In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels

M Bongio; M.R. Nejadnik; Zeinab Tahmasebi Birgani; Pamela Habibovic; L.A. Kinard; F.K. Kasper; A.G. Mikos; J.A. Jansen; S.C.G. Leeuwenburgh; J.J.J.P. van den Beucken

doi:10.1002/mabi.201200474

In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels

M Bongio, M.R. Nejadnik, Zeinab Tahmasebi Birgani, Pamela Habibovic, L.A. Kinard, F.K. Kasper, A.G. Mikos, J.A. Jansen, S.C.G. Leeuwenburgh, J.J.J.P. van den Beucken

Faculty of Science and Technology

Research output: Contribution to journal › Article › Academic › peer-review

9 Citations (Scopus)

Abstract

The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10 mg · ml−1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10 mM calcium glycerophosphate (Ca–GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (≈5–20 µm) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.

Original language	English
Pages (from-to)	777-788
Journal	Macromolecular bioscience
Volume	13
Issue number	6
DOIs	https://doi.org/10.1002/mabi.201200474
Publication status	Published - 10 Apr 2013

Keywords

METIS-295629
IR-85412

Access to Document

10.1002/mabi.201200474

Fingerprint Dive into the research topics of 'In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels'. Together they form a unique fingerprint.

Cite this

Bongio, M., Nejadnik, M. R., Tahmasebi Birgani, Z., Habibovic, P., Kinard, L. A., Kasper, F. K., ... van den Beucken, J. J. J. P. (2013). In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels. Macromolecular bioscience, 13(6), 777-788. https://doi.org/10.1002/mabi.201200474

@article{dbbe27921fc745ada37e9d9fa43ed64f,

title = "In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels",

abstract = "The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10 mg · ml−1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10 mM calcium glycerophosphate (Ca–GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (≈5–20 µm) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.",

keywords = "METIS-295629, IR-85412",

author = "M Bongio and M.R. Nejadnik and {Tahmasebi Birgani}, Zeinab and Pamela Habibovic and L.A. Kinard and F.K. Kasper and A.G. Mikos and J.A. Jansen and S.C.G. Leeuwenburgh and {van den Beucken}, J.J.J.P.",

note = "Article in press",

year = "2013",

month = "4",

day = "10",

doi = "10.1002/mabi.201200474",

language = "English",

volume = "13",

pages = "777--788",

journal = "Macromolecular bioscience",

issn = "1616-5187",

publisher = "Wiley-VCH Verlag",

number = "6",

}

In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels. / Bongio, M; Nejadnik, M.R.; Tahmasebi Birgani, Zeinab; Habibovic, Pamela; Kinard, L.A.; Kasper, F.K.; Mikos, A.G.; Jansen, J.A.; Leeuwenburgh, S.C.G.; van den Beucken, J.J.J.P.

In: Macromolecular bioscience, Vol. 13, No. 6, 10.04.2013, p. 777-788.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels

AU - Bongio, M

AU - Nejadnik, M.R.

AU - Tahmasebi Birgani, Zeinab

AU - Habibovic, Pamela

AU - Kinard, L.A.

AU - Kasper, F.K.

AU - Mikos, A.G.

AU - Jansen, J.A.

AU - Leeuwenburgh, S.C.G.

AU - van den Beucken, J.J.J.P.

N1 - Article in press

PY - 2013/4/10

Y1 - 2013/4/10

N2 - The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10 mg · ml−1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10 mM calcium glycerophosphate (Ca–GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (≈5–20 µm) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.

AB - The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10 mg · ml−1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10 mM calcium glycerophosphate (Ca–GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (≈5–20 µm) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.

KW - METIS-295629

KW - IR-85412

U2 - 10.1002/mabi.201200474

DO - 10.1002/mabi.201200474

M3 - Article

VL - 13

SP - 777

EP - 788

JO - Macromolecular bioscience

JF - Macromolecular bioscience

SN - 1616-5187

IS - 6

ER -