal standard in the background electrolyte (BGE), versus including it in the sample, to correct for chip-to-chip imprecision in disposable microchip capillary electrophoresis (μCE) devices. Two internal standards investigated previously[1] as sample-added standards (CsCl and LiCl) were now added to the BGE, and their effects on the reproducibility of Na quantification were explored. The in-house fabricated microchips, electrophoretic protocols, and solution compositions were the same in both experiment sets, allowing us to reliably compare the two methods.

Original language	Undefined
Title of host publication	The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2015
Publisher	The Chemical and Biological Microsystems Society
Pages	1984-1986
Number of pages	3
ISBN (Print)	978-0-9798064-8-3
Publication status	Published - 25 Oct 2015
Event	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015 - Hwabaek International Convention Center (HICO), Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015 Conference number: 19 http://www.rsc.org/images/LOC/2015/PDFs/Copyrite.pdf

Publication series

Name	Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS
Publisher	Chemical and Biological Microsystems Society
ISSN (Print)	1556-5904

Conference

Conference	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015
Abbreviated title	MicroTas 2015
Country/Territory	Korea, Republic of
City	Gyeongju
Period	25/10/15 → 29/10/15
Internet address	http://www.rsc.org/images/LOC/2015/PDFs/Copyrite.pdf

Keywords

EWI-26558
Microchip Capillary Electrophoresis
IR-98707
Precision
METIS-315092
Reproducibility

Access to Document

Cite this

Bidulock, A. C. E., van den Berg, A., & Eijkel, J. C. T. (2015). Improved quantification for point-of-care capillary electrophoresis by adding an internal standard to the background electrolyte. In The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2015 (pp. 1984-1986). (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS). The Chemical and Biological Microsystems Society. http://eprints.eemcs.utwente.nl/secure2/26558/01/1984_M.574h.pdf

Bidulock, A.C.E. ; van den Berg, Albert ; Eijkel, Jan C.T. / Improved quantification for point-of-care capillary electrophoresis by adding an internal standard to the background electrolyte. The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2015. The Chemical and Biological Microsystems Society, 2015. pp. 1984-1986 (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS).

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title = "Improved quantification for point-of-care capillary electrophoresis by adding an internal standard to the background electrolyte",

abstract = "This paper investigates the viability of incorporating an internal standard in the background electrolyte (BGE), versus including it in the sample, to correct for chip-to-chip imprecision in disposable microchip capillary electrophoresis (μCE) devices. Two internal standards investigated previously[1] as sample-added standards (CsCl and LiCl) were now added to the BGE, and their effects on the reproducibility of Na quantification were explored. The in-house fabricated microchips, electrophoretic protocols, and solution compositions were the same in both experiment sets, allowing us to reliably compare the two methods.",

keywords = "EWI-26558, Microchip Capillary Electrophoresis, IR-98707, Precision, METIS-315092, Reproducibility",

author = "A.C.E. Bidulock and {van den Berg}, Albert and Eijkel, {Jan C.T.}",

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series = "Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS",

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pages = "1984--1986",

booktitle = "The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2015",

note = "19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015 ; Conference date: 25-10-2015 Through 29-10-2015",

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Bidulock, ACE, van den Berg, A & Eijkel, JCT 2015, Improved quantification for point-of-care capillary electrophoresis by adding an internal standard to the background electrolyte. in The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2015. Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS, The Chemical and Biological Microsystems Society, pp. 1984-1986, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015, Gyeongju, Korea, Republic of, 25/10/15. <http://eprints.eemcs.utwente.nl/secure2/26558/01/1984_M.574h.pdf>

Improved quantification for point-of-care capillary electrophoresis by adding an internal standard to the background electrolyte. / Bidulock, A.C.E.; van den Berg, Albert ; Eijkel, Jan C.T.
The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2015. The Chemical and Biological Microsystems Society, 2015. p. 1984-1986 (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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T1 - Improved quantification for point-of-care capillary electrophoresis by adding an internal standard to the background electrolyte

AU - Bidulock, A.C.E.

AU - van den Berg, Albert

AU - Eijkel, Jan C.T.

N1 - Conference code: 19

PY - 2015/10/25

Y1 - 2015/10/25

N2 - This paper investigates the viability of incorporating an internal standard in the background electrolyte (BGE), versus including it in the sample, to correct for chip-to-chip imprecision in disposable microchip capillary electrophoresis (μCE) devices. Two internal standards investigated previously[1] as sample-added standards (CsCl and LiCl) were now added to the BGE, and their effects on the reproducibility of Na quantification were explored. The in-house fabricated microchips, electrophoretic protocols, and solution compositions were the same in both experiment sets, allowing us to reliably compare the two methods.

AB - This paper investigates the viability of incorporating an internal standard in the background electrolyte (BGE), versus including it in the sample, to correct for chip-to-chip imprecision in disposable microchip capillary electrophoresis (μCE) devices. Two internal standards investigated previously[1] as sample-added standards (CsCl and LiCl) were now added to the BGE, and their effects on the reproducibility of Na quantification were explored. The in-house fabricated microchips, electrophoretic protocols, and solution compositions were the same in both experiment sets, allowing us to reliably compare the two methods.

KW - EWI-26558

KW - Microchip Capillary Electrophoresis

KW - IR-98707

KW - Precision

KW - METIS-315092

KW - Reproducibility

M3 - Conference contribution

SN - 978-0-9798064-8-3

T3 - Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS

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BT - The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2015

PB - The Chemical and Biological Microsystems Society

T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015

Y2 - 25 October 2015 through 29 October 2015

ER -

Bidulock ACE, van den Berg A , Eijkel JCT. Improved quantification for point-of-care capillary electrophoresis by adding an internal standard to the background electrolyte. In The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2015. The Chemical and Biological Microsystems Society. 2015. p. 1984-1986. (Proceedings International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS).