In situ non-invasive electrochemical monitoring of microtissue differentiation in microwell arrays

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    Abstract

    We report a non-invasive approach using scanning electrochemical microscopy for monitoring microtissue differentiation through the indirect detection of alkaline phosphatase. C2C12 microtissues produced in microwell arrays -stamped in commercially available polystyrene Petri dishes- are treated with 300 ng/mL bone morphogenic protein 2 to induce their differentiation into osteoblasts. Alkaline phosphatase in differentiated cells converts an electrochemically inactive substrate into an electrochemically active product, which is subsequently oxidized in the tissue vicinity using the SECM tip. Thereby, tissue differentiation can be monitored in a non-invasive and continuous way in live microtissues, while eliminating tedious and time-consuming preparation and cell fixation.
    Original languageEnglish
    Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
    EditorsR. Zengerle
    Place of PublicationFreiburg
    PublisherThe Printing House
    Pages254-256
    Number of pages3
    ISBN (Print)978-0-9798064-6-9
    Publication statusPublished - 27 Oct 2013
    Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2013 - Freiburg, Germany
    Duration: 27 Oct 201331 Oct 2013
    Conference number: 17

    Publication series

    NameMicroTAS
    PublisherThe Printing House
    Volume2013
    ISSN (Print)1556-5904

    Conference

    Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2013
    Abbreviated titleMicroTAS
    CountryGermany
    CityFreiburg
    Period27/10/1331/10/13

    Keywords

    • Alkaline phosphatase
    • Differentiation assay
    • Secm
    • Stamped petri dishes

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