In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels

M Bongio; M.R. Nejadnik; Zeinab Tahmasebi Birgani; Pamela Habibovic; L.A. Kinard; F.K. Kasper; A.G. Mikos; J.A. Jansen; S.C.G. Leeuwenburgh; J.J.J.P. van den Beucken

doi:10.1002/mabi.201200474

In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels

M Bongio, M.R. Nejadnik, Zeinab Tahmasebi Birgani, Pamela Habibovic, L.A. Kinard, F.K. Kasper, A.G. Mikos, J.A. Jansen, S.C.G. Leeuwenburgh, J.J.J.P. van den Beucken

Faculty of Science and Technology

Research output: Contribution to journal › Article › Academic › peer-review

9 Citations (Scopus)

Abstract

The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10 mg · ml−1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10 mM calcium glycerophosphate (Ca–GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (≈5–20 µm) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.

Original language	English
Pages (from-to)	777-788
Journal	Macromolecular bioscience
Volume	13
Issue number	6
DOIs	https://doi.org/10.1002/mabi.201200474
Publication status	Published - 10 Apr 2013

Keywords

METIS-295629
IR-85412

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10.1002/mabi.201200474

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Bongio, M., Nejadnik, M. R., Tahmasebi Birgani, Z., Habibovic, P., Kinard, L. A., Kasper, F. K., Mikos, A. G., Jansen, J. A., Leeuwenburgh, S. C. G., & van den Beucken, J. J. J. P. (2013). In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels. Macromolecular bioscience, 13(6), 777-788. https://doi.org/10.1002/mabi.201200474

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title = "In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels",

abstract = "The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10 mg · ml−1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10 mM calcium glycerophosphate (Ca–GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (≈5–20 µm) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.",

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author = "M Bongio and M.R. Nejadnik and {Tahmasebi Birgani}, Zeinab and Pamela Habibovic and L.A. Kinard and F.K. Kasper and A.G. Mikos and J.A. Jansen and S.C.G. Leeuwenburgh and {van den Beucken}, J.J.J.P.",

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In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels. / Bongio, M; Nejadnik, M.R.; Tahmasebi Birgani, Zeinab; Habibovic, Pamela; Kinard, L.A.; Kasper, F.K.; Mikos, A.G.; Jansen, J.A.; Leeuwenburgh, S.C.G.; van den Beucken, J.J.J.P.

In: Macromolecular bioscience, Vol. 13, No. 6, 10.04.2013, p. 777-788.

Research output: Contribution to journal › Article › Academic › peer-review

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T1 - In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels

AU - Bongio, M

AU - Nejadnik, M.R.

AU - Tahmasebi Birgani, Zeinab

AU - Habibovic, Pamela

AU - Kinard, L.A.

AU - Kasper, F.K.

AU - Mikos, A.G.

AU - Jansen, J.A.

AU - Leeuwenburgh, S.C.G.

AU - van den Beucken, J.J.J.P.

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AB - The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10 mg · ml−1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10 mM calcium glycerophosphate (Ca–GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (≈5–20 µm) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.

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