In vitro imaging of bacteria using 18F-fluorodeoxyglucose micro positron emission tomography

Marjolein Heuker, Jürgen W.A. Sijbesma, Rocío Aguilar Suárez, Johan R. De Jong, Hendrikus H. Boersma, Gert Luurtsema, Philip H. Elsinga, Andor W.J.M. Glaudemans, Gooitzen M. van Dam, Jan Maarten van Dijl, Riemer H.J.A. Slart, Marleen van Oosten

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Abstract

Positron emission tomography (PET) with fluorine-18-fluorodeoxyglucose (18F-FDG) can be applied to detect infection and inflammation. However, it was so far not known to what extent bacterial pathogens may contribute to the PET signal. Therefore, we investigated whether clinical isolates of frequently encountered bacterial pathogens take up 18F-FDG in vitro, and whether FDG inhibits bacterial growth as previously shown for 2-deoxy-glucose. 22 isolates of Gram-positive and Gram-negative bacterial pathogens implicated in fever and inflammation were incubated with 18F-FDG and uptake of 18F-FDG was assessed by gamma-counting and μPET imaging. Possible growth inhibition by FDG was assayed with Staphylococcus aureus and the Gram-positive model bacterium Bacillus subtilis. The results show that all tested isolates accumulated 18F-FDG actively. Further, 18F-FDG uptake was hampered in B. subtilis pts mutants impaired in glucose uptake. FDG inhibited growth of S. aureus and B. subtilis only to minor extents, and this effect was abrogated by pts mutations in B. subtilis. These observations imply that bacteria may contribute to the signals observed in FDG-PET infection imaging in vivo. Active bacterial FDG uptake is corroborated by the fact that the B. subtilis phosphotransferase system is needed for 18F-FDG uptake, while pts mutations protect against growth inhibition by FDG.

Original languageEnglish
Article number4973
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - 1 Dec 2017

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Fluorodeoxyglucose F18
Positron-Emission Tomography
Bacteria
Bacillus subtilis
Growth
Staphylococcus aureus
Inflammation
Glucose
Mutation
In Vitro Techniques
Gram-Positive Bacteria
Infection
Fever
Phosphotransferases

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Heuker, M., Sijbesma, J. W. A., Suárez, R. A., De Jong, J. R., Boersma, H. H., Luurtsema, G., ... van Oosten, M. (2017). In vitro imaging of bacteria using 18F-fluorodeoxyglucose micro positron emission tomography. Scientific reports, 7(1), [4973]. https://doi.org/10.1038/s41598-017-05403-z
Heuker, Marjolein ; Sijbesma, Jürgen W.A. ; Suárez, Rocío Aguilar ; De Jong, Johan R. ; Boersma, Hendrikus H. ; Luurtsema, Gert ; Elsinga, Philip H. ; Glaudemans, Andor W.J.M. ; van Dam, Gooitzen M. ; van Dijl, Jan Maarten ; Slart, Riemer H.J.A. ; van Oosten, Marleen. / In vitro imaging of bacteria using 18F-fluorodeoxyglucose micro positron emission tomography. In: Scientific reports. 2017 ; Vol. 7, No. 1.
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abstract = "Positron emission tomography (PET) with fluorine-18-fluorodeoxyglucose (18F-FDG) can be applied to detect infection and inflammation. However, it was so far not known to what extent bacterial pathogens may contribute to the PET signal. Therefore, we investigated whether clinical isolates of frequently encountered bacterial pathogens take up 18F-FDG in vitro, and whether FDG inhibits bacterial growth as previously shown for 2-deoxy-glucose. 22 isolates of Gram-positive and Gram-negative bacterial pathogens implicated in fever and inflammation were incubated with 18F-FDG and uptake of 18F-FDG was assessed by gamma-counting and μPET imaging. Possible growth inhibition by FDG was assayed with Staphylococcus aureus and the Gram-positive model bacterium Bacillus subtilis. The results show that all tested isolates accumulated 18F-FDG actively. Further, 18F-FDG uptake was hampered in B. subtilis pts mutants impaired in glucose uptake. FDG inhibited growth of S. aureus and B. subtilis only to minor extents, and this effect was abrogated by pts mutations in B. subtilis. These observations imply that bacteria may contribute to the signals observed in FDG-PET infection imaging in vivo. Active bacterial FDG uptake is corroborated by the fact that the B. subtilis phosphotransferase system is needed for 18F-FDG uptake, while pts mutations protect against growth inhibition by FDG.",
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Heuker, M, Sijbesma, JWA, Suárez, RA, De Jong, JR, Boersma, HH, Luurtsema, G, Elsinga, PH, Glaudemans, AWJM, van Dam, GM, van Dijl, JM, Slart, RHJA & van Oosten, M 2017, 'In vitro imaging of bacteria using 18F-fluorodeoxyglucose micro positron emission tomography' Scientific reports, vol. 7, no. 1, 4973. https://doi.org/10.1038/s41598-017-05403-z

