In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications

Guillaume Pierre Rene Lajoinie, Ine De Cock, Constantin C. Coussios, Ine Lentacker, Severine le Gac, Eleanor Stride, Michel Versluis

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)
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Abstract

Besides their use as contrast agents for ultrasound imaging,microbubbles are increasingly studied for a wide range of therapeutic applications. In particular, their ability to enhance the uptake of drugs through the permeabilization of tissues and cell membranes shows great promise. In order to fully understand the numerous paths by which bubbles can interact with cells and the even larger number of possible biological responses from the cells, thorough and extensive work is necessary. In this review, we consider the range of experimental techniques implemented in in vitro studies with the aim of elucidating these microbubble-cell interactions. First of all, the variety of cell types and cell models available are discussed, emphasizing the need for more and more complex models replicating in vivo conditions together with experimental challenges associated with this increased complexity. Second, the different types of stabilized microbubbles and more recently developed droplets and particles are presented, followed by their acoustic or optical excitation methods. Finally, the techniques exploited to study the microbubble-cell interactions are reviewed. These techniques operate over a wide range of timescales, or even off-line, revealing particular aspects or subsequent effects of these interactions. Therefore, knowledge obtained from several techniques must be combined to elucidate the underlying processes.
Original languageEnglish
Article number011501
Pages (from-to)-
Number of pages25
JournalBiomicrofluidics
Volume10
Issue number1
DOIs
Publication statusPublished - 2016

Fingerprint

bubbles
Photoexcitation
Cell membranes
cells
Contrast Media
Ultrasonics
Acoustics
interactions
Tissue
Imaging techniques
Pharmaceutical Preparations
acoustic excitation
drugs
membranes
excitation

Keywords

  • METIS-315346
  • IR-100723

Cite this

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title = "In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications",
abstract = "Besides their use as contrast agents for ultrasound imaging,microbubbles are increasingly studied for a wide range of therapeutic applications. In particular, their ability to enhance the uptake of drugs through the permeabilization of tissues and cell membranes shows great promise. In order to fully understand the numerous paths by which bubbles can interact with cells and the even larger number of possible biological responses from the cells, thorough and extensive work is necessary. In this review, we consider the range of experimental techniques implemented in in vitro studies with the aim of elucidating these microbubble-cell interactions. First of all, the variety of cell types and cell models available are discussed, emphasizing the need for more and more complex models replicating in vivo conditions together with experimental challenges associated with this increased complexity. Second, the different types of stabilized microbubbles and more recently developed droplets and particles are presented, followed by their acoustic or optical excitation methods. Finally, the techniques exploited to study the microbubble-cell interactions are reviewed. These techniques operate over a wide range of timescales, or even off-line, revealing particular aspects or subsequent effects of these interactions. Therefore, knowledge obtained from several techniques must be combined to elucidate the underlying processes.",
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In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications. / Lajoinie, Guillaume Pierre Rene; De Cock, Ine; Coussios, Constantin C.; Lentacker, Ine; le Gac, Severine; Stride, Eleanor; Versluis, Michel.

In: Biomicrofluidics, Vol. 10, No. 1, 011501, 2016, p. -.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications

AU - Lajoinie, Guillaume Pierre Rene

AU - De Cock, Ine

AU - Coussios, Constantin C.

AU - Lentacker, Ine

AU - le Gac, Severine

AU - Stride, Eleanor

AU - Versluis, Michel

PY - 2016

Y1 - 2016

N2 - Besides their use as contrast agents for ultrasound imaging,microbubbles are increasingly studied for a wide range of therapeutic applications. In particular, their ability to enhance the uptake of drugs through the permeabilization of tissues and cell membranes shows great promise. In order to fully understand the numerous paths by which bubbles can interact with cells and the even larger number of possible biological responses from the cells, thorough and extensive work is necessary. In this review, we consider the range of experimental techniques implemented in in vitro studies with the aim of elucidating these microbubble-cell interactions. First of all, the variety of cell types and cell models available are discussed, emphasizing the need for more and more complex models replicating in vivo conditions together with experimental challenges associated with this increased complexity. Second, the different types of stabilized microbubbles and more recently developed droplets and particles are presented, followed by their acoustic or optical excitation methods. Finally, the techniques exploited to study the microbubble-cell interactions are reviewed. These techniques operate over a wide range of timescales, or even off-line, revealing particular aspects or subsequent effects of these interactions. Therefore, knowledge obtained from several techniques must be combined to elucidate the underlying processes.

AB - Besides their use as contrast agents for ultrasound imaging,microbubbles are increasingly studied for a wide range of therapeutic applications. In particular, their ability to enhance the uptake of drugs through the permeabilization of tissues and cell membranes shows great promise. In order to fully understand the numerous paths by which bubbles can interact with cells and the even larger number of possible biological responses from the cells, thorough and extensive work is necessary. In this review, we consider the range of experimental techniques implemented in in vitro studies with the aim of elucidating these microbubble-cell interactions. First of all, the variety of cell types and cell models available are discussed, emphasizing the need for more and more complex models replicating in vivo conditions together with experimental challenges associated with this increased complexity. Second, the different types of stabilized microbubbles and more recently developed droplets and particles are presented, followed by their acoustic or optical excitation methods. Finally, the techniques exploited to study the microbubble-cell interactions are reviewed. These techniques operate over a wide range of timescales, or even off-line, revealing particular aspects or subsequent effects of these interactions. Therefore, knowledge obtained from several techniques must be combined to elucidate the underlying processes.

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