Improving cellulase production by Aspergillus niger using adaptive evolution

Aleksandrina Patyshakuliyeva; Mark Arentshorst; Iris E. Allijn; Arthur F.J. Ram; Ronald P. de Vries; Isabelle Benoit Gelber

doi:10.1007/s10529-016-2060-0

Improving cellulase production by Aspergillus niger using adaptive evolution

Aleksandrina Patyshakuliyeva, Mark Arentshorst, Iris E. Allijn, Arthur F.J. Ram, Ronald P. de Vries, Isabelle Benoit Gelber^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Objectives: To evaluate the potential of adaptive evolution as a tool in generating strains with an improved production of plant biomass degrading enzymes. Results: An Aspergillus niger cellulase mutant was obtained by adaptive evolution. Physiological properties of this mutant revealed a five times higher cellulose production than the parental strain. Transcriptomic analysis revealed that the expression of noxR, encoding the regulatory subunit of the NADPH oxidase complex, was reduced in the mutant compared to the parental strain. Subsequent analysis of a noxR knockout strain showed the same phenotypic effect as observed for the evolution mutant, confirming the role of NoxR in cellulose degradation. Conclusions: Adaptive evolution is an efficient approach to modify a strain and activate genes involved in polysaccharide degradation.

Original language	English
Pages (from-to)	969-974
Number of pages	6
Journal	Biotechnology letters
Volume	38
Issue number	6
Early online date	15 Feb 2016
DOIs	https://doi.org/10.1007/s10529-016-2060-0
Publication status	Published - Jun 2016
Externally published	Yes

Keywords

Adaptive evolution
Aspergillus niger
Cellulose
NADPH oxidase
NoxR

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Patyshakuliyeva2016_Article_ImprovingCellulaseProductionByFinal published version, 788 KBLicence: CC BY

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title = "Improving cellulase production by Aspergillus niger using adaptive evolution",

abstract = "Objectives: To evaluate the potential of adaptive evolution as a tool in generating strains with an improved production of plant biomass degrading enzymes. Results: An Aspergillus niger cellulase mutant was obtained by adaptive evolution. Physiological properties of this mutant revealed a five times higher cellulose production than the parental strain. Transcriptomic analysis revealed that the expression of noxR, encoding the regulatory subunit of the NADPH oxidase complex, was reduced in the mutant compared to the parental strain. Subsequent analysis of a noxR knockout strain showed the same phenotypic effect as observed for the evolution mutant, confirming the role of NoxR in cellulose degradation. Conclusions: Adaptive evolution is an efficient approach to modify a strain and activate genes involved in polysaccharide degradation.",

keywords = "Adaptive evolution, Aspergillus niger, Cellulose, NADPH oxidase, NoxR",

author = "Aleksandrina Patyshakuliyeva and Mark Arentshorst and Allijn, {Iris E.} and Ram, {Arthur F.J.} and {de Vries}, {Ronald P.} and Gelber, {Isabelle Benoit}",

year = "2016",

month = jun,

doi = "10.1007/s10529-016-2060-0",

language = "English",

volume = "38",

pages = "969--974",

journal = "Biotechnology letters",

issn = "0141-5492",

publisher = "Springer",

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TY - JOUR

T1 - Improving cellulase production by Aspergillus niger using adaptive evolution

AU - Patyshakuliyeva, Aleksandrina

AU - Arentshorst, Mark

AU - Allijn, Iris E.

AU - Ram, Arthur F.J.

AU - de Vries, Ronald P.

AU - Gelber, Isabelle Benoit

PY - 2016/6

Y1 - 2016/6

N2 - Objectives: To evaluate the potential of adaptive evolution as a tool in generating strains with an improved production of plant biomass degrading enzymes. Results: An Aspergillus niger cellulase mutant was obtained by adaptive evolution. Physiological properties of this mutant revealed a five times higher cellulose production than the parental strain. Transcriptomic analysis revealed that the expression of noxR, encoding the regulatory subunit of the NADPH oxidase complex, was reduced in the mutant compared to the parental strain. Subsequent analysis of a noxR knockout strain showed the same phenotypic effect as observed for the evolution mutant, confirming the role of NoxR in cellulose degradation. Conclusions: Adaptive evolution is an efficient approach to modify a strain and activate genes involved in polysaccharide degradation.

AB - Objectives: To evaluate the potential of adaptive evolution as a tool in generating strains with an improved production of plant biomass degrading enzymes. Results: An Aspergillus niger cellulase mutant was obtained by adaptive evolution. Physiological properties of this mutant revealed a five times higher cellulose production than the parental strain. Transcriptomic analysis revealed that the expression of noxR, encoding the regulatory subunit of the NADPH oxidase complex, was reduced in the mutant compared to the parental strain. Subsequent analysis of a noxR knockout strain showed the same phenotypic effect as observed for the evolution mutant, confirming the role of NoxR in cellulose degradation. Conclusions: Adaptive evolution is an efficient approach to modify a strain and activate genes involved in polysaccharide degradation.

KW - Adaptive evolution

KW - Aspergillus niger

KW - Cellulose

KW - NADPH oxidase

KW - NoxR

U2 - 10.1007/s10529-016-2060-0

DO - 10.1007/s10529-016-2060-0

M3 - Article

C2 - 26879082

AN - SCOPUS:84958254611

SN - 0141-5492

VL - 38

SP - 969

EP - 974

JO - Biotechnology letters

JF - Biotechnology letters

IS - 6

ER -

Improving cellulase production by Aspergillus niger using adaptive evolution

Abstract

Keywords

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