In vitro imaging of bacteria using 18F-fluorodeoxyglucose micro positron emission tomography. / Heuker, Marjolein; Sijbesma, Jürgen W.A.; Suárez, Rocío Aguilar; De Jong, Johan R.; Boersma, Hendrikus H.; Luurtsema, Gert; Elsinga, Philip H.; Glaudemans, Andor W.J.M.; van Dam, Gooitzen M.; van Dijl, Jan Maarten; Slart, Riemer H.J.A.; van Oosten, Marleen.

In: Scientific reports, Vol. 7, No. 1, 4973, 01.12.2017.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - In vitro imaging of bacteria using 18F-fluorodeoxyglucose micro positron emission tomography

AU - Heuker, Marjolein

AU - Sijbesma, Jürgen W.A.

AU - Suárez, Rocío Aguilar

AU - De Jong, Johan R.

AU - Boersma, Hendrikus H.

AU - Luurtsema, Gert

AU - Elsinga, Philip H.

AU - Glaudemans, Andor W.J.M.

AU - van Dam, Gooitzen M.

AU - van Dijl, Jan Maarten

AU - Slart, Riemer H.J.A.

AU - van Oosten, Marleen

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Positron emission tomography (PET) with fluorine-18-fluorodeoxyglucose (18F-FDG) can be applied to detect infection and inflammation. However, it was so far not known to what extent bacterial pathogens may contribute to the PET signal. Therefore, we investigated whether clinical isolates of frequently encountered bacterial pathogens take up 18F-FDG in vitro, and whether FDG inhibits bacterial growth as previously shown for 2-deoxy-glucose. 22 isolates of Gram-positive and Gram-negative bacterial pathogens implicated in fever and inflammation were incubated with 18F-FDG and uptake of 18F-FDG was assessed by gamma-counting and μPET imaging. Possible growth inhibition by FDG was assayed with Staphylococcus aureus and the Gram-positive model bacterium Bacillus subtilis. The results show that all tested isolates accumulated 18F-FDG actively. Further, 18F-FDG uptake was hampered in B. subtilis pts mutants impaired in glucose uptake. FDG inhibited growth of S. aureus and B. subtilis only to minor extents, and this effect was abrogated by pts mutations in B. subtilis. These observations imply that bacteria may contribute to the signals observed in FDG-PET infection imaging in vivo. Active bacterial FDG uptake is corroborated by the fact that the B. subtilis phosphotransferase system is needed for 18F-FDG uptake, while pts mutations protect against growth inhibition by FDG.

AB - Positron emission tomography (PET) with fluorine-18-fluorodeoxyglucose (18F-FDG) can be applied to detect infection and inflammation. However, it was so far not known to what extent bacterial pathogens may contribute to the PET signal. Therefore, we investigated whether clinical isolates of frequently encountered bacterial pathogens take up 18F-FDG in vitro, and whether FDG inhibits bacterial growth as previously shown for 2-deoxy-glucose. 22 isolates of Gram-positive and Gram-negative bacterial pathogens implicated in fever and inflammation were incubated with 18F-FDG and uptake of 18F-FDG was assessed by gamma-counting and μPET imaging. Possible growth inhibition by FDG was assayed with Staphylococcus aureus and the Gram-positive model bacterium Bacillus subtilis. The results show that all tested isolates accumulated 18F-FDG actively. Further, 18F-FDG uptake was hampered in B. subtilis pts mutants impaired in glucose uptake. FDG inhibited growth of S. aureus and B. subtilis only to minor extents, and this effect was abrogated by pts mutations in B. subtilis. These observations imply that bacteria may contribute to the signals observed in FDG-PET infection imaging in vivo. Active bacterial FDG uptake is corroborated by the fact that the B. subtilis phosphotransferase system is needed for 18F-FDG uptake, while pts mutations protect against growth inhibition by FDG.

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Heuker M, Sijbesma JWA, Suárez RA, De Jong JR, Boersma HH, Luurtsema G et al. In vitro imaging of bacteria using 18F-fluorodeoxyglucose micro positron emission tomography. Scientific reports. 2017 Dec 1;7(1). 4973. https://doi.org/10.1038/s41598-017-05403-z