TY - JOUR
T1 - In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels
AU - Bongio, M
AU - Nejadnik, M.R.
AU - Tahmasebi Birgani, Zeinab
AU - Habibovic, Pamela
AU - Kinard, L.A.
AU - Kasper, F.K.
AU - Mikos, A.G.
AU - Jansen, J.A.
AU - Leeuwenburgh, S.C.G.
AU - van den Beucken, J.J.J.P.
N1 - Article in press
PY - 2013/4/10
Y1 - 2013/4/10
N2 - The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10 mg · ml−1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10 mM calcium glycerophosphate (Ca–GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (≈5–20 µm) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.
AB - The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10 mg · ml−1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10 mM calcium glycerophosphate (Ca–GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (≈5–20 µm) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.
KW - METIS-295629
KW - IR-85412
U2 - 10.1002/mabi.201200474
DO - 10.1002/mabi.201200474
M3 - Article
VL - 13
SP - 777
EP - 788
JO - Macromolecular bioscience
JF - Macromolecular bioscience
SN - 1616-5187
IS - 6
ER -