Abstract
We report a non-invasive approach using scanning electrochemical microscopy for monitoring microtissue differentiation through the indirect detection of alkaline phosphatase. C2C12 microtissues produced in microwell arrays -stamped in commercially available polystyrene Petri dishes- are treated with 300 ng/mL bone morphogenic protein 2 to induce their differentiation into osteoblasts. Alkaline phosphatase in differentiated cells converts an electrochemically inactive substrate into an electrochemically active product, which is subsequently oxidized in the tissue vicinity using the SECM tip. Thereby, tissue differentiation can be monitored in a non-invasive and continuous way in live microtissues, while eliminating tedious and time-consuming preparation and cell fixation.
| Original language | English |
|---|---|
| Title of host publication | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
| Editors | R. Zengerle |
| Place of Publication | Freiburg |
| Publisher | The Printing House |
| Pages | 254-256 |
| Number of pages | 3 |
| ISBN (Print) | 978-0-9798064-6-9 |
| Publication status | Published - 27 Oct 2013 |
| Event | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2013 - Freiburg, Germany Duration: 27 Oct 2013 → 31 Oct 2013 Conference number: 17 |
Publication series
| Name | MicroTAS |
|---|---|
| Publisher | The Printing House |
| Volume | 2013 |
| ISSN (Print) | 1556-5904 |
Conference
| Conference | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2013 |
|---|---|
| Abbreviated title | MicroTAS |
| Country | Germany |
| City | Freiburg |
| Period | 27/10/13 → 31/10/13 |
Keywords
- Alkaline phosphatase
- Differentiation assay
- Secm
- Stamped petri dishes