In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels

M Bongio, M.R. Nejadnik, Zeinab Tahmasebi Birgani, Pamela Habibovic, L.A. Kinard, F.K. Kasper, A.G. Mikos, J.A. Jansen, S.C.G. Leeuwenburgh, J.J.J.P. van den Beucken

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9 Citations (Scopus)

Abstract

The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10 mg · ml−1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10 mM calcium glycerophosphate (Ca–GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (≈5–20 µm) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.
Original languageEnglish
Pages (from-to)777-788
JournalMacromolecular bioscience
Volume13
Issue number6
DOIs
Publication statusPublished - 10 Apr 2013

Keywords

  • METIS-295629
  • IR-85412

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